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Citations for Stemcell Technologies :
501 - 550 of 1146 citations for 7 cyano 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Neuroscience 2022Quote: ... were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560) on Corning® Matrigel® hESC-qualified Matrix (Corning cat#354277 ...
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bioRxiv - Cell Biology 2020Quote: ... C3H10T1/2 cells and MSCs were treated with Mesencult ™ Osteogenic Stimulatory Kit (STEMCELL TECHNOLOGIES, #05504).
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day the medium was replaced with STEMdiff™ APEL™2 (StemCell Technologies, #05270), containing 8 μM CHIR-99021 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Microbiology 2021Quote: ... A nasal brushing of the turbinate was acquired using 3-mm bronchial cytology brush and placing the biopsy into warm PneumaCult-Ex Plus Medium (STEMCELL Technologies, Cambridge, UK). The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... with 1% PS and 1 μg/mL doxycycline (StemCell Technologies 72742). Prior to orthotopic injection of primary PDAC cell lines ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transferred into TeSR-E7 medium and maintained until the emergence of iPSC colonies (about 4 weeks) which were then manually isolated and propagated in mTeSR medium (StemCell Technologies). Absence of plasmid integration and clearance of Sendai virus were confirmed ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with phosphate-buffered saline (PBS) (5 ml) and detached by addition of ‘Gentle Dissociation Reagent’ (3 ml) (STEMCELL Technologies; Cat. No. 07174) stored at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were dissociated and plated at a density of 3×104 cells/cm in STEMdiff™ Neuron Maturation Kit (Stem Cell Technologies, #08510) medium on a double layer of poly-L-ornithine (Sigma-Aldrich #P3655 ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 2 min in NH4Cl red blood cell lysis solution (StemCell Technologies, cat. # 07800, Vancouver, Canada), and seeded into culture plates in αMEM supplemented with 15% FBS and antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
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bioRxiv - Physiology 2022Quote: ... then medium changed to 1:1 cFAD+RI:Pneumacult ALI (Stemcell Technologies, UK) for 2 days to ensure monolayers were 100% confluent ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...