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Citations for Stemcell Technologies :
301 - 350 of 601 citations for IL 3 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Cells were plated at a density of 2-3×103/cm2 in pEPSCM medium27 containing the Rho-associated coiled kinase (ROCK) inhibitor Y-27632 (5μM, Stemcell Technologies, #72304). Cells were fed the following day with pEPSCM without Y-27632 then fed daily ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cell Biology 2019Quote: ... Hematopoietic stem cells were enriched using the EasySep Mouse Hematopoietic Progenitor Cell Enrichment kit (Stemcell Technologies) and 1*106 HSCs were administered via tail vein injection to recipient mice ...
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bioRxiv - Immunology 2020Quote: Isolated IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were isolated using the EasySep Mouse CD4+ T-cell isolation kit (Stemcell Technologies). The CD4+ T cells were then added in a 1:1 ratio to cultured CMT167 cells ...
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bioRxiv - Immunology 2021Quote: ... S3d) for the studies involving glucose exposure using EasySep™ Mouse Neutrophil Enrichment Kit (STEMCELL Technologies), as per manufacture’s protocol and as described previously (25 ...
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bioRxiv - Genomics 2019Quote: Mouse bone marrow cells (2.5×104) suspended in methylcellulose semisolid medium (Methocult M3231) (Stem Cell Technologies) were plated in 35-mm culture dishes in the presence of 0.5 % FBS ...
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bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...
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bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
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bioRxiv - Immunology 2022Quote: B cells from spleen were sorted with EASYSEP MOUSE B cell isolation kit (Stem Cell Technologies) and lysed using RIPA buffer (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... then DCs were isolated using the EasySep™ Mouse CD11c Positive Selection Kit II (STEMCELL Technologies). While adding the CD11c selection antibody cocktail to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Lineage-negative cells were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol prior to sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... and T lymphocytes purified using Easy SepTM Mouse T cell isolation kit (STEMCELL Technologies, Cat# 19851A), as we described83 ...
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bioRxiv - Cancer Biology 2023Quote: ... HSCs were enriched using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were isolated using the EasySep Mouse CD8+ T cell isolation kit (StemCell Technologies) and resuspended at a concentration of 106 cells/mL in complete RPMI supplemented with 1X sodium pyruvate (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... in EpiCult™-B (mouse) medium supplemented with human recombinant EGF (hrEGF) (Stem Cell Technologies, 78006), human recombinant bFGF (hrbFGF ...
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bioRxiv - Immunology 2023Quote: ... Dendritic cells were isolated with the EasySepTM Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies). T Cells were isolated with the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Neuroscience 2020Quote: The human embryonic stem cell (hESC) line harbouring the GPF reporter at the 3’UTR of CRX locus was expanded in mTeSR™1 (StemCell Technologies) at 37°C and 5% CO2 on 6 well plates pre-coated with Low Growth Factor Matrigel (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
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bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Genomics 2020Quote: ... and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2019Quote: ... and NK cells were purified by negative selection using EasySep Mouse NK Cell Isolation Kit (STEMCELL TECHNOLOGIES). Normalized NK-cell numbers (1–3 × 106 ...