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Citations for Stemcell Technologies :
101 - 150 of 168 citations for Anti TRIM15 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with specific neuronal marker CD24-FITC Clone M1/69 antibody(38) (Stemcell Technologies, Canada) diluted 1:100 for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... all from BD Biosciences; anti-CD38-FITC (clone AT1; 2.5 μL; cat. # 60131FI) from STEMCELL Technologies; anti-CD161-BV421 (clone HP-3G10 ...
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bioRxiv - Developmental Biology 2022Quote: ... Six-well plates were pre-treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... embryos upon depletion of erythroid cells using anti-Ter119 immunomagnetic microbeads (StemCell Technologies). Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with 1:25 anti-human CD45 FITC (clone 2D1, STEMCELL Technologies), 1:50 anti-human CD45 APC-ef780 (clone HI30 ...
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bioRxiv - Developmental Biology 2022Quote: ... Each well was precoated with 500µl of Anti-Adherence Rinsing Solution (Stemcell technologies) and spun for 5 minutes at 1500 rcf ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 cm dishes were coated with Anti-adherence rinsing solution (Stemcell technologies, 7010) during 2 min ...
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bioRxiv - Microbiology 2023Quote: ... Tissue culture dishes were treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies™). EBs were cultured in expansion medium until day 25 with medium changes every 2-3 days ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Antibody-bound cells were depleted with streptavidin RapidSphere™ magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies; Vancouver, Canada). Cells with >90% purity were used for experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Cell Biology 2021Quote: A volume of 50 µl of Anti-Adherence Rinsing Solution (STEMCELL Technologies, Cambridge, UK) was added to each well of a clear green coded 96-well round base plate for suspension (Sarstedt ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Immunology 2020Quote: ... were prepared by addition of 0.5 mL Anti-Adherence Rinsing solution (Stemcell technologies, #07010) and centrifugation at 3000g for 3 minutes to remove bubbles from the microwells ...
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bioRxiv - Neuroscience 2022Quote: ... The microwells were first coated with an anti-adherence rinsing solution (07010, StemCell Technologies) and centrifuged at 2,000 g for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 ml Falcon Tubes were rinsed with Anti-adherence Rinsing Solution (StemCell Technology, #07010) to prevent cell adhesion to the tube ...
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bioRxiv - Developmental Biology 2023Quote: ... 6-well plates were coated using an anti-adherence rinsing solution (Stem Cell Technologies) for 30min at 37℃ to make the low-attachment plates ...
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bioRxiv - Neuroscience 2023Quote: ... was prepared by centrifuging 500 µL Anti-Adherence Rinsing Solution (Stem Cell Technologies, 07010) at 1300 xg for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Immunology 2022Quote: ... was prepared by addition of 0.5ml of Anti-Adherence Rinsing Solution (STEMCELL Technologies, no 07010) and centrifuged at 3,000 x g for three minutes to remove bubbles from the microwells ...
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bioRxiv - Microbiology 2023Quote: The remaining 180 µL was stained with 20 µL of anti-APC beads (Stemcell technologies, EasySep APC Positive Selection Kit II ...
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bioRxiv - Neuroscience 2023Quote: ... that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies: 07010) for 5 mins before plating the cells ...
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bioRxiv - Genomics 2019Quote: ... and for 1 h at 4 0C with a combination of conjugated antibodies to surface proteins TRA-1-60 (StemCell Technologies, 60064PE), TRA-1-81(StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
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bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
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bioRxiv - Neuroscience 2023Quote: ... plated cells were immediately supplemented with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Cancer Biology 2023Quote: The purity of cultured CTC subpopulation was validated by gating the sorted cells with conjugated primary antibodies of CD44-FITC (Stem Cell Technologies), CD24-PE ...
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bioRxiv - Developmental Biology 2021Quote: A 100 μL volume of anti-adherence rinsing solution (StemCell Technologies, Vancouver, Canada, cat. no. 07010) was added to untreated sterile CellStar U-bottom or V-bottom (Figure 1D ...
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bioRxiv - Neuroscience 2024Quote: ... the microwells were coated with 500 µl of AggreWellTM Anti-adherence rinsing solution (7010, StemCell Technologies) and rinsed with 2 ml NeurobasalTM Plus (A3582901 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
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bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...
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bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was rinsed with 1x PBS and an anti-adherence rinsing solution (StemCell Technologies, Aggrewell™) was added into each well ...
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bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Cancer Biology 2021Quote: CD14+ monocytes were selected from frozen human PBMC using anti-CD14 microbeads according to manufacturer’s instructions (Stemcell Technologies). The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggrewell™400 was prepared by rinsing the wells with an anti-adherence rinsing solution (Stem cell technologies) as per the manufacturer’s protocol and washed with SL (without LIF ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized anti-coagulated blood using density gradient Lymphoprep medium (Stemcell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the mold was rinsed once with PBS and then coated with an anti- adherence rinsing solution (StemCell Technologies, # 07010) to increase the non-stick behavior of the PDMS further ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...