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Citations for Stemcell Technologies :
351 - 400 of 1338 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: PBMC were purified from blood samples from GD patients using Lymphoprep™ reagent and SepMate™ tubes (Stemcell Technologies, Vancouver, Canada). Lymphoprep™ was added to the lower compartment of the SepMate tube ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from HNAE cells apical side (airlift) and provided with Pneumacult ALI basal media from HNAE cells basolateral side (Stemcell technologies). HAE cells were fully differentiated and ready to use after 28 days post-airlift ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: PBMCs from three independent donors were isolated from leukapheresis products using Lymphoprep density gradient centrifugation and SepMate PBMC isolation tubes (StemCell Technologies), under the Weatherall Institute of Molecular Medicine ...
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bioRxiv - Cell Biology 2024Quote: ... Synthetic sgRNAs were purchased from MilliporeSigma and Synthego (listed in Table S3) and ArciTect Cas9-eGFP and SpCas9-2NLS were purchased from STEMCELL Technologies and Synthego ...
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bioRxiv - Microbiology 2023Quote: ... in complete growth media from LifeLine Cell Technology (LL-0023) and subsequently differentiated to air with complete Pneumacult ALI media (05001) from Stemcell Technologies. Cells were incubated for approximately two weeks until appearance of ciliated cells and mucus production was seen (53) ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Microbiology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats from anonymized healthy blood donors by Ficoll density centrifugation using Lymphoprep (Stemcell Technologies). Buffy coats were provided by the Blood Donation Service Zurich ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Immunology 2024Quote: ... with minor modifications.18 CD34+ cells were isolated directly from PBMCs or from enriched HSPCs (RosetteSep Hematopoietic Progenitor Enrichment Cocktail Kit, Stemcell Technologies) via immunomagnetic isolation (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human peripheral blood mononuclear cells from healthy donors were thawed from cryostorage in ImmunoCult-XF medium (Stem Cell Technologies # 100-0956) containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970 ...
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bioRxiv - Immunology 2021Quote: Human NK were isolated from discarded Leukopaks from healthy volunteer blood donors using the RosetteSep™ human NK enrichment protocol (StemCell Technologies), followed by Ficoll (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Cortical organoid cultures were differentiated from the healthy iPSC cell line according to the specification of the cerebral organoid kit from StemCell Technologies (#08570) that relies on an established protocol (54) ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PBMCs of anonymous healthy donors were isolated from buffy coats purchased from the Beijing Red Cross Blood Center using density gradient cell separation by Ficoll (Lymphoprep™, STEMCELL Technologies) following the protocol approved by the Institutional Review Board of School of Medicine ...
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bioRxiv - Neuroscience 2023Quote: Mature astrocytes were created from astrocyte precursor cells using the STEMdiff Astrocyte Maturation media kit from STEMCELL Technologies (Cat No. 100-0016) following their guidelines for Matrigel-coated 6-well plates ...
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Developmental Biology 2021Quote: ... Adult BM mononuclear cells were purchased from StemCell Technologies, Canada (cat no ...
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bioRxiv - Genomics 2020Quote: Cell culture reagents were obtained from Stem Cell Technologies unless otherwise indicated ...
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bioRxiv - Neuroscience 2021Quote: ... All stem cell culture reagents were from STEMCELL technologies. We maintained and differentiated hESCs according to STEMCELL protocols.
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bioRxiv - Neuroscience 2021Quote: ... cells were switched from TeSR E8 (StemCell Technologies 05990) to mTeSR 1 (StemCell Technologies 85850) ...
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bioRxiv - Cell Biology 2020Quote: Human CB CD34+ cells were purchased from STEMCELL Technologies GmbH (Cologne ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from blood using LymphoprepTM (StemCell Technologies), and then cryopreserved in 90% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and 10 μM A83-01 (all from Stemcell Tech). Y27 was removed from medium 24 hours after initial plating ...
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bioRxiv - Cell Biology 2022Quote: ... or with Accutase (both obtained from STEMCELL Technologies, Inc.). Then ...
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bioRxiv - Molecular Biology 2024Quote: Primary T-cells were purchased from StemCell Technologies (#70024). Cells were cultured in X-Vivo 15 media supplemented with 5% human serum and 10 ng/mL of IL-2 ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purchased from StemCell Technologies. Viability was assessed via DAPI staining ...
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bioRxiv - Genetics 2024Quote: Cryopreserved PBMCs were obtained from StemCell Technologies (Catalog # 70025). PBMCs were thawed and CD3+ T cells were isolated immediately by Pan T Cell Isolation Kit (Miltenyi # 130-096-535) ...
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bioRxiv - Immunology 2024Quote: ... cord blood mononuclear cells were ordered from STEMCELL Technologies (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional BMMCs were purchased from STEMCELL Technologies (Vancouver, Canada). Lists of patient samples utilized in this study were provided in Table 1 and Table 3-6 ...
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bioRxiv - Immunology 2023Quote: ... using 50 ml SepMate tubes (all from Stemcell technologies). Briefly ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained from Stemcell Technologies (Vancouver, BC). Samples were collected from donors and anonymized prior to providing for research purposes ...
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bioRxiv - Immunology 2023Quote: Frozen human PBMCs from 5 healthy donors (Stemcell Technologies, Cat# 70025.2 ...
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bioRxiv - Molecular Biology 2024Quote: ... purchased from STEMCELL Technologies (Catalog #200-0165, Lot #2107423002), were cultured in RPMI supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... from Thermo Fisher Scientific and BrainPhysTM neuronal culture medium from STEMCELL Technologies. NeurobasalTM medium with B-27TM Plus Supplement was used to maintain standard primary rat neuronal cultures until iron exposure ...
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bioRxiv - Microbiology 2024Quote: ... Human iPSCs were obtained from STEMCELL Technologies (SCTi003- A). A549 ...
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bioRxiv - Immunology 2024Quote: ... 100 ng/mL IL-6 (both from STEMCELL Technologies), 50 ng/mL TNFα (eBioscience ...
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bioRxiv - Immunology 2024Quote: Commercial frozen human PBMCs were purchased from StemCell Technologies. HG002 EBV-LCL were purchased from Coriell Institute of Medical Research and expanded for 14 days in Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells were extracted from blood drawn from healthy donors using density gradient separation (Lymphoprep, Stem Cell Technologies, Vancouver, BC, Canada) and cultured at 1 × 106 cells/ml in RPMI complete medium containing 1μg/ml Concanavalin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Whole bone marrow from transplanted mice or HSPCs from Dnmt3a+/+ or Dnmt3aR878H/+ mice were plated in MethoCult GF M3434 (StemCell Technologies cat. #03434) at the indicated numbers and cultured at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...