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Citations for Stemcell Technologies :
251 - 300 of 398 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and then incubated in a digestion buffer of 5 Units/mL dispase (Stemcell Technologies, #07913) and 0.1 mg/mL DNase I (Worthington ...
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bioRxiv - Immunology 2021Quote: ... The pellet was washed twice each time with 20 mL of EasySep buffer (STEMCELL Technologies) followed by centrifugation at 400 rcf for 10 min with brakes on ...
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bioRxiv - Immunology 2021Quote: ... PBMC were obtained from fresh blood samples by density gradient centrifugation using Lymphoprep (Stemcell Technologies) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from human blood samples were isolated on a density gradient medium (Lymphoprep, STEMCELL Technologies). Cells were incubated 4°C for 10 min with anti-mouse or anti-human CD16/CD32 (BD Pharmingen ...
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bioRxiv - Microbiology 2024Quote: ... isolated from fresh blood samples using a RosetteSep Human NK Cell Enrichment Cocktail (StemCell Technologies), were infused into each lane ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Immunology 2021Quote: ... inguinal lymph nodes from immunized or naïve mice or live LN sections were incubated in 1 mL of digestion solution comprised of RPMI1640 medium containing 0.8 U/mL Dispase (07913, Stemcell Technologies), 0.1 mg/mL Collagenase D (11088858001 ...
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bioRxiv - Biophysics 2021Quote: ... the beads were removed carefully and the agarose wells were coated with a non-adhesive coating solution (07010, Stemcell Technologies) for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
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bioRxiv - Bioengineering 2024Quote: ... each well of the 96 well plate was first coated with 45 μL 2.25% Type I Bovine Collagen Solution (StemCell Technologies) for 1 hour at 37 C ...
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bioRxiv - Bioengineering 2023Quote: ... and broken down to small fragments by a 200uL pipet tip rinsed with Anti-Adherence Rinsing Solution (07010, STEMCELL Technologies). The tissue fragment suspension was transferred into an Anti-Adherence Rinsing Solution coated 15mL conical tube on ice and allowed to sediment by gravity for at least 5 minutes ...
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bioRxiv - Genomics 2022Quote: Removal of red blood cells was done by treating cell pellets with cold ammonium chloride solution (Stem Cell Technologies, 07850) diluted with 1 part PBS-HF ...
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Immunology 2024Quote: ... spleens were mechanically dissociated by a syringe plunger between two pieces of mesh to create a single cell suspension followed by red blood cell lysis by incubation in 0.8% ammonium chloride solution (STEMCELL Technologies) and subsequent staining ...
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bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Neuroscience 2024Quote: ... The spheroid-forming wells were prepared prior to cell seeding by adding 0.5 mL of anti-adherence rinsing solution (07010, STEMCELL Technologies) to each well ...
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bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 2.5 ml of FACS buffer and isolated on an EasySep magnet (Stemcell Technologies, #18000). The magnet-bound fraction was collected ...
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bioRxiv - Immunology 2024Quote: ... and 1.78x PBS equilibration buffer and the EasySep Direct Human Neutrophil Isolation kit (StemCell Tech #19666). The kits were used as suggested by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Bioengineering 2024Quote: ... non-TC treated 96-well U-bottom plates were passivated with 100 μL anti-adherence rinsing solution (AAR, StemCell Technologies 07010) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 cells were plated in 200 μL media in low-adhesion plates coated with Aggrewell Antiadherence solution (Stem Cell Tech #07010) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were passaged with Versene solution (Paneco, Russia) at a ratio of 1:15 – 1:20 every 4–5 days with ROCK-inhibitor (StemCell Technologies).
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bioRxiv - Bioengineering 2021Quote: ... The B cells were isolated from fresh blood samples using negative selection kits from Stemcell Technologies (EasySep Direct Human B Cell Isolation Kit) ...
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bioRxiv - Cancer Biology 2022Quote: ... primary patient samples and healthy controls were plated in methylcellulose (Stem Cell Technologies H4435, Vancouver, CA) with the CA-4948 and control ...
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bioRxiv - Developmental Biology 2023Quote: ... and samples were pooled prior to CD34+ cell isolation using the MACS separation system (Stemcell Tech).
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bioRxiv - Immunology 2024Quote: ... samples were enriched for bead-bound cells on magnetized columns (StemCell Technologies #17684 and #17681, respectively). Samples then underwent surface staining on ice with antibodies as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples underwent positive CD34 selection using EasySep Human CD34 positive Selection kit II (Stem Cell Technologies).
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bioRxiv - Immunology 2024Quote: ... In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySep™ Buffer (20144; Stemcell Technologies) at a concentration of 1 x 108 cells/mL and incubated with 100 µL selection antibody cocktail per mL of sample for 10 min at RT ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were mechanically detached in VP spheroid medium using a serological pipette before transfer to a 6WP pre-coated with anti-adherence solution (STEMCELL Technologies, 07010). Detached cells from five wells of a 6WP were added divided over six wells of a 6WP ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...