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Citations for Stemcell Technologies :
101 - 150 of 293 citations for 7 8 Dihydro 6 5H quinolinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: B cells subsets were obtained from WT or IL17 −/− naïve mice or from mice 14 d after alloantigen immunization (2 × 10 7 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Immunology 2021Quote: ... iPSC were maintained on Matrigel-coated 6 well plates in mTeSR+ medium (StemCell Technologies). Differentiation to sBC was carried out in suspension□based ...
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bioRxiv - Bioengineering 2022Quote: ... wells of a 6-well microwell culture plate (AggreWell™400, STEMCELL Technologies, USA) were replicated out of Hydrosil (101301 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 9 x 106 cells were seeded into 6-well Aggrewell 800 plates (StemCell Technologies) in embryoid body media consisting of complete StemFlex supplemented with 10 μM Y27632 (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were passaged every 5-6 days using ReLeSR dissociation reagent (STEMCELL Technologies, 05872) and used for hematopoietic stem cell differentiation with STEMdiff Hematopoietic kit (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C and passaged every 5-6 days using ReLeSR (Stem Cell Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... 6-well plates were coated using an anti-adherence rinsing solution (Stem Cell Technologies) for 30min at 37℃ to make the low-attachment plates ...
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bioRxiv - Cell Biology 2023Quote: ... On days 4 and 6 LDM medium with added 50ng/ml BMP4 (STEMCELL Technologies) and 6ul/ml DMSO was used ...
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bioRxiv - Biochemistry 2022Quote: ... cardioids were maintained in CDM with insulin.21,31 Samples were collected 7 days post induction and dissociated using the STEMdiff cardiomyocyte dissociation kit (#05025, Stem Cell Technologies) and subsequently washed 3x with PBS.
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bioRxiv - Bioengineering 2020Quote: ... After 72 hours the medium was replaced with 7 mL of neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 nM and smoothened agonist (SAG ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 μM Y-23632 (only for the initial 24 hours; Wako) and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies).
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bioRxiv - Cell Biology 2021Quote: AML3 - 7 and PBMC3 were thawed and kept in culture for 16-24 hours in Stem Span H3000 media (STEMCELL Technologies) supplemented with 50 μg/ml ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... DCs and naïve CD4+ T cells were harvested from SPLNs of mice at 7 weeks of age using a CD11c positive selection kit II (STEMCELL Technologies) and a mouse naïve CD4+ T cell Isolation kit (STEMCELL Technologies) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were seeded at a 1:6 ratio and routinely passaged with Dispase (Stemcell Technologies).
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bioRxiv - Biochemistry 2022Quote: ... hiPSCs were seeded on Matrigel-coated 6-well plates with mTeSR plus medium (STEMCELL Technologies) and 10 μM Y27632 ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Immunology 2021Quote: ... 6 tissue biopsies from each endoscopic region were placed in HypoThermosol medium (Stemcell Technologies 07935) and were transported on ice to the laboratory for further processing.
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bioRxiv - Immunology 2023Quote: ... CD11c+ cells were isolated from the spleens of C57BL/6 by positive selection (Stemcell Technologies) to serve as antigen presenting cells ...
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bioRxiv - Developmental Biology 2022Quote: Cardioids (8 cardioids per condition) were dissociated using a 1.5 mL CM dissociation medium (Stem Cell Technologies, #05025) for 7 - 10 min at 37°C ...
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bioRxiv - Genetics 2023Quote: ... 1 million cells were cultured for 8 days in StemSpanTM SFEM II medium (Stem Cell Technologies Catalog #09605) supplemented with 1X StemSpanTM Erythroid Expansion Supplement (Stem Cell Technologies Catalog #02692).
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Donor 2395 iPSC were maintained on Matrigel-coated 6 well plates in mTeSR+ medium (StemCell Technologies) and differentiated to sBC as previously described with some modifications (29) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Bioengineering 2019Quote: ... onto 6-well plates coated with 1mL/well of 1µg/cm2 Vitronectin XF (STEMCELL Technologies 07180) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Bioengineering 2022Quote: ... coated 6 well plates were used to culture WA09 cells with mTESR plus medium (Stemcell Technologies) in an incubator at 37 degrees Celsius and 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... rehydrated tissue was placed into a well of a 6-well plate (Stem Cell Technologies #38015) with 1 mL of ice-cold CST lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Neuroscience 2023Quote: ... and plated into a single well of a 6-well Aggrewell plate (Stemcell Technologies Aggrewell 800) that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from whole blood within 8 h of sample collection by using SepMate-15 tubes (STEMCELL Technologies) with Ficoll-Paque Plus (GE Healthcare Life Sciences ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregates were cultured from day 8 to day 10 in A-RPMI or TeSR™-E6 (StemCell Technologies 05946) medium supplemented with 25% M.CM ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2020Quote: ... Peripheral blood mononuclear cells were cultured for 8 days in erythroblast expansion medium (StemSpan™ SFEM medium (Stem Cell Technologies) supplemented with Epo (2 U/mL) ...