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Citations for Stemcell Technologies :
451 - 500 of 667 citations for 6 Isobutoxy 5 methylpyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
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bioRxiv - Developmental Biology 2022Quote: ... dissociated into single cells (accutase, Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and fixed (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Pathology 2019Quote: ... iPSCs were exposed to N2 media containing 5 mL N2 Supplement-B (Stemcell Technologies 07156), 0.5mL 55mM β-mercaptoethanol (Life technologies 21985-023) ...
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bioRxiv - Genomics 2022Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
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bioRxiv - Genomics 2022Quote: ... the media was replaced with mTeSR1 supplemented with 5 uM of CHIR99021 (STEMCELL Tech., 72052) for 24 hours ...
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bioRxiv - Genetics 2022Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (Stemcell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... and then incubated in a digestion buffer of 5 Units/mL dispase (Stemcell Technologies, #07913) and 0.1 mg/mL DNase I (Worthington ...
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bioRxiv - Genomics 2021Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours.
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded (between 5×103 and 1×104) in methylcellulose (Methocult H4100, StemCell Technologies) in triplicate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 4 to 5 days using ReLeSR (StemCell Technologies, catalog no. 05872). We performed characterization of our iPSCs by immunofluorescence ...
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bioRxiv - Cancer Biology 2023Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37 °C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
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bioRxiv - Immunology 2023Quote: ... was pelleted and resuspended in 5 mL of nycoprep (Axis Shield) or OptiPrep (STEMCELL Technologies), prepared according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 μM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Neuroscience 2023Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 µM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 in StemSpan (Stem Cell Technology #09650) supplemented with TPO (0,125ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... mTeSR1 was supplemented with the ROCK inhibitor Y-27632 (STEMCELL Technologies, 72308, 5 μM final).
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of ±8.75 × 104 cells/cm2 or 3 × 105 cells/ml in mTeSR medium (Stem Cell Technologies, 85850) supplemented with 1:100 RevitaCell Supplement (Gibco ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2020Quote: ... WBM (5×104 cells) were plated in duplicate wells of Methocult GF M3434 methylcellulose (StemCell Technologies) according to the manufacturer’s suggestions ...
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bioRxiv - Cell Biology 2020Quote: ... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
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bioRxiv - Immunology 2019Quote: ... Human Cord Blood (CB) purified CD34+ cells from 5 different donors were obtained from STEMCELL Technologies and resuspended in StemSpan Expansion Media – SFEM II (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% CO2 and ambient oxygen level on Geltrex coated plates in mTeSR1 medium (STEMCELL Technologies, 05850) with daily medium change ...
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bioRxiv - Developmental Biology 2021Quote: ... coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2) in mTeSR1 (StemCell Technologies). These were passaged every 4-6 days with Accutase (#A6964 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stemgent or Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the medium was replaced with RPMI 1640 containing 5 μM XAV-939 (Stem Cell Technologies, Canada), 500 μg.mL-1 BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were centrifuged for 5 min at 900xg then resuspended in BrainPhys media (05790, STEMCell Technologies) with 1% penicillin-streptomycin (15070063 ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Cell Biology 2019Quote: ... All adherent cells in each well were detached by trypsinization and plated in 3 mL MethocultTM H4034 (Stemcell Technologies, Vancouver, Canada). After 14 to 16 days of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Bioengineering 2022Quote: ... printed constructs were incubated for 20 min in 5 mg mL-1 collagenase type I (STEMCELL Technologies) in PBS supplemented with 5% FBS and then lysed with RLT buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and maintained in culture medium containing mTeSR plus basal medium and 5 x supplement (Stem Cell Technologies). Thiazovivin (2 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). In the morning ...
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bioRxiv - Neuroscience 2021Quote: ... Cultures were fed every other day and passaged every 5–7 days by ReLeSR™ (STEMCELL Technologies).
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bioRxiv - Pathology 2019Quote: ... pelleted at 800 rpm for 5 min and resuspended in mTeSR1 plus 5μM Y27632 dihydrochloride (Stemcell Technologies) for the first 24 hours to facilitate cell survival ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by cardiac specification using RPMI B27-containing 5 μM IWP-4 (Stem Cell Technologies) for another 3 days ...
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bioRxiv - Cancer Biology 2022Quote: ... transduced AML cells were cultured on MS-5 cells (DSMZ) in Myelocult media H5100 (StemCell Technologies Inc.) with rhIL-6 ...
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bioRxiv - Bioengineering 2023Quote: ... The iPSCs were routinely passaged every 5-7 days using ReLeSR™ Passaging Reagent (Stemcell Technologies, Canada). The iPSCs were then differentiated into SCPCs based on a modified protocol to generate spinal motor neurons (Hor et al. ...