Labshake search
Citations for Stemcell Technologies :
351 - 400 of 726 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Bioengineering 2023Quote: ... 2.5 g of Hydrosil silicone components (1:1 wt) (101301; SILADENT) was added into each well of 6-well microwell culture plate (AggreWellTM400; STEMCELL Technologies) followed by centrifugation at 55 x g for 1 min and curing at 60 °C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Bioengineering 2023Quote: ... gift from the Seidman Lab) or GFP-DSP reporter (WTC; Allen Institute AICS-0017 cl.6) were cultured in mTeSR1 media (StemCell Technologies) on Matrigel (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... the media was changed to remove the ROCK inhibitor and was maintained for 6 hours in in mTeSR plus (Cat# 100-0276, StemCell Technologies). The media was then replaced with DMEM/F12 (Cat# 11320033 ...
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bioRxiv - Bioengineering 2024Quote: Our protocol began with cultivating human pluripotent stem cells (H9) in a Matrigel-coated 6-well plate using a stem cell feeder-free culture medium (mTeSR1, STEMCELL Technologies). For routine passaging ...
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bioRxiv - Cell Biology 2024Quote: ... The microglial differentiation was performed on thawed HPCs (200,000 cells per well of a 6-well plate) using the STEMdiffTM Microglia Culture System (cat. 100-0019 and 100-0020, STEMCELL Technologies) for 24 days according to manufacturer’s directions ...
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bioRxiv - Immunology 2023Quote: ... PBMC were isolated from blood collected in ethylenediaminetetraacetic acid-anticoagulated (EDTA) tubes by lymphocyte separation medium density gradients (Stemcell technologies, cat# 07801) and resuspended in PRMI 1640 medium supplemented with 10% FCS ...
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bioRxiv - Systems Biology 2020Quote: ... followed by trituration in 5 U/mL dispase (Stem Cell Technologies 07913) plus 1 mg/mL DNase I (Stem Cell Technologies 07900 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Cancer Biology 2020Quote: 5 × 105 nucleated splenocytes were cultured with Methocult M3434 medium (STEMCELL Technologies) supplemented with human thrombopoietin (50 ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies, Catalog# 05701), 50 μL of 20 μg/ml EGF (Peprotech Inc ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/mL recombinant human IL-15 (StemCell Technologies, Cat # 78031). Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 5 nM Y-27632 dihydrochloride ROCK inhibitor (Stem Cell Technologies). The iPSCs were subsequently fed daily for 3 consecutive days with Neuronal Induction Medium (DMEM/F12 medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with Hydrocortisone stock solution (5 μl/ml; Stem cell technologies #07925) and Heparin solution (2 μl/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 5×102 HSPCs were seeded in 1.5ml of Methocult Optimum (STEMCELL Technologies) and after 14 days ...
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bioRxiv - Microbiology 2024Quote: ... medium was replaced by ODM supplemented with 5 µM CHIR99021 (StemCell technologies) and 10 µM Y-27632 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µM (for iPSC) or 5 µM (for ESC) XAV939 (Stemcell Technologies), and 10 µM Y27632 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were passaged using a 5 min incubation with Accutase (StemCell Technologies) on Matrigel-coated plates in mTeSR1 media supplemented with 10 μM of Y-27632 (Cayman Chemicals) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were further dissociated in Dispase 5 U/mL (STEMCELL Technologies, 07913) plus 50 µg/mL DNase I (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was supplemented with 5 or 10mM CHIR99021 (Stemcell technologies 72052), or with 200 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD38-FITC (clone AT1; 5 μL; cat. # 60131FI) from STEMCELL Technologies; and anti-CXCR5-PE (clone MU5UBEE ...
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bioRxiv - Immunology 2024Quote: ... incubated for 5 min with 125 µL/mL RapidSphere beads (StemCell Technologies), then volume topped up to 2.5 mL and placed in EasySep magnet for 2.5 min ...
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bioRxiv - Cell Biology 2024Quote: ... followed by basic medium containing 5 μM IWR-1-endo (STEMCELL Technologies) for another 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... B27GB medium was supplemented with 5 ng/mL GDNF (78058, Stemcell Technologies) and 5 ng/mL BDNF (ref ...
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bioRxiv - Cell Biology 2024Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were fed daily and split every 4-7 days using ReLeSR (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Genetics 2021Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... after which 4 ml maturation medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) were gently added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...