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Citations for Stemcell Technologies :
301 - 350 of 1499 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Dendritic cells (DCs) were isolated from spleens of wild type mice using EasySep™ Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies, 19863). Co-cultures were set up in 4 replicates with BMDM primed as above ...
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bioRxiv - Immunology 2024Quote: ... OT-I T cells (CD8+) were isolated from spleens of OT I mice using EasySep™ Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies, 19853A). Dendritic cells (DCs ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: T cells were extracted from OT-II mouse spleens and magnetically sorted using a T-cell extraction kit from Stem Cell Technologies (catalog number 19851). Cells were incubated in culture media (RPMI+ 10% HIFBS + Pen/Strep + 1 mM HEPES + 50 μM beta mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ILC enriched samples were preserved in PIPseqTM T100 3L Single Cell Capture and Lysis Kit and processed by sequencing service (Fluent BioSciences ...
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bioRxiv - Biochemistry 2022Quote: Mouse hepatic progenitor organoids (70932, STEMCELL Tech) were seeded in 96 well plate using 20ul Matrigel (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Nestin (STEMCELL Technologies; 1:100); Rabbit anti-GFAP (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg/ml mouse heparin (Stemcell technologies), 1X Pen/strept (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... in mouse Mesencult Expansion medium (StemCell Technologies). After 10 days in culture ...
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bioRxiv - Neuroscience 2020Quote: ... plus mouse NeuroCult proliferation supplements (Stem Cell Technologies) added with 2 µg/ml heparin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Beta Tubulin (STEMCELL Technologies; 1:500); Rabbit anti-Olig2 (PhosphoSolutions ...
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bioRxiv - Immunology 2023Quote: IntestiCult Organoid Growth Medium (mouse) (Stem Cell Technologies) was used to establish and culture mouse intestinal organoids ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were kept in MEF medium for 48 h and then the medium was changed to mouse embryonic stem cell (mESC) medium: DMEM with the addition of 15% mouse ESC maintenance FBS (STEMCELL Technologies), 100 units ml−1 ESGRO mouse leukaemia inhibitory factor (mLIF ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 nM bone morphogenetic protein 4 (BMP4, Stemcell technologies) was added to the medium for another three days ...
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bioRxiv - Cell Biology 2020Quote: ... we added IntestiCult Mouse Organoid Growth Medium (STEMCELL Technologies) to the wells ...
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bioRxiv - Genetics 2022Quote: ... Anti-nestin mouse antibody (StemCell Technologies, 60091, 1:1000), Alexa Fluor 488-anti-Mouse (A11029 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse methylcellulose complete media (Stem Cell Technologies, Vancouver, BC) was used to assay hematopoietic colony formation ...
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bioRxiv - Cell Biology 2021Quote: ... using Mouse IntestiCult™ Organoid Growth Medium (Stemcell Technologies #06005) supplemented with 100 mg/ml ampicillin (Sigma #A0166 ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Mouse NeuroCult basal medium (Stem Cell Technologies #05700) without growth factors ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse methylcellulose complete media (M3434, Stem Cell Technologies, Vancouver, BC) were used to assay hematopoietic colony formation ...
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bioRxiv - Systems Biology 2024Quote: ... Mouse and Rat proliferation supplement 10% (Stem Cell Technologies #05701), Recombinant Human FGF (20 ng/mL ...
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bioRxiv - Neuroscience 2020Quote: ... CGM was constituted of mouse NeuroCult basal medium (Stem Cell Technologies) plus mouse NeuroCult proliferation supplements (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 µl of MammoCult™ Organoid Growth Medium (Mouse, STEMCELL Technologies) + Gentamicin (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... in IntestiCult Organoid Growth Medium (Mouse) (Stem Cell Technologies, cat.# 06005) supplemented with 1% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... anti-GYPA-FITC clone 2B7 mouse monoclonal (Stem Cell Tech 60152FI), anti-GYPC-FITC mouse monoclonal (IBGRL) ...
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bioRxiv - Biochemistry 2023Quote: ... conjugated to the EasySepTM Mouse Streptavidin RapidSpheresTM (Cat: #19860A, STEMCELL Technologies), and separated on the EasySepTM Magnet (Cat:#18000 ...
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bioRxiv - Neuroscience 2023Quote: ... matrix protein coated plates and cultured in mTeSR™ medium (STEMCELL tech), passaged using Versene (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with MesenCult™ adipogenic stimulatory supplement for mouse (Stem Cell Technologies) and 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with MesenCult™ adipogenic stimulatory supplement for mouse (Stem Cell Technologies) and 2 mM L-glutamine for another 14 days ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies, Catalog# 05701), 50 μL of 20 μg/ml EGF (Peprotech Inc ...
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bioRxiv - Neuroscience 2021Quote: ... 1x Mouse Proliferation Supplement (#5701) in NeuroCult Media (#5702 Stem Cell Tech). The dissociated cells were then grown as floating neurospheres in proliferation media at 37⁰C and 5% CO2 ...
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bioRxiv - Genomics 2021Quote: ... Antibodies used in immunofluorescence: Anti-Mouse OCT4 (STEMCELL TECHNOLOGIES, 60059, 1:200); Rabbit Monoclonal Anti-Sox9 (abcam ...
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bioRxiv - Immunology 2023Quote: ... and grown with IntestiCult™ Organoid Growth Medium (Mouse) (Stem Cell Technologies). Organoids embedded in Matrigel were cultured on multi well chambers After the growth plus/minus treatments the chambers were washed and TRIzol (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... DIPG4423 cells were cultured in Mouse Neurocult media (Stem Cell Technologies #05700), Mouse and Rat proliferation supplement 10% (Stem Cell Technologies #05701) ...