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Citations for Stemcell Technologies :
251 - 300 of 669 citations for 7 Chloro 1H pyrrolo 3 2 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: AML3 - 7 and PBMC3 were thawed and kept in culture for 16-24 hours in Stem Span H3000 media (STEMCELL Technologies) supplemented with 50 μg/ml ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... DCs and naïve CD4+ T cells were harvested from SPLNs of mice at 7 weeks of age using a CD11c positive selection kit II (STEMCELL Technologies) and a mouse naïve CD4+ T cell Isolation kit (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μg/ml Heparin (STEMCELL Technologies), 20 ng/ml bFGF (Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/ml heparin (StemCell Technologies), epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2020Quote: ... 2% NeuroCult SM1 supplement (STEMCELL Technologies), 2 mM GlutaMax.
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/mL FGF-2 (StemCell Technology), and 5nM Rock inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µM SB431542 (StemCell Technologies). Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2% SM1 (Stemcell Technologies, Vancouver, Canada), and 5 μM cytosine β-d-arabinofuranoside (AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg/ml heparin (Stemcell Technologies), 50 units/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... B27 (2%v/v, Stemcell technologies), N2 (0.5%v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult SM1 (05711, Stemcell technologies), 1% N2 Supplement (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... in the same 3:4 mixture of media (StemCell Technologies #05620) and Matrigel (BD Bioscience CB-40324) ...
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bioRxiv - Cell Biology 2021Quote: ... and IL-3 (1ng/mL first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL (CASY Model TCC ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Immunology 2020Quote: ... Following enumeration using 3% acetic acid with methylene blue (StemCell Technologies), splenocytes were resuspended in phosphate-buffered saline and forty to sixty million cells were injected either intraperitoneally or intravenously (via retro-orbital injection under isoflurane anesthesia ...
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bioRxiv - Neuroscience 2022Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies) until neural induction ...
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bioRxiv - Neuroscience 2022Quote: ... and passaged every 3-4 days using ReLeSR (Stem Cell Technologies). Human iPSCs were edited for homozygous knockout of TAOK2 or heterozygous knock-in of the A135P mutation as described in Deneault et al.(Deneault et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 µM of CHIR99021 (GSK3B inhibitor, STEMCELL Technologies, cat # 72054). From day 1 to 3 ENR was supplemented with 20% Wnt3a-CM and 10 μM Y-27632.
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bioRxiv - Neuroscience 2022Quote: ... 3×106 single-cell suspension (Gentle Cell Dissociation Reagent, StemCell Technologies) of iPSCs were plated ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Developmental Biology 2024Quote: ... in motor neuron differentiation medium with 3 uM CHIR99021 (Stemcell Technologies) and cultured for 2 days to form neurospheres ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... Untreated peripheral blood B cells were isolated from the PBMCs of healthy donors by negative selection with the RosetteSep for isolation of B cells (StemCell Technologies). All samples were used when they contained ≥95% CD19+ cells ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Immunology 2022Quote: ... and M2A-GFP KI mice (gift of R. Adelstein, NHLBI/NIH) of either sex using negative selection B cell isolation (StemCell Technologies). Euthanasia was performed in accordance with protocols approved by the National Human Genome Research Institute Animal Use and Care Committee at the National Institutes of Health ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Spleens were macerated through a 70µm mesh filter and cells were separated using the EasySep Mouse B-cell separation kit (StemCell Technologies 19854A). Cells were then treated in B-cell culture media made using 438 mL RPMI 1640 (Gibco 11835-030) ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).