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Citations for Stemcell Technologies :
551 - 600 of 610 citations for 6 Trifluoromethyl quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2022Quote: ... then gently detached with Accutase and reseeded as 2-5×104 cells/cm2 in mTeSR medium containing 10uM Y-27632 (#72304, STEMCELL Technologies) ROCK inhibitor (removed after 48h and exchanged for fresh medium without ROCK inhibitor) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cell Biology 2023Quote: ... 2×105 freshly isolated cells from digests of aorta or specified tissue were plated in methylcellulose (MethoCult GF M3434, StemCell Technologies), in duplicate ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2024Quote: Whole blood was collected from 12M and 20M SM/J and BL6 mice via intracardiac puncture and diluted into an equal volume of Dulbecco’s PBS + 2% FBS (StemCell Technologies, 07905). According to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... medium was refreshed weekly by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies) and 1% pen/strep (BP-complete medium).
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bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Bioengineering 2021Quote: hiPSC-derived endothelial cells (hECs) were differentiated as previously described.[85] hiPSCs were subjected to 5 μM CHIR99021 in STEMdiff APEL 2 Medium (STEMCELL Technologies, 05270) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were then collected and spun down at 500rpm for 1-2 minutes and resuspended in 2mL of mTeSR Plus media with 20 µM ROCK inhibitor Y-27632 (Stemcell Technologies # 72304). Matrigel ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Immunology 2022Quote: The collected blood was 1:1 diluted with 2% FBS and was added to 7ml of Lymphoprep™ density gradient medium in SepMate-15 tubes (StemCell Technologies). The red blood cells ...
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bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples were extracted from differentiated NPCs or undifferentiated NPCs treated for 2 hrs with STEMdiff™ Forebrain Neuron Differentiation medium (Stem Cell Technologies) for differentiated NPCs ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...