Labshake search
Citations for Stemcell Technologies :
151 - 200 of 985 citations for 7 TRIFLUOROMETHYL 2 3 4 5 TETRAHYDRO 1H BENZO B AZEPINE HYDROCHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
-
bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
-
bioRxiv - Immunology 2024Quote: B cells were isolated from blood of convalescent COVID-19 donors using the EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech ...
-
bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
-
bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
-
bioRxiv - Cell Biology 2019Quote: ... Human neutrophils were isolated within 1h after drawn using the human neutrophil direct isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
-
bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
-
bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
-
bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
-
bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
-
bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
-
bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
-
bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
-
bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
-
bioRxiv - Microbiology 2019Quote: ... or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies) and then isolated with Ficoll-Paque Plus (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary cells were resuspended in Epicult-B (Stemcell Technologies) media containing 2% Matrigel and 12,000 cells were seeded into each chamber in a total volume of 400 μL ...
-
bioRxiv - Cancer Biology 2021Quote: ... and immersed in EpiCult-B Medium (STEMCELL Technologies, 05610) supplemented with 10 ng/mL human recombinant EGF (PeproTech ...
-
bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
-
bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... supplemented with 7 μM CHIR 99021(STEMCELL Technologies, Catalog #72052) for 3 days ...
-
bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
-
bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
-
bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
-
bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
-
bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
-
bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
-
bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were replated in prepared Medium B from Stemcell technologies. The manufacturer’s protocol was followed for an additional 7-10 days and numerous CD43+ progenitor cells (HPC ...
-
bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
-
bioRxiv - Genetics 2020Quote: ... to each well for 1h at room temperature) containing 0.5ml complete ReproTeSR medium (ReproTeSR basal medium with 25X ReproTeSR supplement) (Stem Cell Technologies). After 24h incubation ...
-
bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
-
Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
-
bioRxiv - Microbiology 2020Quote: ... Primary human B cells were isolated by negative selection using RosetteSep Human B Cell Enrichment and EasySep Human B cell enrichment kits (Stem Cell Technologies, #15064 and #19054), according to the manufacturers’ protocols ...
-
bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
-
bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
-
bioRxiv - Neuroscience 2023Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
-
bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...