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Citations for Stemcell Technologies :
51 - 86 of 86 citations for Celase GMP Collagenase Blend since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... mashed tissue suspension was further treated with ∼270U of prewarmed collagenase IV (STEMCELL Technologies, USA) in a cryotube vial for 90 min at 37°C followed by 0.05% trypsin-EDTA for 20 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Macroscopic lung nodules were dissected separately and digested in Collagenase Type IV (Stem Cell technologies; 07909) at 37°C for 1.5 h with rocking ...
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bioRxiv - Cancer Biology 2021Quote: ... media supplemented with 10% FBS (WISENT, ST-BRUNO, QC, Canada) and collagenase (STEMCELL Technologies, Vancouver, BC, Canada), and further dissociated in 5 mg/ml dispase for 2min ...
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bioRxiv - Bioengineering 2022Quote: ... printed constructs were incubated for 20 min in 5 mg mL-1 collagenase type I (STEMCELL Technologies) in PBS supplemented with 5% FBS and then lysed with RLT buffer ...
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bioRxiv - Genomics 2021Quote: ... Prostate lobes were minced with a sterile razor blade and resuspended in collagenase/hyaluronidase (#07912, Stemcell Technologies) diluted in DMEM/F-12 (#36254 ...
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bioRxiv - Cancer Biology 2020Quote: ... combination or vehicle control for 14 days were collected and digested using collagenase-hyaluronidase (Stem Cell Technologies) to obtain single-cell suspensions ...
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bioRxiv - Physiology 2023Quote: ... Homogenized livers were resuspended in 10mL HBSS containing 0.05% collagenase (Type IV; Stem cell technology; cat#07427) and the specimens were shaken for 20 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then enzymatically dissociated using 750 U/mL collagenase and 250 U/mL hyaluronidase (STEMCELL Technologies, 07912) and diluted in DMEM:F12 for 1.5 h ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumors (6–8 mm in diameter) were mechanically dissociated and digested with collagenase/hyaluronidase (#07912, STEMCELL Technologies) containing DNase I (4 U/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were minced into ∼1–2 mm pieces before digesting with 1 × Collagenase/Hyaluronidase (STEMCELL Technologies, Catalog #: 07912) and 100 μg/mL DNase I (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... single-cell suspensions from the tumors were prepared by enzymatic digestion with collagenase/hyaluronidase (Stem Cell Technologies, 07912) enzyme mix in serum-free Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2019Quote: ... Embryoid bodies (EBs) were generated from feeder-free iPSCs by incubating colonies with Collagenase Type IV (7909, StemCell Technologies) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Tumors were cut into small pieces with a razor blade and incubated with collagenase IV (STEMCELL Technologies, Kent, WA) on a rotator at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Media was changed daily and cells were split at a 1:6 ratio using Collagenase IV (Stemcell Technologies, #07909) every 5-6 days.
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bioRxiv - Cell Biology 2020Quote: Mammary glands from 10-16 weeks old C57BL/6J females were minced and dissociated using collagenase/hyaluronidase (STEMCELL Technologies) diluted (1:10 ...
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bioRxiv - Cancer Biology 2021Quote: ... Following enzymatic digestion of tumor tissues using 1x Collagenase/Hyaluronidase and DNase-1 (Cat # 07912 & 07900; Stem cell technologies), dissociated tumor cells were collected in a different tube and filtered through 40 µ strainer ...
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bioRxiv - Bioengineering 2023Quote: ... 1G4 PBMCs loaded onto pMHC nanovials were sorted based on calcein AM and NGFR signal and reconstituted with 0.75 mL media and 0.25 mL of 10 mg/mL Collagenase Type II solution (STEMCELL Technologies), followed by a 2 hour incubation at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumors were mechanically dissociated and digested for 1 h with 1x collagenase/hyaluronidase (10X Solution, Stem Cell Technology, Cambridge, MA) containing DNase I (2 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... YS were digested with collagenase I to dissociate the cells into single cell suspension and plated in methylcellulose medium (media M34334, StemCell Technologies) containing cytokines (SCF ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 tumors were extracted and minced with sterile razor blades and incubated for 2 h in the presence of Collagenase (1,000 units per sample) in sterile Epicult media (Stem Cell Technology). Cells were washed with sterile filtered PBS supplemented with 1% bovine serum albumin (PBS-BSA 1% ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Cancer Biology 2022Quote: ... glands were first digested overnight (∼16 hours) with gentle agitation at 37 °C in 250 μL Gentle Collagenase (Stemcell Technologies #07919) diluted in 2.25 mL of complete Basal Epicult media formulated according to manufacture instructions (Epicult Basal Medium Stemcell Technologies #05610 ...
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bioRxiv - Pathology 2023Quote: ... The dissociation solution was prepared by combining 30 mL of the freshly prepared stock collagenase solution with 20 mL of dispase in Hank’s balanced salt solution (Stemcell Technologies, #07913), resulting in a 60% collagenase ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were transferred with help of a sterile wide bore 200 µl pipette tip into a 1.8 ml cryo vial containing 1.2 ml A-mTE-D1 (300 IU ml-1 collagenase IV mixed with 100 IU ml-1 hyaluronidase; Stem Cell Technologies, 07912 ...
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bioRxiv - Cancer Biology 2019Quote: Bulk tumors were mechanically minced into a slurry with sterile scalpels and then enzymatically digested in Media 199 containing 300 U/ml Collagenase and 100 U/ml Hyaluronidase mixture (Stem Cell Technologies) and 125 U/ml DNAse-I (Cedarlane ...
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bioRxiv - Cancer Biology 2020Quote: ... Fourth and fifth mammary gland pairs were resected from mice and were enzymatically digested with collagenase/hyaluronidase (Stem Cell Technologies, cat #07919) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... Depending on the availability100 mm3-2000 mm3 cm tumor tissue piece was minced into smaller pieces and digested with 1x Collagenase & Hyaluronidase (# 7912, Stem Cell Technologies), or 1U/mL Dispase II (# D4693 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... before undergoing tissue dissociation with a GentleMACS™ dissociator (Miltenyi Biotech) using the manufacturer’s recommended protocol with media including Collagenase Type IV (STEMCELL Technologies, Cambridge MA) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
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bioRxiv - Developmental Biology 2022Quote: ... Roughly separated tubules were incubated in collagenase type IV in DMEM (5 mL for 1 testis pair, 1 mg/mL, STEMCELL Technologies Inc., Cat# 07909) at 37 °C while rotating ...