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Citations for Stemcell Technologies :
3751 - 3800 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... hPSCs were maintained under feeder-free conditions in complete mTeSR Plus medium (Stem Cell Technologies) and passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 80% confluent iPSC were dissociated into a single-cell suspension using Accutase (StemCell Technologies) and plated on Matrigel-coated plates (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... CD4+ T cells were immunomagnetically purified by negative selection according to manufacturer’s instructions (STEMCELL Technologies). Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... in embryoid body (EB) Seeding medium with 50 μM ROCK inhibitor (Y27632, Stem Cell Technologies). On day 7 EBs were transferred from AggrewellTM800 24-well plates using wide-bore p1000 pipette tips and embedded in Matrigel by gently mixing around 30 EBs in 100 μl Expansion medium with 150 μl cold Matrigel (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... hESCs were maintained on geltrex/matrigel coated plates in mTeSR plus medium (Stem Cell Technologies) in a cell culture incubator under low oxygen conditions (95% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation using Lymphoprep (STEMCELL Technologies). Cell viability was assessed using 0.4% Trypan Blue solution (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... and passaged every 3-4 days using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) for clump passaging ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 µM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 50 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Stem Cell Technologies). The cell suspension was distributed into ultra-low attachment 96-well plates (Corning) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were differentiated using mouse MesenCult™ Adipogenic and Osteogenic Differentiation Kits from StemCell Technologies. Cells were differentiated for 3 weeks and then fixed ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were split using Accutase (Merk) and cells were seeded with Rock Inhibitor (STEMCELL Technologies) (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: Cholangiocyte organoids were subjected to a 15-day differentiation protocol developed by STEM CELL Technologies (“Initiation ...
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bioRxiv - Neuroscience 2023Quote: ... Pluripotency tests of iPSC lines using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies, 05230) according to manufacturer instructions demonstrated differentiation capacity of all three germ layers (Fig ...
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bioRxiv - Neuroscience 2023Quote: Later differentiations also utilized the kit developed based on this protocol (Stem Cell Technologies, 08620) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Preparation and activation of papain solution was performed as per the instructions (Stem Cell Technologies). One ml of papain solution per organoid per well in a 12-well plate was used ...
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bioRxiv - Neuroscience 2024Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
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bioRxiv - Immunology 2024Quote: ... Peripheral mononuclear blood cells (PBMCs) were collected using SepMate-50 PBMC Isolation Tubes (STEMCELL Technologies) and frozen slowly at -80 C before transfer to liquid nitrogen for long-term storage ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated for 7 days submerged in PneumaCult-ExPlus (STEMCELL Technologies, Vancouver, BC, Canada). After the 7-day culture ...
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bioRxiv - Physiology 2024Quote: ... the cell-laden tubes were transferred to Pneumacult™ Airway Organoid Seeding Medium (Stemcell Technologies) at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were visualized at day 7 or 14 using the STEMVision colony counter (STEMCELL Technologies) with manual counting to ensure accuracy.
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bioRxiv - Immunology 2024Quote: ... were purified by negative selection using RosetteSep B-cell enrichment Cocktail (StemCell Technologies, Vancouver, Canada) followed by density gradient centrifugation on Lympholite (Cedarlane Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... CD34+ cells were then plated onto MS5 monolayer in MyeloCult H5100 media (Stemcell Technologies, #05150) and cells treated with 0.5µM AZA for 4 consecutive days ...
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bioRxiv - Molecular Biology 2024Quote: ... or 1000 MDS-L cells were seeded per 1mL of MethoCult H4434 (StemCell Technologies, #04434) in 35mm dishes ...
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bioRxiv - Synthetic Biology 2024Quote: ... CD34+ HSPCs were cultured at 1-5×105 cells/mL in StemSpan SFEMII (STEMCELL Technologies) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Immunology 2024Quote: ... The tissue segments were incubated at 37°C with Gentle Cell Dissociation Reagent (Stemcell Technologies) for 5 minutes with shaking at 100 rpm ...
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bioRxiv - Immunology 2024Quote: ... Annexin V positive dead or dying cells were depleted using immunomagnetic negative selection (STEMCELL technologies).
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bioRxiv - Neuroscience 2024Quote: ... H9 hPSCs (WA-09, WiCell) were cultured in mTeSR1 media (Stem Cell Technologies, Vancouver, Canada) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... BM cells (105) were aliquot in 1 mL of Iscove’s modified Dulbecco’s medium (StemCell Technologies #07700) then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... WBM (5×104 cells) were plated in duplicate wells of Methocult GF M3434 methylcellulose (StemCell Technologies) according to the manufacturer’s suggestions ...
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bioRxiv - Cell Biology 2020Quote: ... Cerebral organoids were generated and grown using the STEMdiff Cerebral Organoid Kit (Stem Cell Technologies, 08570) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: hPSCs were first differentiated into definitive endoderm using the STEMDiff Definitive Endoderm Kit (Stem Cell Technologies), with 1 day addition of supplement CJ and MR and 2 days addition of supplement CJ only ...
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bioRxiv - Immunology 2022Quote: ... and cryopreserving them at 30 million/mL in serum-free expansion medium (SFEM; Stem Cell Technologies) containing 5% fetal bovine serum and 10% dimethylsulfoxide (DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... On day -1 cells were plated into mTeSR1 medium with 0.5 μM Thiazovivin (STEMCELL technologies # 72252) onto hESC-matrigel coated (Corning # 354277 ...
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bioRxiv - Cell Biology 2022Quote: The CFU assay was performed according to the manufacturer’s instruction of Methocult H4435 (Stem Cell Technologies). Firstly ...
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bioRxiv - Physiology 2019Quote: ... Single cell suspensions were seeded on plates coated with 10µg/ml Vitronectin XF™ (STEMCELL Technologies) in maintenance media supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem ...
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bioRxiv - Immunology 2019Quote: Monocytes were enriched from the same leukopoiesis products as T-cells using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4 ...
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bioRxiv - Genetics 2020Quote: ... Cryopreserved CD34+ cells were thawed and pre-stimulated for 48 h in StemSpan SFEM (StemCell Technologies) supplemented with 100 ng/mL SCF ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with Epo alone at 0.5 U/mL or complete methylcellulose media H4435 (Stem Cell Technologies) at 200 cells/mL ...
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bioRxiv - Developmental Biology 2019Quote: Frozen Umbilical cord blood (UCB) derived CD34+cells were purchased from Stemcell Technologies (Cat No. 70008.5) from consenting donors with protocols approval by either the Food and Drug Administration (FDA ...
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bioRxiv - Cancer Biology 2019Quote: ... CD4+ or CD8+ T cell enrichment was performed using magnetic beads (Miltenyi Biotec or STEMCELL Technologies). Naïve CD4+ T cells were isolated using a naive CD4+ T cell isolation kit (Miltenyi Biotec).
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bioRxiv - Microbiology 2020Quote: ... monocytes and others (CD4− CD8− CD19− cells) using CD4 negative selection kit (STEMCELL Technologies, https://www.stemcell.com), CD8 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... The prepared cells (in 15 mL centrifuge tubes) were placed in EasySep magnets (Stemcell Technologies 18001) which retained labeled cells during washes ...
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bioRxiv - Cell Biology 2019Quote: ... Then a warm solution containing 20 µM blebbistatin (final concentration in cell medium; Stemcell Technologies, 72402) or DMSO (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... T-cells were isolated with an EasySep CD3+ enrichment kit from StemCell Technologies (Vancouver, BC Canada), per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The B cells were isolated from fresh blood samples using negative selection kits from Stemcell Technologies (EasySep Direct Human B Cell Isolation Kit) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were thawed the day of use and kept in StemSpan SFEM II media (StemCell Technologies) with CD34 supplement (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cerebral and ocular organoids were cultured in mTeSR1 medium (Stem Cell Technologies, cat. no. 85850). Human ES cell (H1 WA01 and H9 WA09 ...
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bioRxiv - Synthetic Biology 2021Quote: ... And then single cell clones were first detached using 20 μl Accutase (STEMCELL Technologies cat# 07920) and neutralized with 20 μl growth media ...
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bioRxiv - Molecular Biology 2020Quote: ... provided by Walters et al37,70 were expanded in PneumaCult-Ex Plus Basal Medium (Stem Cell Technologies) supplemented with Pneumacult Ex Plus supplements (Stem Cell Technologies) ...