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Citations for Stemcell Technologies :
301 - 350 of 591 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cells were passaged using a 5 min incubation with Accutase (StemCell Technologies) on Matrigel-coated plates in mTeSR1 media supplemented with 10 μM of Y-27632 (Cayman Chemicals) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were further dissociated in Dispase 5 U/mL (STEMCELL Technologies, 07913) plus 50 µg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was supplemented with 5 or 10mM CHIR99021 (Stemcell technologies 72052), or with 200 ...
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bioRxiv - Microbiology 2024Quote: ... medium was replaced by ODM supplemented with 5 µM CHIR99021 (StemCell technologies) and 10 µM Y-27632 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were exposed to the Wnt signaling inhibitor IWP-4 (STEMCELL Technologies, cat. 72552) for 2 days from days (D ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were fed daily and split every 4-7 days using ReLeSR (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Genetics 2021Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... all the cells transfected with Sendai virus were transferred into a 6-well plate (30,000 cells per well) each well containing ~150,000 mouse embryonic fibroblast (MEF) feeder cells (Stemcell Technologies Inc. Vancouver, Canada). The MEF plate preparation was conducted the day before plating the reprogramming cells as explained elsewhere (28) ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 07180)-coated tissue culture plates and split in a ratio of 1:6 to 1:12 every four to six days with Gentle Cell Dissociation Reagent (STEMCELL Technologies, 07174). Alveolar differentiation was induced as previously described (Riva et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated into single cells and seeded onto matrigel coated plates at a density of 400-600k cells per well of a 6 well plate in mTeSR (Stem Cell Technologies) containing 10 µM Rock inhibitor (Y27632 ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Physiology 2020Quote: ... Undifferentiated hiPSCs were maintained on Vitronectin XF (5 μg/mL, Stem Cell Technologies) coated tissue culture dishes as per manufacturer recommendation with either mTeSR medium with supplementation or mTeSR PLUS medium with supplementation (Stem Cell Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 300 U/mL of hyaluronidase and 5 mg/mL DNAse I (StemCell Technologies) under gentile agitation for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 300 U/mL of hyaluronidase and 5 mg/mL DNAse I (StemCell Technologies) under gentile agitation for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... a 5-minute treatment with 1 U/ml dispase (Stem Cell Technologies 07913) and 0.1 mg/ml DNase (Stem Cell Technologies 07900) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS and 1:10 dilution of collagenase/hyaluronidase (STEMCELL Technologies) at 37°C for 3 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... pelleted cells were resuspended with pre-warmed 5 mg/ml dispase (STEMCELL Technologies) supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with a final concentration of 5 μM XAV-939 (STEMCELL Technologies, #72672) on 1:100 geltrex (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Developmental Biology 2024Quote: ... cells were split every 5-7 days with accutase (Stem Cell Technologies, #07920) (Bajpai et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were passaged every 4 to 7 days with ReLeSR reagent (StemCell Technologies catalog #05872) to ensure that only undifferentiated stem cells remained in the cultures ...
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bioRxiv - Genomics 2021Quote: ... and 4) one cord blood (CB001) collected with informed consent by StemCell Technologies (catalog #70007) (Table S1) ...
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bioRxiv - Cell Biology 2019Quote: ... medium is supplemented with 10 ng/ml activinA (STEMCELL technologies, 78001; removed at day 4); on day 2 ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...