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Citations for Stemcell Technologies :
301 - 350 of 611 citations for 6 Chloro 2 2 dimethyl 4 chromanone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Immunology 2022Quote: The collected blood was 1:1 diluted with 2% FBS and was added to 7ml of Lymphoprep™ density gradient medium in SepMate-15 tubes (StemCell Technologies). The red blood cells ...
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bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: The iPSCs were obtained from the National Institutes of Health Cell Repositories and maintained on Matrigel (BD)-coated 6-well plates in mTeSR Plus medium (STEMCELL Technologies) using a feeder-free culture protocol ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plates using mTeSR1 medium (Stem Cell Technologies, 83850). Neuronal differentiation was chemically induced by culturing hiPSC colonies in suspension in ultra-low attachment T75 flasks (Corning ...
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bioRxiv - Microbiology 2020Quote: ... coated 6-well plates in mTeSR1 media (Stem Cell Technologies) at 37°C in 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged 1:6 using RELESR (StemCell Technologies 05872) for detachment of hESCs ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10-6 M hydrocortisone (74142, Stem Cell Technologies) and 1% penicillin streptomycin ...
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bioRxiv - Developmental Biology 2023Quote: ... coated 6 well plates in mTeSR1 medium (StemCell Tech, 85850) at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coated 6-well plate in mTeSR1 (STEMCELL Technologies, cat # 85870) media and were passaged every 4 days at a ratio of 1:6 t o1:8 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... coated 6-well plates in mTESR1 medium (Stem Cell Technologies). Details on the cell lines used are available in Supplementary figures ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples were extracted from differentiated NPCs or undifferentiated NPCs treated for 2 hrs with STEMdiff™ Forebrain Neuron Differentiation medium (Stem Cell Technologies) for differentiated NPCs ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: AGMs of E10.5 embryos were dissected in PBS with 7% fetal calf serum (FCS) and penicillin/streptomycin (100 U/mL) and cultured as explants for 2 days in medium consisting of Stemspan (Stem Cell Technologies, cat # 09600), 20% fetal calf serum ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
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bioRxiv - Neuroscience 2021Quote: ... coated 6-well plates and maintained in mTeSR1 medium (STEMCELL Technologies). Nociceptor differentiation was performed as previously published (18 ...
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bioRxiv - Neuroscience 2022Quote: ... 6-well plate for 14 days using Brainphys (Stem Cell Technologies) 1X B27 Supplement (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... and passaged once every 5-6 days using Dispase (StemCell Technology). To maintain pluripotency and limit spontaneous differentiation ...
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bioRxiv - Bioengineering 2021Quote: ... All human iPSCs were cultured on Matrigel (Corning)-coated 6-well plates with mTeSR™1 medium (StemCell Technologies). Full-volume medium replacement with mTESR1 was performed daily ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6-well plates and maintained in mTeSR1 medium (STEMCELL Technologies). Nociceptor differentiation was performed as previously published.
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bioRxiv - Cancer Biology 2023Quote: ... Colony counting was performed manually using STEMgrid-6 (StemCell technologies, #27000). Images of the well plates were taken using Keyence BZX-800 inverted fluorescence microscope ...
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bioRxiv - Developmental Biology 2023Quote: ... coated 6-well dishes in mTeSR Plus (StemCell Technologies, #100-0276) supplemented with penicillin/streptomycin (P/S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-integrin-⍺-6 (Stem Cell Technologies; Vancouver, BC, Canada; cat:60037), anti-β-human-chorionic-gonadotrophin (β-hCG ...
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bioRxiv - Bioengineering 2024Quote: ... and 6 × 103 human bone marrow CD34+ cells (STEMCELL Technologies, 70002.3) into a collagen-based hydrogel according to the protocol described previously (49 ...
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bioRxiv - Developmental Biology 2022Quote: ... replated at a density of 1.58 × 105 cells/cm2 in Vitronectin XF-coated 24 well plate into cardiomyocyte media (RPMI, 2% B27 supplement plus insulin [Life Technologies Australia, Cat.#17504001], 10% FBS, and 10 µM Y-27362 ROCK inhibitor [Stem Cell Technologies, Cat.#72308]) and cultured overnight ...
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... anti-SSEA-4 antibody (STEMCELL Technologies), anti-Sox17 antibody (R&D System) ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-well plates were pretreated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min incubation at room temperature followed by two rinses with equal volumes of fresh HBSS ...
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bioRxiv - Immunology 2021Quote: ... coated 6-well plates in mTeSR Plus (STEMCELL Technologies, Vancouver, BC, Canada) with medium changes every other day ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-6 and G-CSF (all from Peprotech or Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... and seeded to microwells (6-well AggreWell 400 plates, Stem Cell Technologies) at a density of 800-1000 cells per microwell using the manufacturer’s recommended protocol ...