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Citations for Stemcell Technologies :
151 - 200 of 209 citations for 6 n Hexylaminopurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... 2.5 g of Hydrosil silicone components (1:1 wt) (101301; SILADENT) was added into each well of 6-well microwell culture plate (AggreWellTM400; STEMCELL Technologies) followed by centrifugation at 55 x g for 1 min and curing at 60 °C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Neuroscience 2024Quote: ... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Bioengineering 2023Quote: ... gift from the Seidman Lab) or GFP-DSP reporter (WTC; Allen Institute AICS-0017 cl.6) were cultured in mTeSR1 media (StemCell Technologies) on Matrigel (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
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bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... all the cells transfected with Sendai virus were transferred into a 6-well plate (30,000 cells per well) each well containing ~150,000 mouse embryonic fibroblast (MEF) feeder cells (Stemcell Technologies Inc. Vancouver, Canada). The MEF plate preparation was conducted the day before plating the reprogramming cells as explained elsewhere (28) ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 07180)-coated tissue culture plates and split in a ratio of 1:6 to 1:12 every four to six days with Gentle Cell Dissociation Reagent (STEMCELL Technologies, 07174). Alveolar differentiation was induced as previously described (Riva et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated into single cells and seeded onto matrigel coated plates at a density of 400-600k cells per well of a 6 well plate in mTeSR (Stem Cell Technologies) containing 10 µM Rock inhibitor (Y27632 ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...