Labshake search
Citations for Stemcell Technologies :
301 - 350 of 512 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... 0.6 μg of a rat anti-mouse F4/80-PE antibody was incubated for 15 min at room temperature and samples were washed with 3 mL of EasySepTM buffer (ESB) (cat. no. 20144, STEMCELL Technologies), centrifuged ...
-
bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2024Quote: ... CD34+ cells were plated at a density of approximately 25,000-100,000 cells per well and spun down at 250 RPM for 3 minutes in StemSpan SFEM II media (STEMCELL Technologies, 09655). Pre-warmed media was used to resuspend the CD34+ cells ...
-
bioRxiv - Bioengineering 2022Quote: ... printed constructs were incubated for 20 min in 5 mg mL-1 collagenase type I (STEMCELL Technologies) in PBS supplemented with 5% FBS and then lysed with RLT buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... and maintained in culture medium containing mTeSR plus basal medium and 5 x supplement (Stem Cell Technologies). Thiazovivin (2 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... medium was replenished daily and were passaged every 5-6 days with Dispase I (StemCell Technologies, 07923) in small cell clumps ...
-
bioRxiv - Cancer Biology 2022Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). In the morning ...
-
bioRxiv - Neuroscience 2021Quote: ... Cultures were fed every other day and passaged every 5–7 days by ReLeSR™ (STEMCELL Technologies).
-
bioRxiv - Cell Biology 2021Quote: ... This was followed by cardiac specification using RPMI B27-containing 5 μM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Bioengineering 2023Quote: ... The iPSCs were routinely passaged every 5-7 days using ReLeSR™ Passaging Reagent (Stemcell Technologies, Canada). The iPSCs were then differentiated into SCPCs based on a modified protocol to generate spinal motor neurons (Hor et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... The EBs were cultured in the plate for 5 days with neural induction medium (Stem cell technologies) to initiate neural induction ...
-
bioRxiv - Cell Biology 2023Quote: ... This was followed by cardiac specification using RPMI B27-containing 5 μM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). On the morning ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
-
bioRxiv - Bioengineering 2023Quote: ... + 5% fetal bovine serum + 1% Pen/Strep (Mediatech, MT30-002-CI) +/- 30 µM Y27632 (StemCell Technologies, 72304) was added to just cover the dissected kidneys ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
-
bioRxiv - Cancer Biology 2022Quote: ... transduced AML cells were cultured on MS-5 cells (DSMZ) in Myelocult media H5100 (StemCell Technologies Inc.) with rhIL-6 ...
-
bioRxiv - Immunology 2024Quote: ... In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySep™ Buffer (20144; Stemcell Technologies) at a concentration of 1 x 108 cells/mL and incubated with 100 µL selection antibody cocktail per mL of sample for 10 min at RT ...
-
bioRxiv - Microbiology 2024Quote: ... the medium was replaced with RPMI 1640 containing 5 μM XAV-939 (Stem Cell Technologies, Cat.#72674), 500 μg/mL BSA ...
-
bioRxiv - Cell Biology 2024Quote: ... for the first day after passages and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies). The pluripotency of iPS cells were characterized by immunostaining with OCT3/4 ...
-
bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
-
bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Neuroscience 2020Quote: The human embryonic stem cell (hESC) line harbouring the GPF reporter at the 3’UTR of CRX locus was expanded in mTeSR™1 (StemCell Technologies) at 37°C and 5% CO2 on 6 well plates pre-coated with Low Growth Factor Matrigel (Corning) ...
-
bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
-
bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
-
bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
-
bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
-
bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
-
bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
-
bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
-
bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
-
bioRxiv - Cancer Biology 2024Quote: ... Spheroid formation media was made in a 3:1 ratio of cell culture media:MethoCult H4100 (StemCell Technologies, Cat#04100, Cambridge, MA, USA), and the total volume was calculated 100µL per well ...
-
bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
-
bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
-
bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10ng/ml bFGF and 5 ng/ml BMP4 and plated in ultra-low attachment plates (Stem Cell Technologies). Two days later ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
-
bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
-
bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...