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Citations for Stemcell Technologies :
51 - 100 of 992 citations for CD28 Human Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Anti-CD3/CD28/CD2 Immunocult (Stemcell Technologies) was used for T-cell activation ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Neuroscience 2023Quote: ... plated cells were immediately supplemented with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies), or a combination of plate-bound anti-CD3 (OKT3 ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Cancer Biology 2021Quote: ... activated with CD3/CD28 T cell Activator reagent (STEMCELL Technologies, Vancouver, Canada) and transduced on day 1 with the CAR lentiviral particles in the presence of 1X Transdux (Systems Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: PDECs were treated with 25ul/mL anti-CD3/CD28/CD2 (Stemcell Technologies), 100ug/mL Atezolizumab (Selleck Chemicals) ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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bioRxiv - Immunology 2019Quote: ... containing 10% (vol/vol) FCS (StemCell Technologies), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... The T cell expansion medium was composed of 12.5ul/ml ImmunoCult CD3/CD28 (STEMCELL Technologies) and 100ng/ml IL-2 and 5ng/ml IL-15 (PeproTech EC Ltd) ...
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bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
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bioRxiv - Bioengineering 2019Quote: ... 5.0 × 106 cryopreserved T cells were expanded using CD3/CD28 T cell activator (StemCell Technologies) and standard conditions for 16 days to achieve sufficient cell numbers for the experimental workflow ...
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bioRxiv - Biochemistry 2023Quote: ... primary CD4+ T cells were activated with anti-CD3/CD28 antibodies (cat#10971, Stemcell Technologies). 1e6 activated primary CD4+ T cells were electroporated with 1μg PEmax variant mRNA ...
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bioRxiv - Immunology 2022Quote: ... Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies) and incubated 3 days at 37°C in humidified 5% CO2.
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Microbiology 2019Quote: Harvested human blood was incubated with RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies) or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... human PBMC (ST70025, STEMCELL Technologies) was mixed and incubated with MACS CD4 MicroBeads (130-045-101 ...
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bioRxiv - Cell Biology 2022Quote: ... IntestiCult™ Organoid Growth Medium (Human) or IntestiCult™ Organoid Differentiation Medium (Human) were used for culture or differentiation of human gut organoids (STEMCELL Technologies, Vancouver, Canada). Organoids were used prior to passage 3 for optimal efficiency of EEC and β-like cell induction.
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bioRxiv - Immunology 2020Quote: Primary human NK cells were enriched from the peripheral blood of human donors using RosetteSep Human NK cell Enrichment Cocktail (Stem Cell Technologies, Cat #15065) and cultured overnight in RPMI-1640 (Corning ...
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bioRxiv - Immunology 2020Quote: ... Two types of controls were used in all experiments except when noted: activated cells co-cultured in the presence of CD3/CD28/CD2 activating bead (STEMCELL Technologies, Vancouver, Canada) and non-activated cells co-cultured in the presence of complete media without beads ...
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bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Immunology 2021Quote: ... human neutrophils were purified from blood using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Human Monocyte were acquired from frozen PBMCs with STEMCELL EasySep Human Monocyte Enrichment kit (STEMCELL TECHNOLOGIES). Purified monocytes were cultured in RPMI-1640 Glutamax medium (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... 10μl Human LDL (StemCell Technologies, 02698) for every 50ml media and the following cytokines (all from MiltenyiBiotec unless stated otherwise) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.02% Human LDL (Stem Cell Technologies) and the following cytokines (Miltenyi Biotec unless stated otherwise) ...
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bioRxiv - Immunology 2020Quote: ... commercial cryo-human pDC (Stemcell Technology) from 5 healthy subjects ...
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bioRxiv - Bioengineering 2023Quote: ... Human recombinant VEGF-165 (Stemcell technologies; Saint Egrève ...
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bioRxiv - Immunology 2022Quote: B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15 ...
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bioRxiv - Immunology 2021Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were isolated using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human CD14+ monocytes were isolated from leukopaks using the EasySep Human CD14+ Positive Selection Kit (STEMCELL Technologies) and differentiated into monocyte-derived dendritic cells (moDCs ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
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bioRxiv - Cell Biology 2022Quote: ... human neutrophils were isolated from whole blood using the EasySep Direct Human Neutrophil Isolation kit from Stemcell Technologies by immunomagnetic negative selection ...