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Citations for Stemcell Technologies :
551 - 600 of 974 citations for Beta NGF Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4 T cells were purified using the naïve human CD4+ T cell isolation kit (StemCell Technology) and stimulated with immobilized anti-human CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were isolated from buffy coats by density gradient centrifugation (Lymphoprep®, Stemcell Technologies Cat# 07811) and cultured overnight in T-cell medium (CTS OpTmizer® ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were negatively purified with EasySepTM beads (human T-cell isolation kit, STEMCELL Technologies, Vancouver, Canada). T cells were cultured in Advanced RPMI 1640 enriched with FBS (10%) ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... medium was changed to SFEM II containing 10ng/mL recombinant human granulocyte colony stimulating factor (StemCell Technologies) and maintained in this medium until day 20 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cell Biology 2023Quote: H9 human embryonic stem cells (hESCs; female, WA09, WiCell) were cultured in mTeSR Plus medium (STEMCELL Technologies) with 1% Penicillin-Streptomycin in 8 mg/mL Matrigel-coated (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using EasySep Human Naïve CD4+ T Cell Isolation Kit (Stemcell technologies), according to manufacturer’s indications.
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Both epithelial and stromal cell pellets were then suspended in complete MammoCult human medium (STEMCELL Technologies, Inc., 05620) supplemented with hydrocortisone and heparin as per the manufacturer’s instructions and 1% pen/strep (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... The EasySep™ Direct Human Neutrophil Isolation Kit was used as per standard protocol (StemCell Technologies, cat. 19666). Isolation cocktail containing antibodies and RapidSpheres™ were added to the whole blood sample and immunomagnetic negative selection was performed ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: The EPO concentrations from 34 serum samples were determined using the Human Erythropoietin ELISA Kit from Stemcell Technologies. Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Cancer Biology 2021Quote: CD14+ monocytes were selected from frozen human PBMC using anti-CD14 microbeads according to manufacturer’s instructions (Stemcell Technologies). The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and in the presence of the commercially available medium IntestiCult™ Organoid Growth Medium (Human) (STEMCELL Technologies, 06010), supplemented with the antibiotic primocin (Invivogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and BT260 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05750) supplemented with EGF (20 ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...