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Citations for Stemcell Technologies :
301 - 350 of 549 citations for 6 ethoxybenzo b thiophen 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... rehydrated tissue was placed into a well of a 6-well plate (Stem Cell Technologies #38015) with 1 mL of ice-cold CST lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... responder CD4+ T cells were isolated from OTII BL/6 mice by negative selection (STEMCELL Technologies). Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Neuroscience 2023Quote: ... and plated into a single well of a 6-well Aggrewell plate (Stemcell Technologies Aggrewell 800) that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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bioRxiv - Genetics 2019Quote: ... Following six PBS platelet removal washes these were resuspended at 1×106 cells/ml in phase one media: Stemspan medium (Stemcell Tech), 1μg /ml cyclosporin A (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons differentiated with forebrain media were kept in differentiation media-STEMdiff forebrain neuron differentiation medium + supplements for one week and subsequently in maturation media – Brainphys Neuronal Medium + supplements (STEMCELL technologies) for the remaining days in culture ...
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bioRxiv - Cell Biology 2022Quote: Two different culture media were tested for hIO growth and differentiation: one commercial (“SCT-ODM”: IntestiCult Organoid Differentiation Medium, StemCell Technologies) and one homemade cocktail (“CCM” ...
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bioRxiv - Developmental Biology 2024Quote: ... A 1-5 min centrifugation of the well-plate was performed at 200g and cells in suspension were cultured for one day in mTesR Plus (StemCell Technologies) to allow for aggregation and Embryoid Body (EB ...
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bioRxiv - Developmental Biology 2021Quote: Unsorted cells at day 6 (4+2) of differentiation were cultured in MethoCult 04434 (Stem Cell Technologies) in 35 mm dishes ...
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bioRxiv - Cell Biology 2022Quote: ... medium was replenished daily and were passaged every 5-6 days with Dispase I (StemCell Technologies, 07923) in small cell clumps ...
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bioRxiv - Genomics 2021Quote: ... in a well of a Matrigel-coated 6 well plate supplemented with 10μM Y27632 (Stem Cell technologies).
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bioRxiv - Cell Biology 2022Quote: ... and WTC-11 lines and cultured in 6-well plates were dissociated with TRPYLE (Stem Cell Technology), centrifuged at 300 g ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies), irradiated (20 Gy ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.1 mL was plated per well in a SmartDish 6-well plate (27371, STEMCELL Technologies, USA) in duplicates ...
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bioRxiv - Bioengineering 2023Quote: ... on Matrigel-coated 6-well plate and passaged 1:12 with ReLeSR™ (Catalog # 05872, STEMCELL Technologies) every four days.
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bioRxiv - Bioengineering 2023Quote: ... monocytes were seeded into a 6-well plate in ImmunoCult™-ACF Dendritic Cell Medium (Stemcell Technologies), supplemented with recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2024Quote: ... seeded 18 hours prior in a 6-well plate with 1 mL of mTESR1 (StemCell Technologies 85850) or MEM medium ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: ... and 8 weeks old adult mammary glands (#4 gland only) were dissected and pooled from multiple animals into dissection media (Epicult-B Basal medium (Stem Cell Technologies #05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Passage one cells were seeded into T75 cell culture flasks (250,000 cells/flask) and expanded in PneumaCult ExPlus medium (StemCell Technologies, Vancouver, Canada) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Bioengineering 2021Quote: ... single cells suspended in a 3:4 mixture of Mammocult (StemCell Technologies 05620) and Matrigel (Corning 354234 ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... This medium was supplemented with 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 cells were then plated onto microwells (AggreWell 800, STEMCELL technologies) for embryoid body induction ...
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bioRxiv - Systems Biology 2023Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
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bioRxiv - Systems Biology 2023Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Neuroscience 2020Quote: ... hiPSC colonies were passaged 1:6 every 4 to 7 days with Gentle Cell Dissociation Reagent (STEMCELL Technologies). Genomic integrity was validated by G-band karyotyping (Cell Line Genetics ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were maintained on ESC-qualified Matrigel-coated 6-well plates in mTeSR plus medium (STEMCELL Technologies) with 10 μM Y-23632 (only for the initial 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the EB were transferred to a 6-well ultra-low adherent plate with Expansion Medium (Stem Cell Technologies). After 3 days ...
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bioRxiv - Immunology 2022Quote: ... 1.1ml of the mixture was slowly distributed to a well in 6-well SmartDishTM plates (StemCell Technologies, 27370). 6-well plates were incubated in a 37°C ...