Labshake search
Citations for Stemcell Technologies :
501 - 550 of 856 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were purified from CD45.1+ CD45.2+ or CD45.2+ SMARTA splenocytes by negative selection (STEMCELL Technologies) and stimulated in 12-well plates pre-coated with 8 μg/mL anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
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bioRxiv - Immunology 2020Quote: Naïve CD4+ T cells were negatively isolated from fresh PBMCs by EasySep (Stem Cell Technologies; >95% purity). Cells were stimulated (Ellis et al. ...
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bioRxiv - Immunology 2020Quote: ... or αCD3/CD28 per manufacturer’s instructions (ImmuCult human CD3/CD28 T cell activator; StemCell Technologies, Cambridge, MA). After stimulation ...
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bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
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bioRxiv - Genomics 2021Quote: ... Frozen vials of primary human CD4+CD45RO+ memory T cells were purchased from STEMCELL Technologies (Cat #70031).
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bioRxiv - Immunology 2020Quote: ... Control T cells were isolated from from leukapheresis products from healthy donors (STEMCELL Technologies, Vancouver, BC, Canada). This study was approved by the Research Ethics Board of the BC Cancer Agency/University of British Columbia (certificates H16-00291-A021 ...
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bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2021Quote: Naive P14 CD8+ T cells from wildtype mice were isolated by negative selection (EasySep™, Stemcell Technologies). Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2021Quote: Primary human CD3+ T cells were isolated from donor PBMCs via immunomagnetic negative selection (Stem Cell Tech). For thaw and culture ...
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bioRxiv - Immunology 2023Quote: ... Lymph node CD8α+ T cells were isolated and sorted using the CD8α Positive Selection Kit (Stemcell Technologies). Isolated cells were washed and resuspended at 1 x 106 cells/ml in stimulation media ...
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bioRxiv - Immunology 2023Quote: ... Tissue was collected in ImmunoCult-XF T cell Expansion Medium (immunoCult-XF; STEMCELL Technologies, Vancouver, BC, Canada) with 1% penicillin/streptomycin (P/S ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Synthetic Biology 2023Quote: ... and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (25 µL/mL) (Stemcell Technologies, Cat.#10990) and cultured for 1.5 days at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml mIL3 and 20 ng/ml mSCF (STEMCELL Technologies, Vancouver, Canada) at a concentration of 500 or 1,000 cells per ml ...
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bioRxiv - Developmental Biology 2022Quote: ... on a 6-well plate previously coated with Vitronectin XF (StemCell Technologies, #7180). After two days ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were passaged on day 6 using Accutase (#07920, Stemcell Technologies, Canada) and transferred to a new PLO-laminin-coated 6-well plate with a cell density of 1.5*106 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from C57BL/6 mice were digested with collagenase/Hyaluronidase (Stemcell Technologies, #07912) and DNase1 solution (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Bioengineering 2024Quote: ... Cultures were then maintained for 6 weeks in MyeloCult™ (StemCell Technologies, 05150) supplemented with 1x10-6 M hydrocortisone (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4 T cells were isolated from CB mononuclear cells (CBMCs) obtained using Lymphoprep (StemCell Technologies Inc., Canada), followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Bioengineering 2020Quote: ... as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies) T cell enrichment as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... counted and CD8+ or CD4+ T cells were magnetically separated with appropriate separation kits (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified by negative selection using the EasySep™ CD4 enrichment kit (Stem cell Technologies), and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Microbiology 2021Quote: ... CD14+ monocytes and CD4+ T-cells were purified from PBMCs by negative magnetic selection (Stemcell Technologies, Grenoble, France). Monocytes (1×106/well ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ cells were enriched from human PBMCs with EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Immunology 2023Quote: Splenic B cells or T cells were Isolated with EasySepTM Mouse B Cell Isolation Kit (#19854, StemCell Technologies) or EasySepTM Mouse T Cell Isolation Kit (#19851 ...
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bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Murine CD4 T cells were isolated from processed lymphoid organs using EasyEights magnet (Stem Cell Technologies, Catalog no. 18103) and the whole CD4+ T cell isolation kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.