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Citations for Stemcell Technologies :
351 - 400 of 677 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... then passage into a primary methylcellulose-based medium containing murine Stem Cell Factor (mSCF) following the manufacturer’s protocol (STEMCELL Technologies), where they differentiate into pluripotent ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... medium from the upper chamber was removed and replaced with ATDC5 medium containing 0 or 0.1 ng/ml IL-1α (STEMCELL Technologies). Following two additional days of incubation ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 7 days and cultured at a density of 2e5-1e6 cells/ml ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 4 days ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Bioengineering 2024Quote: ... The tumors were harvested and minced using a scalpel and incubated in DMEM media containing collagenase/hyaluronidase (STEMCELL Technologies, #07912) at 37 °C for 60 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The KnockOut medium was supplemented with a two-inhibitor cocktail (2i) containing 100 nM LDN-193189 (Stemcell Technologies, Vancouver, Canada) and 10 μM SB-431542 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... and the remaining cells were collected into 96-well plates (1 cell per well) containing Dulbecco’s modified Eagle’s medium F-12 (DMEM/F-12) with MesenCult™-SF attachment substrate (StemCell Technologies) and 1% antibiotic-antimycotic (Life Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... and plated on Matrigel coated plates containing mTeSR Plus supplemented with 10 µM Y-27632 (STEMCELL Technologies, cat. no. 72304). Cells were maintained at 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... antibodies using EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies). Naïve CD4 T cells (0.1million ...
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bioRxiv - Cancer Biology 2021Quote: ... heparin (2 mg/mL 0.2% Heparin Sodium Salt in PBS; STEMCELL technologies, Cat#07980), antibiotic-antimycotic (1X ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were fed with 2 ml of HemA medium (HPC differentiation kit, StemCell Technologies) on day 0 and half-fed with 1 ml on day 2 ...
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bioRxiv - Neuroscience 2021Quote: ... culture medium was replaced with 2 mL/well iHSC Medium A (StemCell Technologies #05310) on differentiation Day 0 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
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bioRxiv - Immunology 2020Quote: ... and 2.5μl/ml anti-CD3/23/2 ImmunoCult T cell activator (StemCell Technologies #10970). Medium was changed on day 3 and samples were assessed by flow cytometry and ELISA on day 8.
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bioRxiv - Genetics 2020Quote: ... HUDEP-2 cells were cultured in a base medium of SFEM (Stemcell Technologies 9650) containing to a final concentration of dexamethasone 1uM (Sigma D4902-100MG) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Immunology 2020Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
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bioRxiv - Bioengineering 2023Quote: ... Gentle cell dissociation reagent and APEL(2) Medium were purchased from Stem Cell Technologies. Primary antibodies used were mouse anti-Oct 3/4 (Santa Cruz Biotechnology – SC-5279) ...
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bioRxiv - Immunology 2022Quote: ... + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies, Canada). The culture media was replenished every three days with fresh media containing 10 IU of IL-2 and 10 ng/ml IL-15 ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were then treated with 2 ml pre-warmed Accutase (AT104, Stem Cell Technologies) and the vessel was incubated at 37ºC for 10 mins ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 hiPSCs were cultured in mTeSR™1 medium (Stem Cell Technologies) on Matrigel (Corning)-coated plates following the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... and colonies gently lifted by 2 min incubation in ReLeSR (Stem Cell Technologies, #05872) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: hPSCs were cultured for at least 2 passages after thawing in mTeSR1TM (STEMCELL Technologies) before differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... and routinely split 1-2 times a week with ReLesSR™ (Stem Cell Technologies) without ROCKi as described previously 43 ...
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bioRxiv - Bioengineering 2024Quote: ... HUDEP-2 cells were maintained in an expansion medium: StemSpan SFEM (STEMCELL Technologies, 09650) with 50 ng ml−1 human stem cell factor (PeproTech ...
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bioRxiv - Systems Biology 2024Quote: ... APCs were treated with the pro-inflammatory cytokines interleukin 6 protein (IL-6, Stem Cell technologies), or tumour necrosis factor alpha (TNFα ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... setting the instrument to deposit one cell into each well in a 96-well plate containing working medium with 10% CloneR cloning supplement (Stemcell Technologies). Filled plates were transferred to the 37°C incubator and left undisturbed for 48 hours after sorting ...
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... were directly sorted into a 96-well tissue culture plate containing 100 μL of differentiation media: StemSpan (Catalog #09650, StemCell Technologies), 1% Penicillin+Streptomycin ...
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bioRxiv - Bioengineering 2022Quote: The colony-forming unit (CFU) assay was performed by seeding 500 cells in six-well plates containing MethoCult Enriched (StemCell Technologies) after 4 days of editing ...
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bioRxiv - Developmental Biology 2022Quote: ... single hESCs were seeded into a spinner flask at a density of 1e6 cells/mL in mTeSR1 media containing 10 μM Rock inhibitor Y-27632 (STEMCELL Technologies) to allow formation of clusters ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Developmental Biology 2022Quote: ... For HLC differentiation cells were seeded onto Matrigel™ coated 24-well tissue culture plates at 130,000 cells/ cm2 in mTeSR™ Plus containing 10 μM ROCK inhibitor (STEMCELL Technologies). After 24 hours the differentiation protocol was commenced using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Bioengineering 2021Quote: 5 wt% GelMAL-peptide and 5 wt% GelMA hydrogels containing 105 LSK/mL were immediately placed in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
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bioRxiv - Genetics 2020Quote: ... to each well for 1h at room temperature) containing 0.5ml complete ReproTeSR medium (ReproTeSR basal medium with 25X ReproTeSR supplement) (Stem Cell Technologies). After 24h incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... they were collected and moved to a new 96-well plate containing 150 µl of induction media (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) per well ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured for 72 hours in 96-well plates (2X105-5X105 cells per well) containing StemPro (Stem Cell Technologies) media supplemented with human IL-6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...