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Citations for Stemcell Technologies :
401 - 450 of 517 citations for 6 TERT BUTYLCHROMONE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Both UC3-4 and UCS-2 hiPSCs were frozen in mFreSR medium (Stem Cell Technologies, Vancouver, Canada) and stored under cryogenic conditions in a vapor phase liquid nitrogen dewar ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides are then stained for 1-2 days in Carmine Alum (Stem Cell Technologies; Catalog No. 07070) at RT ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 2 min in NH4Cl red blood cell lysis solution (StemCell Technologies, cat. # 07800, Vancouver, Canada), and seeded into culture plates in αMEM supplemented with 15% FBS and antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... and further expanded in Neurocult NSA with proliferation supplement (StemCell Technologies; 10ng/ml rhFGF-2, 20ng/ml rhEGF) and incubated at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... with a full medium change at day 2 and passage at day 3 using Accutase (STEMCELL Technologies, 07920).
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bioRxiv - Neuroscience 2021Quote: ... PBMCs separation was performed within 2 h of blood withdrawal using SepMate™ tubes (StemCell Technologies, BC, Canada) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were minced into ∼1–2 mm pieces before digesting with 1 × Collagenase/Hyaluronidase (STEMCELL Technologies, Catalog #: 07912) and 100 μg/mL DNase I (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by 2X dilution in Dulbecco’s Phosphate Buffered Saline with 2% Fetal Bovine Serum (Stemcell Technologies, Cat.#07905), and finally ...
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bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... the media was replaced with BrainPhys™ medium with 2% (v/v) SM1 and antibiotic-antimycotic (StemCell Technologies #5792). From 14 DIV ...
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bioRxiv - Neuroscience 2022Quote: ... neural induction was initiated using a chemically defined neural base medium (NbM) supplemented with 2 µM Dorsomorphin (StemCell Technologies), 3 µM CHIR990021 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the maintenance well using 1-2 ml cold DMEM/F12 (Stem Cell Technologies) + 15 mM HEPES + 1x GlutaMax (1:100 35050-038 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Neuroscience 2020Quote: ... Transduced hiPSCs were selected and expanded in the presence of 2 μM puromycin and cryo-stored in mFreSR (STEMCELL Technologies) or further differentiated to OPCs as described below.
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were cryopreserved at 2×107 T cells per vial in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... For comparing to hCO cultured in STEMdiff™ Neural Organoid Basal Medium 2 (hCO + STEMdiff™ NOBM, STEMCELL Technologies, 08620), the remaining organoids in each well were imaged at day 12 on a REVOLVE microscope (ECHO ...
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bioRxiv - Developmental Biology 2019Quote: Placenta cell suspensions were suspended in Iscove’s MDM with 2% fetal bovine serum and cultured using a MethoCult GF M3434 Optimum kit (STEMCELL Technologies). 10,000 cells from each sample were plated in 35-mm culture dishes (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... the iPSC were maintained in StemFlex medium and passaged 1-2 a week using ReLSR (STEMCELL Technologies, Vancouver, BC, Canada) dissociation buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... half of the medium was refreshed weekly with BrainPhys medium (BP) supplemented with 2% NeuroCult SM1 neuronal supplement (STEMCELL Technologies) and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2023Quote: B cells subsets were obtained from WT or IL17 −/− naïve mice or from mice 14 d after alloantigen immunization (2 × 10 7 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... microcapsules carrying HUES-8 cells were immersed in E8 medium for 1 h immediately after encapsulation and then transferred into E6 medium (without FGF-2 and TGF-β1, STEMCELL Technologies) for subsequent cultivation (2000 microcapsules per well) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 tumors were extracted and minced with sterile razor blades and incubated for 2 h in the presence of Collagenase (1,000 units per sample) in sterile Epicult media (Stem Cell Technology). Cells were washed with sterile filtered PBS supplemented with 1% bovine serum albumin (PBS-BSA 1% ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs were plated onto Matrigel at a density of 2×105 cells/cm2 in E8 medium containing 10 µM ROCK inhibitor (STEMCELL Technologies) and cultured overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...