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Citations for Stemcell Technologies :
301 - 350 of 1973 citations for Mouse Stress Induced Phosphoprotein 1 STIP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Bioengineering 2023Quote: The Mouse IntestiCult medium (STEMCELL Technologies) was prepared and supplemented with 100 U/mL Pen/Strep (Gibco) ...
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bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: Mouse hepatic progenitor organoids (70932, STEMCELL Tech) were seeded in 96 well plate using 20ul Matrigel (Corning ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... in mouse Mesencult Expansion medium (StemCell Technologies). After 10 days in culture ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Neuroscience 2020Quote: ... plus mouse NeuroCult proliferation supplements (Stem Cell Technologies) added with 2 µg/ml heparin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: IntestiCult Organoid Growth Medium (mouse) (Stem Cell Technologies) was used to establish and culture mouse intestinal organoids ...
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bioRxiv - Microbiology 2021Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
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bioRxiv - Cell Biology 2020Quote: ... we added IntestiCult Mouse Organoid Growth Medium (STEMCELL Technologies) to the wells ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse methylcellulose complete media (Stem Cell Technologies, Vancouver, BC) was used to assay hematopoietic colony formation ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Cell Biology 2021Quote: ... using Mouse IntestiCult™ Organoid Growth Medium (Stemcell Technologies #06005) supplemented with 100 mg/ml ampicillin (Sigma #A0166 ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Mouse NeuroCult basal medium (Stem Cell Technologies #05700) without growth factors ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse methylcellulose complete media (M3434, Stem Cell Technologies, Vancouver, BC) were used to assay hematopoietic colony formation ...
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bioRxiv - Systems Biology 2024Quote: ... Mouse and Rat proliferation supplement 10% (Stem Cell Technologies #05701), Recombinant Human FGF (20 ng/mL ...