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Citations for Stemcell Technologies :
401 - 450 of 486 citations for 6 Chloro 4 iodopyridin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Cell Biology 2019Quote: ... All adherent cells in each well were detached by trypsinization and plated in 3 mL MethocultTM H4034 (Stemcell Technologies, Vancouver, Canada). After 14 to 16 days of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Neuroscience 2020Quote: The human embryonic stem cell (hESC) line harbouring the GPF reporter at the 3’UTR of CRX locus was expanded in mTeSR™1 (StemCell Technologies) at 37°C and 5% CO2 on 6 well plates pre-coated with Low Growth Factor Matrigel (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... for 4 days to induce iMSCs before the medium was changed to Complete MesenCult™-ACF Plus Medium (STEMCELL Technologies) for an additional 2 days and then the cells were transferred into a new 24-well plate without an MEF feeder layer ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Microbiology 2021Quote: ... A nasal brushing of the turbinate was acquired using 3-mm bronchial cytology brush and placing the biopsy into warm PneumaCult-Ex Plus Medium (STEMCELL Technologies, Cambridge, UK). The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The microglia cell differentiation through 3 steps: 1) Embryoid Body (EB) formation: using Spin-EBs formation method with AggreWellsTM800 (Stemcell Technologies, Catalog # 34825). briefly ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...