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Citations for Stemcell Technologies :
401 - 450 of 541 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and resuspended to a final concentration of 10 000 cells/50 μL in EB media: mTeSR™ Plus medium (StemCell Technologies), 50 ng/mL BMP-4 (R&D ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at −80°C until analysis ...
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bioRxiv - Biochemistry 2019Quote: ... collected by 5 min centrifugation at 300 × g and resuspended in mTeSR1 supplemented with 10 µM Rock inhibitor Y-27632(2HCl) (72304, StemCell Technologies) for the first day.
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bioRxiv - Physiology 2020Quote: ... Single-cell suspensions were plated at a density of 1.2 × 105 cells/cm2 and cultured overnight in mTeSR medium supplemented with 10 μM Y-27632 dihydrochloride (Stem Cell Technologies). Once the monolayer reached approximately 80% confluence (usually the following day) ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Neuroscience 2021Quote: ... The media was changed daily and the iPSC cultures were split into 1:6-1:10 every 5 days using ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2021Quote: ... The resulting single cell suspension was than washed with 10 mL of cold RPMI and resuspended in 1 mL of Robosep buffer (STEMCELL technologies) prior to the negative selection of B- or T-cells ...
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bioRxiv - Developmental Biology 2022Quote: ... single hESCs were seeded into a spinner flask at a density of 1e6 cells/mL in mTeSR1 media containing 10 μM Rock inhibitor Y-27632 (STEMCELL Technologies) to allow formation of clusters ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs were plated onto Matrigel at a density of 2×105 cells/cm2 in E8 medium containing 10 µM ROCK inhibitor (STEMCELL Technologies) and cultured overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Developmental Biology 2022Quote: ... Matrigel-coated wells of a 24-well plate with UPC medium supplemented with 10 µM Y-27632 ROCK inhibitor (STEMCELL Technologies) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfected cells were resuspended in pre-warmed Essential 8™ medium supplemented with 10 µM ROCK-inhibitor (Y-27632) (Stemcell Technologies) and seeded into two wells of a Matrigel® coated 6-well plate ...
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bioRxiv - Developmental Biology 2020Quote: ... Undifferentiated hESCs were aggregated by preparing a single cell suspension in mTeSR1 media (STEMCELL Technologies; supplemented with 10 µM Y-27632) at 1 × 106 cells/mL and overnight culture in six-well ultra-low attachment plates (Costar ...
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bioRxiv - Developmental Biology 2021Quote: ... Frozen vials of iPSC were thawed into mTeSR plus media supplemented with 10 µM ROCK inhibitor (Y27632, STEMCELL Technologies, catalog #72304) and the media changed after 2 days.
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Developmental Biology 2022Quote: ... For HLC differentiation cells were seeded onto Matrigel™ coated 24-well tissue culture plates at 130,000 cells/ cm2 in mTeSR™ Plus containing 10 μM ROCK inhibitor (STEMCELL Technologies). After 24 hours the differentiation protocol was commenced using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... before centrifugation at 300 xg for 10 minutes followed by resuspension in 100 μl of CryoStor-CS10 freezing media (StemCell Technologies). The UVC preparations for use in non-human primate studies were analyzed for endotoxin levels (Wickham Laboratories Ltd ...
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bioRxiv - Developmental Biology 2021Quote: ... approximately half of the neuruloids on each coverslip were scraped off and treated for 10 min at 37 °C with Accutase (Stemcell Technologies). The remaining neuruloids were fixed for immunofluorescence analysis as a quality control ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Bioengineering 2020Quote: ... MSPCs were isolated from the crushed bone following a commercially available protocol and cultured for 10 days prior to collection and use (#05513, Stemcell Technologies).
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bioRxiv - Cancer Biology 2021Quote: Tumor tissue excised from the brain was mechanically dissociated using a 70 um cell strainer in phosphate-buffered saline (PBS) with 10% FBS and 0.1 mg/mL DNAse I (Stem Cell Technologies). Cells were then resuspended in 25% isotonic Percoll solution and centrifuged for 20 minutes at 18°C with acceleration and deceleration set to minimum ...
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bioRxiv - Immunology 2021Quote: ... PBMC and plasma samples were isolated after whole blood centrifugation 1200 g for 10 minutes at room temperature in SepMate tubes (STEMCELL Technologies). Plasma was stored at -800C until analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The detached colonies were then pelleted at 300g(av) and reseeded with mTeSR Plus medium supplemented with 10 µM Rock Inhibitor (Y-27632, STEMCELL Technologies). At passage 10 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Neuroscience 2023Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
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bioRxiv - Cancer Biology 2023Quote: ... and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Bioengineering 2023Quote: ... we single cell sorted ATTO550 positive cells for monoclonal expansion in 96-well plates containing their corresponding dTMP-supplemented culture media (hiPSC additionally supplemented with 10% Clone R (StemCell Technologies)) ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were dissociated into single cells using Accutase and plated on 10 µg/mL Vitronectin XF (Cat. No. 07180, STEMCELL Technologies)-coated plates with 10 µM of Y27632 (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with specific neuronal marker CD24-FITC Clone M1/69 antibody(38) (Stemcell Technologies, Canada) diluted 1:100 for 30 minutes on ice ...
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...