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1 - 50 of 221 citations for 9 3 bromophenyl 9 phenyl 9H fluorene since 2020

• Caveolin scaffolding domain (CSD) peptide LTI-2355 modulates the phagocytic and synthetic activity of lung derived myeloid cells in Idiopathic Pulmonary Fibrosis (IPF) and Post-acute sequelae of COVID-fibrosis (PASC-F)

  Brecht Creyns, et al., bioRxiv - Immunology 2024

  Quote: ... and DNaseI (9003-98-9, STEMCELL Technologies) for 40 min at 37°C in complete HBSS (21-023-CV ...
• Cardiomyocyte apoptosis contributes to contractile dysfunction in stem cell model of MYH7 E848G hypertrophic cardiomyopathy

  Alexander M. Loiben, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 9 μL Buffer R2 (STEMCELL Technologies, 100-0691), 1 μL 30 μM of gRNA (Table 2) ...
• Synthetic amyloid beta does not induce a robust transcriptional response in innate immune cell culture systems

  I.Y. Quiroga, et al., bioRxiv - Neuroscience 2021

  Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
• Elevated α-synuclein attenuates phagocytosis in SNCA triplication human iPSC-derived neuron:microglia co-cultures

  Richard Lieberman, et al., bioRxiv - Neuroscience 2022

  Quote: ... 9 x 106 cells were seeded into 6-well Aggrewell 800 plates (StemCell Technologies) in embryoid body media consisting of complete StemFlex supplemented with 10 μM Y27632 (Stemcell Technologies) ...
• Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis

  Dennis Ma, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Cells were harvested anytime between day 9-21 using dispase (StemCell Technologies, cat. no. 07913) and trypsin to dissociate Matrigel and spheres ...
• O-glycosylation contributes to mammalian glycoRNA biogenesis

  Jennifer Porat, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 500 µL thawed organoids were resuspended into 9 mL IntestiCult Human Organoid Growth Medium (Stemcell technologies) and centrifuged at 150 g at 4°C for 5 minutes ...
• An early cell shape transition drives evolutionary expansion of the human forebrain

  Silvia Benito-Kwiecinski, et al., bioRxiv - Evolutionary Biology 2020

  Quote: ... followed by expansion for 9 days in Expansion Media composed of Stemspan H3000 (Stem Cell Technologies, 09850) supplemented with 50μg/ml ascorbic acid (Sigma ...
• Serotonin attenuates tumor-necrosis-factor-alpha-induced intestinal inflammation by interacting with human mucosal tissue

  Veronika Bosáková, et al., bioRxiv - Immunology 2024

  Quote: ... DF19-9-7T (61)) were cultured in Matrigel-coated tissue culture dishes and maintained in mTeSR Plus medium (StemCell Technologies) in incubator at 37 °C ...
• Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme

  Andrea B. Alber, et al., bioRxiv - Developmental Biology 2022

  Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
• Broad de-regulated U2AF1 splicing is prognostic and augments leukemic transformation via protein arginine methyltransferase activation

  Meenakshi Venkatasubramanian, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Plates were kept in humidified chambers and colonies were imaged and manually scored after 9-14 days using the STEMvision counter (StemCell Technologies).
• A monoclonal antibody collection for C. difficile typing

  Lise Hunault, et al., bioRxiv - Immunology 2023

  Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, #TIB-9) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, #03800). Culture supernatants were screened using ELISA (see below ...
• Primate heart regeneration via migration and fibroblast repulsion by human heart progenitors

  Christine Schneider, et al., bioRxiv - Cell Biology 2020

  Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
• CARD8 inflammasome activation during HIV-1 cell-to-cell transmission

  Jessie Kulsuptrakul, et al., bioRxiv - Immunology 2024

  Quote: Primary monocytes were isolated via negative selection using the EasySep™ Human Monocyte Isolation Kit (Easy Sep, 1x10^9) (Stem Cell Technologies) according to the manufacturer’s protocols from PBMCs collected from healthy donors ...
• Flap-enabled next-generation capture (FENGC): precision targeted single-molecule profiling of epigenetic heterogeneity, chromatin dynamics, and genetic variation

  Mingqi Zhou, et al., bioRxiv - Cancer Biology 2022

  Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
• Gene expression and chromatin accessibility comparison in iPSC-derived microglia in African, European, and Amerindian genomes in Alzheimer’s patients and controls

  Sofia Moura, et al., bioRxiv - Genomics 2024

  Quote: ... HPCs were plated at 22,000 cells/cm2 onto a Matrigel-coated 6-well plate containing 2ml of Microglia differentiation medium (Basal Medium with supplement 1 and 2 at 1:9 and 1:225, respectively; STEMCELL Technologies, #100-0019). Cells were supplemented with fresh half-medium every other day from day 0 to day 10 ...
• Epitope Engineered Human Haematopoietic Stem Cells are Shielded from CD123-targeted Immunotherapy

  Romina Marone, et al., bioRxiv - Bioengineering 2023

  Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
• Human Cerebral Spheroids Undergo Activity Dependent Changes In Cellular Composition And Microrna Expression

  Thomas Parmentier, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
• Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors

  Joan Sebastián Gallego-Murillo, et al., bioRxiv - Bioengineering 2022

  Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
• Base-resolution models of transcription factor binding reveal soft motif syntax

  Žiga Avsec, et al., bioRxiv - Genomics 2020

  Quote: ... 3 µM CHIR99021 (Stemcell Technologies), 1 µM PD0325901 (Stemcell Technologies) ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 3 µM CHIR9902 (Stemcell Technologies) + 10 µM SB-431542 (Stemcell Technologies ...
• Building functional circuits in multispecies brains

  Benjamin T. Throesch, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 3 μM CHIR99021 (STEMCELL Technologies), 1 μM PD0325901 (STEMCELL Technologies) ...
• Antisense oligonucleotide targeting pathogenic sense repeat RNA in C9ORF72 suppresses production of antisense-dependent dipeptide repeat proteins implicated in ALS/FTD

  Yuanzheng Gu, et al., bioRxiv - Neuroscience 2024

  Quote: ... 3 μM CHIR99021 (StemCell Technologies), 2 μM DMH1 (StemCell Technologies) ...
• Use of Induced Pluripotent Stem Cells to Build Isogenic Systems and Investigate Type 1 Diabetes

  Lucas H. Armitage, et al., bioRxiv - Immunology 2021

  Quote: ... and 3 mM CHIR (StemCell Technologies)) ...
• Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm

  Dominika Dziedzicka, et al., bioRxiv - Cell Biology 2020

  Quote: ... 3 µM CHIR99021 (CHIR, STEMCELL Technologies) and 100 ng/mL Activin A (Biotechne) ...
• Circadian regulation of lung repair and regeneration

  Amruta Naik, et al., bioRxiv - Cell Biology 2021

  Quote: ... CHIR99021 3 µm (72052 StemCell Technologies), DMSO (final concentration 0.05% ...
• Chromatin Profiling of CBFA2T3-GLIS2 AMLs Identifies Key Transcription Factor Dependencies and BRG1 Inhibition as a Novel Therapeutic Strategy

  Samantha Kaonis, et al., bioRxiv - Cancer Biology 2023

  Quote: ... + 10ng/ml IL-3 (STEMCELL Technologies). The WSU-AML cell line was also seeded at 1x106 cells/ml ...
• Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs

  A. Lorzadeh, et al., bioRxiv - Cell Biology 2020

  Quote: ... and 3 units/mL EPO (STEMCELL Technologies) in the presence of 1 µM GSK-J4 (Sigma ...
• Enhanced donor heart preservation by therapeutic inhibition of acid sensing ion channel 1a

  Meredith A. Redd, et al., bioRxiv - Physiology 2020

  Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ...
• Human bone marrow organoids for disease modelling, discovery and validation of therapeutic targets in hematological malignancies

  Abdullah O. Khan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Interleukin-3 (IL3, StemCell Technologies, Cat#78194) and Interleukin-6 (IL6 ...
• Structurally and functionally distinct early antibody responses predict COVID-19 disease trajectory and mRNA vaccine response

  Saborni Chakraborty, et al., bioRxiv - Immunology 2021

  Quote: ... anti-CD32 (STEMCELL Technologies; clone IV.3), anti-CD32B/C (clone S18005H) ...
• IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition

  Caterina Prelli Bozzo, et al., bioRxiv - Microbiology 2020

  Quote: ... CHIR 99021 3 µM (Stemcell Technologies, 72054), Pumorphamine 1 µM (Miltenyi Biotec ...
• Modeling lung cell development using human pluripotent stem cells

  Shuk Yee Ngan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 3 µM CHIR99021 (STEMCELL Technologies, cat#72054), 100 nM All-trans Retinoic acid (ATRA ...
• Cell cycle gene regulation dynamics revealed by RNA velocity and deep-learning

  Andrea Riba, et al., bioRxiv - Systems Biology 2021

  Quote: ... 3 µM CHIR99021 (72054, Stem Cell Technologies) and 1 µM PD0325901 (72184 ...
• Reticular Dysgenesis-associated Adenylate Kinase 2 deficiency causes failure of myelopoiesis through disordered purine metabolism

  Wenqing Wang, et al., bioRxiv - Cell Biology 2021

  Quote: ... 100μg/mL hrIL-3 (StemCell Technologies 78040), 10μg/mL hrG-CSF (NEUPOGEN ...
• Soluble NS1 antagonizes IgG- and IgA-mediated monocytic phagocytosis of DENV infected cells

  Mitchell J. Waldran, et al., bioRxiv - Immunology 2022

  Quote: ... -32 (Stemcell Technologies 60012 clone IV.3), or -64 (Biolegend 305002 Clone 10.1).
• MAPT expression is mediated by long-range interactions with cis-regulatory elements

  Brianne B. Rogers, et al., bioRxiv - Genomics 2023

  Quote: ... 10 µg/mL NT-3 (StemCell Technologies), and 1 mg/mL Mouse Laminin (Gibco) ...
• Differentiation of airway cholinergic neurons from human pluripotent stem cells for airway neurobiology studies

  P.A. Goldsteen, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 3 µM CHIR99021 (STEMCELL Technologies, 72054), was freshly added ...
• Engineering human spinal microphysiological systems to model opioid-induced tolerance

  Hongwei Cai, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 3 μM CHIR-99021 (Stemcell Technologies). After 4-day culture in SCM I ...
• Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

  Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

  Quote: ... CHIR99021 (3 μM, STEMCELL Technologies, Cat#72052), purmorphamine (0.5 mM ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 3 U/mL heparin (StemCell Technologies) for differentiating progenitor cells into erythrocytes.
• GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis

  Joseph A Bisson, et al., bioRxiv - Developmental Biology 2024

  Quote: ... 3 μM CHIR99021 (Stem Cell Technologies 72054) was included in the “Day 0 medium” and/or the concentration of BMP4 was reduced to 5 ng/mL (Figure 6C-E ...
• Fetal Liver-like Organoids Recapitulate Blood-Liver Niche Development and Multipotent Hematopoiesis from Human Pluripotent Stem Cells

  Milad Rezvani, et al., bioRxiv - Developmental Biology 2024

  Quote: ... 50 ng/ml IL-3 (STEMCELL Technologies), 50 ng/ml IL-34 (Biolegend) ...
• Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

  Frøydis Sved Skottvoll, et al., bioRxiv - Biochemistry 2020

  Quote: ... containing 3 μM CHIR99021 (STEMCELL Technologies, Vancouver, Canada), 50 nM PI-103 from Bio-Techne Ltd ...
• Leveraging complex interactions between signaling pathways involved in liver development to robustly improve the maturity and yield of pluripotent stem cell-derived hepatocytes

  Claudia Raggi, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and 3 μM CHIR-99021 (Stem Cell Technologies), and then for the 3 following days to 100 ng/ml Activin A alone ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... Human Peripheral Blood Plasma (3%; Stem Cell Technologies), 3,3‘,5-Triiodo-L-thyronine (1 µM ...
• High-content fluorescence bioassay to investigate pore formation, ion channel modulation and cell membrane lysis induced by venoms

  Simon Kramer, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies, Canada), 500 μg.mL-1 bovine serum albumin (BSA ...
• Differentiation of airway cholinergic neurons from human pluripotent stem cells for airway neurobiology studies

  P.A. Goldsteen, et al., bioRxiv - Neuroscience 2022

  Quote: ... 3 µM CHIR99021 (STEMCELL Technologies, Vancouver, Canada, 72054), and 1 µM retinoic acid ...
• Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations

  Vincent Soubannier, et al., bioRxiv - Neuroscience 2023

  Quote: ... 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
• A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleation

  Arit Ghosh, et al., bioRxiv - Cell Biology 2024

  Quote: ... 1 ng/mL IL-3 (STEMCELL Technologies, 78040) and 1% Penicillin-Streptomycin (Gibco 15140122) ...
• DDX41 dissolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death

  Honghao Bi, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and Interleukin-3 (IL3, StemCell Technologies, Cat#78194) and Interleukin-6 (IL6 ...