Labshake search
Citations for Stemcell Technologies :
101 - 150 of 1406 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 4 to 5 days using ReLeSR (StemCell Technologies, catalog no. 05872). We performed characterization of our iPSCs by immunofluorescence ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 μM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 µM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
-
bioRxiv - Cell Biology 2020Quote: ... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
-
bioRxiv - Developmental Biology 2021Quote: ... coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2) in mTeSR1 (StemCell Technologies). These were passaged every 4-6 days with Accutase (#A6964 ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
-
bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 5 mg ml-1 dispase (STEMCELL Technologies) and 1 mg ml-1 DNAse (Sigma ...
-
bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
-
bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... Common Rule (Category 4) or 2) de-identified donor leukapheresis products obtained from StemCell Technologies. CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
-
bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
-
bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
-
bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).
-
bioRxiv - Cancer Biology 2022Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). In the morning ...
-
bioRxiv - Cell Biology 2021Quote: ... This was followed by cardiac specification using RPMI B27-containing 5 μM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Cell Biology 2023Quote: ... This was followed by cardiac specification using RPMI B27-containing 5 μM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). On the morning ...
-
bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
-
bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
-
bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
-
bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 5 μl DNase (1 mg/ml Stemcell Technologies) at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... were passaged 1:4 using ACCUTASE™ (Stemcell technologies, 07920) when confluent and subsequently grown in Astrocyte medium containing leukemia inhibitory factor (LIF ...
-
bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
-
bioRxiv - Cell Biology 2019Quote: ... with a full medium change at day 2 and passage at day 3 using Accutase (STEMCELL Technologies, 07920).
-
bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
-
bioRxiv - Neuroscience 2019Quote: iPSCs were cultured at 37°C and 5% CO2 in a humidified incubator in mTesR1 medium (STEMCELL Technologies, #85850) on 6-well plates (Corning ...
-
bioRxiv - Bioengineering 2022Quote: iPSC lines were maintained and expanded at 37°C in 5% CO2 in mTeSR™Plus medium (Stemcell Technologies) on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
-
bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
-
Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
-
bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
-
bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
-
bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
-
bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
-
bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
-
bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
-
bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
-
bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).