Labshake search
Citations for Stemcell Technologies :
201 - 250 of 879 citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of Lymphoprep™ with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... Cells were then permeabilized with 0.3% Triton-X for 15 min followed by 30 min block in 0.1% Triton-X + 5% normal rat serum (Stem Cell Technologies). Cells were incubated in Drp1 p616 Ab overnight at 4°C in 0.1% Triton-X + 1% BSA and then in secondary antibodies (AF488 Goat anti-Rabbit) ...
-
bioRxiv - Microbiology 2022Quote: ... HNAE cells were collected from healthy donors (human ethics approval GLY/01/15/HREC) and expanded using Pneumacult Ex+ (Stemcell technologies). HNAE cells were differentiated at air-liquid interface in 6.5 mm transwell with a 0.4μm polyester 156 membrane (Corning ...
-
bioRxiv - Developmental Biology 2023Quote: ... NMP-MSCs were suspended as a sphere in a 15-ml conical tube and cultured in MesenCult-ACF Chondrogenic Differentiation medium (STEMCELL Technologies) for 4 weeks.
-
bioRxiv - Immunology 2023Quote: Different blood compartments (plasma, PBMCs, and RBCs) were separated by density gradient centrifugation using SepMate-15 tubes (STEMCELL Technologies, catalog #85415) to reduce blood processing time and user variability ...
-
bioRxiv - Immunology 2024Quote: Female CatchupIVM-red and FVB/N mice aged 8-15 weeks were treated with 5 µg mouse recombinant G-CSF (STEMCELL Technologies) by intraperitoneal injection (i.p. ...
-
bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
-
bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were dissociated into single cells and clustered in AggreWell 400 6-well plates (Cat. No. 34425, STEMCELL Technologies) with DMEM + 1% (v/v ...
-
bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
-
bioRxiv - Cell Biology 2023Quote: ... The hiPSCs were maintained on Corning Matrigel-coated 6-well tissue culture plates with mTeSR-Plus medium (STEMCELL Technologies). Cells were passaged every 4 days using ReLeSR media (StemCell Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... and plated into a poly-hema treated 6-cm Petri dish in media containing mTeSR1 (StemCell Technologies, Cambridge, MA) and 10μM rock inhibitor (RI ...
-
bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
-
bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
-
bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
-
bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
-
bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
-
bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
-
bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... iPSC lines were seeded on pre-treated (0.167 mg/mL Matrigel) 6-well plates and cultured in TeSR-E8 iPSC culture medium (StemCell Technologies) according to the manufacture’s guidelines ...
-
bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
-
bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... iPSCs were plated on 6-well plates coated with geltrex (Life Technology) and cultured in TeSR-E8 medium (Stemcell Technology). For hiPSC-CMs differentiation ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
-
bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
-
bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
-
bioRxiv - Bioengineering 2020Quote: ... After 72 hours the medium was replaced with 7 mL of neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 nM and smoothened agonist (SAG ...
-
bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
-
bioRxiv - Molecular Biology 2023Quote: ... NPCs were seeded in four Matrigel-coated 15-cm dishes per condition and allowed to reach 70-80% confluency in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies). Then ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
-
bioRxiv - Immunology 2023Quote: ... PBMC were isolated from blood collected in ethylenediaminetetraacetic acid-anticoagulated (EDTA) tubes by lymphocyte separation medium density gradients (Stemcell technologies, cat# 07801) and resuspended in PRMI 1640 medium supplemented with 10% FCS ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
-
Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... 200 or 400 double-positive cells (GFP+ / mCherry+) were plated into a matrigel or laminin-coated 6 cm petri dish in mTeSR supplemented with 1X CloneR (Stemcell technologies). Individual colonies were manually picked into 96 wells approximately 10 days after transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
-
bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
-
bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
-
bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
-
bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
-
bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
-
bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
-
bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...