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Citations for Stemcell Technologies :
251 - 300 of 4286 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated using the Direct Human PBMC Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol and washed/resuspended in custom RPMI-1640 deficient in biotin ...
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bioRxiv - Immunology 2020Quote: ... and enriched for CD8+ cells with an EasySep™ negatively selective magnetic isolation kit (STEMCELL Technologies). OT-I cells prepared for use in nuclei isolation experiments were then treated with CellTrace™ Violet reactive dye (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated at RT using SepMate PBMC Isolation Tubes (STEMCELL Technologies) by the UCSF Biospecimen Resource Program ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells were subsequently isolated using the Stemcell human CD8+ isolation kit (Stemcell Technology) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and T lymphocytes purified using Easy SepTM Mouse T cell isolation kit (STEMCELL Technologies, Cat# 19851A), as we described83 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lineage negative hematopoietic stem and progenitor cells (HSPCs) were purified using EasySep Isolation Kit (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lineage− cells were enriched by negative selection using the EasySep Streptavidin RapidSpheres Isolation Kit (StemCell Technologies) and MojoSort Magnets (BioLegend) ...
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bioRxiv - Immunology 2024Quote: Esophageal biopsies were dissociated (27) and CD45+ cells were removed using positive isolation beads (STEMCELL TECHNOLOGIES). The untouched epithelial-enriched cell fraction was washed with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... HSCs were enriched using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Viable T cells were enriched using the Easy Sep T cell isolation Kit followed by the Easy Sep Dead Cell removal Kit (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... total CD4+ T cells were isolated from PBMCs by negative magnetic separation using EasySep Human CD4+ T cell isolation kit (StemCell Technology). Following treatment for 5 days (Fig ...
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bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2019Quote: ... naïve CD44+ T cells were enriched by magnetic separation using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies). 55×104 T cells were cultured for 6 days in the presence of plate-bound αCD3ε (5 μg mL−1) ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve CD4+ T cells were then enriched from total CD4+ T cells through immuno-magnetic negative selection with the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies). Naïve CD4+ T cell post-isolation purity (CD4+CD45RA+CD45RO- ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T-cells (CD3+CD4+CD45RA+CD45RO-) were purified from PBMC using the EasySep Human CD4+ T-cell Isolation Kit (Stemcell Technologies) to >95% purity according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleens and lymph nodes using the EasySep mouse CD4+ T cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Human naïve T cells were isolated from PBMCs using the EasySep™ Human naïve CD4+ T Cell Isolation Kit Human (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of naïve (CD44loCD62Lhi) lymph node CD8+ T cells (isolated using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit, Stemcell Technologies) were washed with ice-cold PBS supplemented with 2% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary human CD3+ T cells were isolated from PBMCs by negative selection using EasySep Human T Cell Isolation Kit (StemCell Technologies) or by positive selection using Dynabeads Human T-Expander CD3/CD28 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... and subjected to a CD8+ T cell negative selection with the EasySepTM Mouse T cell isolation kit (STEMCELL Technologies cat#: 19853), according to the manufacturer’s protocol ...