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Citations for Stemcell Technologies :
451 - 500 of 1179 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Immunology 2023Quote: CD63+ DCs or CD63- control DCs isolated from the LNs of Balb/c mice were sorted by FACS as described previously and cultured with naïve CD4+ T cells magnetically isolated from Fox3-GFP+ mice (C57Bl/6 background) using EasySep Mouse Naïve Cd4+ T cell isolation kit (Stemcell Technologies, Cat# 19765) at a ratio of 1:5 DC:T cell for 5 days in RPMI containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated into single cells and seeded onto matrigel coated plates at a density of 400-600k cells per well of a 6 well plate in mTeSR (Stem Cell Technologies) containing 10 µM Rock inhibitor (Y27632 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected with 2 mL mTeSRplus (Stem Cell Technologies, 05825), span down and resuspended in 1 mL mTeSRplus ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Bioengineering 2021Quote: ... additionally supplemented with cytokines (Human Recombinant IL-2; Stemcell Technologies - #78036). To stimulate the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... PHA was washed and IL-2 (50 U/ml, StemCell Technology) was added to the medium and replaced every 2 days for a time period of 5 days.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... 2% BSA (Miltenyi) and 10 uM ROCK inhibitor (StemCell technologies 72302). For all wash steps ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 3 × 106 single cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8 flex medium supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred at 3 x 106 cells to a well of an AgrreWell800 plate (Stem Cell Technologies) in STEMdiff Neural Induction Medium + SMADi (Stem Cell Technologies ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: ... approximately 3×104 total Ai14 BM cells were plated per well in MethoCult (M3434, Stem Cell Technologies) enriched with rmSCF ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
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bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Molecular Biology 2021Quote: ... 2 million cells were passaged per each well using Accutase (StemCell Technologies) into the STEMdiff SMADi neural induction medium (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). L0125 and L0512 GBM tumorspheres were kindly provided by Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). S24 Tdtomato/GFP cells were kindly gifted by Dr ...
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bioRxiv - Neuroscience 2022Quote: ... Heparin solution 2 μg/ml (cat# 07980, Stem Cell Technologies, Vancouver, Canada), EGF 20 ng/ml (Cat#315-09 ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were grown in StemSpan SFEM (STEMCELL Technologies, Vancouver, Canada) supplemented with 50ng/mL stem cell factor ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Cancer Biology 2023Quote: ... and fed IL-2 (50 U/mL; Stem Cell Technologies, Vancouver, Canada) every 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HUDEP 2 cells were cultured in Stemspan Media (SFEM II - STEMCELL Technologies) with 50ng/ml SCF (ImmunoTools) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µM (for iPSC) or 5 µM (for ESC) XAV939 (Stemcell Technologies), and 10 µM Y27632 ...
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bioRxiv - Bioengineering 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies, Cat# 78036.3). To prepare 3 mg/ml collagen-I gel ...
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bioRxiv - Immunology 2023Quote: T cells were purified from the spleen and peripheral lymph nodes of C57BL/6 or OT-I mice by negative selection (StemCell Technologies, Vancouver, BC, Canada). Cells were cultured in T cell medium (TCM ...