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Citations for Stemcell Technologies :
301 - 350 of 1231 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cells at week 3 of terminal differentiation were switched to BrainPhys medium (STEMCELL Technologies), and experiments were conducted after a total of 5–6 weeks of differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented for 3 days with 10 μM Rock inhibitor Y-27632 (StemCell Technologies, 78003).
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in the presence of 3 μM CHIR-99021 (Stem Cell Technologies). Then cells were cultured for 1 day without cytokines ...
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bioRxiv - Immunology 2024Quote: ... 3 ml of prewarmed collagenase type IV (1mg/mL) digestion medium (StemCell Tech 07909) was added to cell pellet ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
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bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated into single cells and clustered in AggreWell 400 6-well plates (Cat. No. 34425, STEMCELL Technologies) with DMEM + 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... and plated into a poly-hema treated 6-cm Petri dish in media containing mTeSR1 (StemCell Technologies, Cambridge, MA) and 10μM rock inhibitor (RI ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
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bioRxiv - Cell Biology 2024Quote: ... The hiPSCs were maintained on Corning Matrigel-coated 6-well tissue culture plates with mTeSR-Plus medium (STEMCELL Technologies). Cells were passaged every 4 days using ReLeSR media (StemCell Technologies ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... 480 ng/mL hydrocortisone and 4 µg/mL heparin (STEMCELL Technologies, 07980). ALI cultures were grown in a 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... PneumaCult ALI medium supplemented with 4 μg/mL heparin (Stem Cell Technologies), 5 μM hydrocortisone ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of 29% Iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were plated on 6-well plates coated with geltrex (Life Technology) and cultured in TeSR-E8 medium (Stemcell Technology). For hiPSC-CMs differentiation ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were seeded on pre-treated (0.167 mg/mL Matrigel) 6-well plates and cultured in TeSR-E8 iPSC culture medium (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... seeded in PLO/Lm coated 6-well plates (EBs produced in one well of AggreWell™ 400 24-well plate were usually seeded into one well of a 6-well plate) and maintained in AAN2 medium (0.5% N2 Supplement (ref.07152, Stemcell Technologies), 200 mM AA-ascorbic acid (ref.12084947 ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were pre-coated with coated with 3 μg/ml of vitronectin (07180, Stemcell Technologies) and 5mg/mL Col IV (C7521-5MG ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 3 million cells were transferred to each well in AggreWell 800 (STEMCELL Technologies, 34815) and centrifuged at 100g for 3 minutes in hiPSC medium supplemented with ROCK inhibitor Y-27632 (EMD Chemicals) ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 × 106 cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8™ Flex Media supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Neuroscience 2024Quote: ... 3×106 iPSCs were seeded into a well of an AggreWell 800 plate (STEMCELL Technologies) to form EBs (Haenseler et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded at 5000 cells/ml in 3 ml MethoCultTM (Stem Cell Technologies, H4034) in a 3.5 cm culture dish and placed in an incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... at 8.8µg/cm2 and passaged every 4 days using ReLeSR (Stem Cell Technologies). All hPSCs experiments were conducted with cells ranging from 40 and 70 passages ...
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bioRxiv - Developmental Biology 2021Quote: ... and cells were routinely passaged every 4 days using ReleSR (StemCell Technologies; 05872). CRISPRi-SOX2 cells were plated on vitronectin-coated (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... 4 million hiPSC were seeded into an AggreWell 800 well (Stem Cell Technologies) to generate embryoid bodies (EBs ...
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bioRxiv - Molecular Biology 2023Quote: ... Every 4-5 days cells were passaged using ReLeSR reagent (Stemcell tech, 05872).
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bioRxiv - Molecular Biology 2024Quote: ... inhibition of canonical Wnt signaling is performed using IWP-4 (#72552, STEMCELL Technologies) a Wnt signaling inhibitor ...
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bioRxiv - Cancer Biology 2023Quote: ... 5,000 cells were re-suspended in 4 mL of Methocult media (STEMCELL Technologies). 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Undifferentiated iPSCs were detached by a 4-min incubation with Accutase (StemCell technologies) and seeded onto Geltrex (Life Technologies)-coated plates at a density of 200,000 cells/cm2 ...
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bioRxiv - Neuroscience 2024Quote: ... EBs were cultured for 4 days in mTeSR™ Plus medium (STEMCELL Technologies), supplemented daily with 50 ng/mL bone morphogenetic protein 4 (BMP4) ...
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bioRxiv - Cancer Biology 2024Quote: ... edited residual HSCs were added to 4 mL of MethoCult H4435 (Stemcell Technologies) and plated in triplicate ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...