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Citations for Stemcell Technologies :
101 - 150 of 780 citations for 6 Piperidino 1 purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and WTC-11 lines and cultured in 6-well plates were dissociated with TRPYLE (Stem Cell Technology), centrifuged at 300 g ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes from wild-type or HLA-A2+ BL/6 mice were depleted of Thy1.2+ cells (STEMCELL Technologies), irradiated (20 Gy ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.1 mL was plated per well in a SmartDish 6-well plate (27371, STEMCELL Technologies, USA) in duplicates ...
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bioRxiv - Bioengineering 2023Quote: ... monocytes were seeded into a 6-well plate in ImmunoCult™-ACF Dendritic Cell Medium (Stemcell Technologies), supplemented with recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were maintained on ESC-qualified Matrigel-coated 6-well plates in mTeSR plus medium (STEMCELL Technologies) with 10 μM Y-23632 (only for the initial 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the EB were transferred to a 6-well ultra-low adherent plate with Expansion Medium (Stem Cell Technologies). After 3 days ...
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bioRxiv - Immunology 2022Quote: ... 1.1ml of the mixture was slowly distributed to a well in 6-well SmartDishTM plates (StemCell Technologies, 27370). 6-well plates were incubated in a 37°C ...
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bioRxiv - Genomics 2021Quote: ... progenitor BM sorted cells were seeded (250 or 500 cells/well) into 6 well SmartDish™ (STEMCELL Technologies). After incubation for 14 days ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumors (6–8 mm in diameter) were mechanically dissociated and digested with collagenase/hyaluronidase (#07912, STEMCELL Technologies) containing DNase I (4 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... sorted cell populations were seeded (100 or 250 cells/well) into 6 well SmartDish™ (Stem Cell Technologies). After incubation for 14 days ...
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bioRxiv - Immunology 2023Quote: ... or PEP-619WW C57BL/6 mice using a negative isolation kit (Stem Cell Technologies, Vancouver, British Columbia, Canada). A low number of isolated CD4 T cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
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bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated into single cells and clustered in AggreWell 400 6-well plates (Cat. No. 34425, STEMCELL Technologies) with DMEM + 1% (v/v ...
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bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
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bioRxiv - Cell Biology 2023Quote: ... The hiPSCs were maintained on Corning Matrigel-coated 6-well tissue culture plates with mTeSR-Plus medium (STEMCELL Technologies). Cells were passaged every 4 days using ReLeSR media (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and plated into a poly-hema treated 6-cm Petri dish in media containing mTeSR1 (StemCell Technologies, Cambridge, MA) and 10μM rock inhibitor (RI ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were seeded on pre-treated (0.167 mg/mL Matrigel) 6-well plates and cultured in TeSR-E8 iPSC culture medium (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were plated on 6-well plates coated with geltrex (Life Technology) and cultured in TeSR-E8 medium (Stemcell Technology). For hiPSC-CMs differentiation ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 or 400 double-positive cells (GFP+ / mCherry+) were plated into a matrigel or laminin-coated 6 cm petri dish in mTeSR supplemented with 1X CloneR (Stemcell technologies). Individual colonies were manually picked into 96 wells approximately 10 days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...