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Citations for Stemcell Technologies :
301 - 350 of 1072 citations for 7 CHLORO 1 METHYL 1H PYRAZOLO 4 3 B PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Spleens were macerated through a 70µm mesh filter and cells were separated using the EasySep Mouse B-cell separation kit (StemCell Technologies 19854A). Cells were then treated in B-cell culture media made using 438 mL RPMI 1640 (Gibco 11835-030) ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Systems Biology 2020Quote: ... APC-CD49f (4 µL; Stem Cell Technologies 10109, clone VU1D9), Biotin-CD2 (8 µL ...
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bioRxiv - Neuroscience 2022Quote: ... Colonies were passaged every 4 days with ReLeSR (Stemcell technologies) after rinsing with Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cell Biology 2021Quote: IMR90-4 iPSCs (WiCell) were cultured in mTesR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... 4*106 iPSCs were seeded per Aggrewell 800 (Stemcell Technologies) in a 24-well plate in 2 ml embryonic body medium (EBM) ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Developmental Biology 2021Quote: ... 5% CO2 for 14 days and the CFC content was automatedly imaged after 7 and 14 days by using the StemVision imaging system (Stem Cell Technologies, Version 2.0.1.0). Additionally ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... This medium was supplemented with 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 cells were then plated onto microwells (AggreWell 800, STEMCELL technologies) for embryoid body induction ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Physiology 2020Quote: ... Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies). HIOs were generated according to protocols established in our lab (Múnera and Wells ...
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bioRxiv - Microbiology 2023Quote: ... and 4 ug/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µg/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Microbiology 2023Quote: ... and 4 ug/mL Heparin sodium salt in PBS (StemCell Technologies) was replaced on the basolateral side only ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were split every 4 days using ReLeSR (STEMCELL Technologies Inc.) and plated 1×106 and 2×106 cells on Geltrex (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4 days using ReLeSR media (StemCell Technologies) and were then seeded on a six-well Matrigel-coated plate at a density of 175,000 cells cm−2 in mTeSR-Plus medium ...
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bioRxiv - Cell Biology 2023Quote: ... Heparin 4 µg/mL (Ref 07980, Stemcell technologies, Saint Égrève, France) and Hydrocortisone 480 ng/mL (Ref 07925 ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Bioengineering 2019Quote: ... SVF was plated at a cell density of 7×106 in T75 tissue culture flasks in 15 ml of MesenCult MSC basal medium (Stem Cell Technologies, Cambridge, MA, USA) supplemented with MesenCult MSC Stimulatory Supplement ...