Labshake search
Citations for Stemcell Technologies :
251 - 300 of 473 citations for 6 METHYLAMINO 5 NITROQUINOXALINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
-
bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
-
bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated into single cells and seeded onto matrigel coated plates at a density of 400-600k cells per well of a 6 well plate in mTeSR (Stem Cell Technologies) containing 10 µM Rock inhibitor (Y27632 ...
-
bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
-
bioRxiv - Physiology 2020Quote: ... Undifferentiated hiPSCs were maintained on Vitronectin XF (5 μg/mL, Stem Cell Technologies) coated tissue culture dishes as per manufacturer recommendation with either mTeSR medium with supplementation or mTeSR PLUS medium with supplementation (Stem Cell Technologies) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 300 U/mL of hyaluronidase and 5 mg/mL DNAse I (StemCell Technologies) under gentile agitation for 10 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... 300 U/mL of hyaluronidase and 5 mg/mL DNAse I (StemCell Technologies) under gentile agitation for 10 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... and passaged every 4-5 days using Gentle Cell Dissociation Reagent (StemCell Technologies). For organoid seeding ...
-
bioRxiv - Cell Biology 2019Quote: ... a 5-minute treatment with 1 U/ml dispase (Stem Cell Technologies 07913) and 0.1 mg/ml DNase (Stem Cell Technologies 07900) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Every 4-5 days cells were passaged using ReLeSR reagent (Stemcell tech, 05872).
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS and 1:10 dilution of collagenase/hyaluronidase (STEMCELL Technologies) at 37°C for 3 hr ...
-
bioRxiv - Cancer Biology 2023Quote: ... pelleted cells were resuspended with pre-warmed 5 mg/ml dispase (STEMCELL Technologies) supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with a final concentration of 5 μM XAV-939 (STEMCELL Technologies, #72672) on 1:100 geltrex (GIBCO ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were split every 5-7 days with accutase (Stem Cell Technologies, #07920) (Bajpai et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
-
bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
-
bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
-
bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
-
bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
-
bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
-
bioRxiv - Developmental Biology 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
-
bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were dissociated with 5 ml Accutase™ cell dissociation reagent (STEMCELL technology, 07920). After washing once with PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... unfractionated nucleated BM cells (5×105) were suspended in methylcellulose medium (M3231, StemCell Technologies) with 0.1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... unfractionated nucleated BM cells (5×105) were suspended in methylcellulose medium (M3231, StemCell Technologies) with 0.01 ng/ml murine GM-CSF (Peprotech ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were passaged every 5-7 days using ReLeSR (STEMCELL Technologies, Vancouver, BC, Canada) and maintained within passages 20 to 50.
-
bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
-
bioRxiv - Neuroscience 2019Quote: ... Cultures were maintained at 37°C at 5% CO2 in BrainPhys media (StemCell Technologies) without phenol red supplemented with SM1 (StemCell Technologies ...
-
bioRxiv - Bioengineering 2021Quote: ... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
-
bioRxiv - Cell Biology 2019Quote: ... Routine passaging every 4-5 days was performed using ReLeSR (Cat. # 05873; STEMCELL Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... at a density of 5×103 cells/ml in Complete MammoCult Medium (StemCell Technologies). After seven days ...
-
bioRxiv - Cell Biology 2023Quote: ... Routine passaging was performed every 4-5 days using ReLeSR (Cat. # 05873; STEMCELL Technologies) according to manufacturer’s instructions in E8/VTN or by treatment with Collagenase IV (Cat ...
-
bioRxiv - Developmental Biology 2024Quote: ... iPSCs lines were passaged every 5 days using Gentle Cell Dissociation Reagent (Stemcell Technologies) and plated in mTesr1 in the presence of 10 uM Y-27632 (Stemcell Technologies) ...
-
bioRxiv - Immunology 2023Quote: T cells were purified from the spleen and peripheral lymph nodes of C57BL/6 or OT-I mice by negative selection (StemCell Technologies, Vancouver, BC, Canada). Cells were cultured in T cell medium (TCM ...
-
bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
-
bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
-
bioRxiv - Developmental Biology 2022Quote: ... dissociated into single cells (accutase, Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and fixed (Foxp3/Transcription Factor Staining Buffer Set ...
-
bioRxiv - Pathology 2019Quote: ... iPSCs were exposed to N2 media containing 5 mL N2 Supplement-B (Stemcell Technologies 07156), 0.5mL 55mM β-mercaptoethanol (Life technologies 21985-023) ...
-
bioRxiv - Genomics 2022Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
-
bioRxiv - Genomics 2022Quote: ... the media was replaced with mTeSR1 supplemented with 5 uM of CHIR99021 (STEMCELL Tech., 72052) for 24 hours ...
-
bioRxiv - Genetics 2022Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (Stemcell Technologies).
-
bioRxiv - Cancer Biology 2020Quote: ... and then incubated in a digestion buffer of 5 Units/mL dispase (Stemcell Technologies, #07913) and 0.1 mg/mL DNase I (Worthington ...
-
bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...