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Citations for Stemcell Technologies :
251 - 300 of 1346 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ and CD8+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ or CD8+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2024Quote: ... by using EasySep® Human Naïve CD4+ T Cell Isolation Kit II and human memory CD4+ T cell enrichment kit respectively (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: We isolated CD8+ T cells from primary human donor PBMCs using the EasySep Human CD8+ T cell Isolation Kit (STEMCELL Technologies) and activated them overnight using anti-CD3/CD28 DynaBeads (Thermo Fisher) ...
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bioRxiv - Bioengineering 2024Quote: ... and purified using either the EasySep Human CD4 T cell Isolation kit or EasySep Human T cell isolation kit to a purity of 95 % (STEMCELL Technologies, Canada ...
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bioRxiv - Immunology 2021Quote: Human NK were isolated from discarded Leukopaks from healthy volunteer blood donors using the RosetteSep™ human NK enrichment protocol (StemCell Technologies), followed by Ficoll (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The plates were incubated at 37°C for 20 min to allow the domes to solidify after which 750 μl of human intestinal organoid growth media (Human IntestiCult Organoid Growth Medium, hIOGM, StemCell Technologies, 06010) was added along with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Immunology 2024Quote: Human T cells were isolated from healthy donors who provided informed consent using RosetteSep Human T-Cell Enrichment Cocktail (STEMCELL Technologies, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... These GBM cell lines were maintained as BTICs in human neural stem cell (NSC) media containing NeuroCult NS-A basal medium (human, STEMCELL Technologies, #05750), supplemented NeuroCult NS-A proliferation kit (human ...
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bioRxiv - Microbiology 2023Quote: ... Human CD34+ cells were isolated from cord blood using EasySep™ human cord blood CD34 positive selection kit II (STEMCELL Technologies #17896), which were then cryopreserved in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from human peripheral blood using EasySepTM Human CD8+ T Cell Isolation Kit (Stemcell Technologies, Vancouver, BC, Canada) and stained with 10 μM CellTraceTM Violet Cell Proliferation Kit (VPD ...
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bioRxiv - Immunology 2024Quote: ... enriched CD8+ T cells were further purified using EasySep™ Human Naïve CD8+ T Cell or EasySep™ Human Memory CD8+ T Cell Isolation Kits (StemCell Technologies), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and the culture media were changed for 21-60 days to neural differentiation media composed by human Neurocult basal media supplemented with human Neurocult differentiation supplement (#05752, Stem Cell Technologies), 2% B-27 with vitamin A (#17504044 ...
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bioRxiv - Cell Biology 2020Quote: ... The obtained hiPSC clones were grown in mTeSR1 medium (STEMCELL Technologies), on CellMatrix Basement Membrane Gel (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... and clones were banked in mFreSR freezing medium (StemCell Technologies, 05854).
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bioRxiv - Neuroscience 2024Quote: ... in mTeSR-Plus medium supplemented with Clone R (Stem Cell Technologies). Seven to 10 days later ...
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bioRxiv - Cancer Biology 2022Quote: RosetteSep™ Human Monocyte Enrichment Cocktail (Stemcell Technologies, Canada) was used to purify human monocytes from buffy coats following manufacturer specifications ...
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bioRxiv - Pathology 2022Quote: Human biopsies were placed in PneumaCult medium (Stemcell Technologies) and sent for infection immediately ...
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bioRxiv - Cancer Biology 2022Quote: ... Human HSPCs were expanded in StemSpan SFEM (StemCell Technologies) with 1% streptomycin/penicillin ...
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bioRxiv - Cell Biology 2020Quote: Human CB CD34+ cells were purchased from STEMCELL Technologies GmbH (Cologne ...
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bioRxiv - Immunology 2020Quote: ... and the EasySep human monocyte isolation kit (StemCell Technologies) with equivalent results ...
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bioRxiv - Biochemistry 2022Quote: ... with EasySep Human T cell Isolation Kit (STEMCELL Technologies). Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies) ...
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bioRxiv - Genomics 2024Quote: Human iPSCs were maintained in mTeSR1 (StemCell Tech, 85850) or mTeSR Plus (StemCell Tech ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purchased from StemCell Technologies. Viability was assessed via DAPI staining ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD28 (ImmunoCult human T cell activator, StemCell Technologies) in total volume of 200 μl complete RPMI medium in a 96-well U bottom plate in the presence of either DMSO or soquelitinib for 48 hours ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained from Stemcell Technologies (Vancouver, BC). Samples were collected from donors and anonymized prior to providing for research purposes ...
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bioRxiv - Microbiology 2023Quote: ... The RosetteSep™ Human Monocyte Enrichment Cocktail (STEMCELL Technologies) was used to purify monocytes from the PBMCs ...
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bioRxiv - Cell Biology 2023Quote: ... and analyzed by STEMvision™ Human (Stem Cell Technologies).
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bioRxiv - Immunology 2023Quote: Frozen human PBMCs from 5 healthy donors (Stemcell Technologies, Cat# 70025.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with MesenCult MSC Stimulatory Supplement (Human) (Stemcell Technologies). One thousand viable cells/4 ml of supplemented media were plated in each well of a Costar Ultra-Low Attachment 6-well plate (Corning) ...
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bioRxiv - Microbiology 2024Quote: ... Human iPSCs were obtained from STEMCELL Technologies (SCTi003- A). A549 ...
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bioRxiv - Cancer Biology 2024Quote: ... EasySep™ Human B Cell Isolation Kit (STEMCELL technologies) were placed in transwell inserts for 6 hours at 37°C in CO2 chamber ...
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bioRxiv - Immunology 2024Quote: Human peripheral blood pan T-lymphocytes (#70024.1, StemCell Technologies) were thawed and cultured in the aforementioned T-lymphocyte media ...
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bioRxiv - Immunology 2024Quote: Commercial frozen human PBMCs were purchased from StemCell Technologies. HG002 EBV-LCL were purchased from Coriell Institute of Medical Research and expanded for 14 days in Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Human peripheral blood pan T cells (STEMCELL Technologies #70024)
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bioRxiv - Cell Biology 2022Quote: ... recombinant Activin-A (10 ng/ml) and 12 ng/ml FGF2 on 10μg/ml Vitronectin-XF (STEMCELL Technologies) or 0.1% Gelatin and MEF-medium coated plates ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... neutrophils were isolated from human whole blood based on immuno-magnetic negative isolation technique using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Cat# 19666) described in detail in (Fan et al ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: Bone marrows of sacrificed mice were extracted and human cells were separated according to expression of human CD45 (EasySep™, StemCell Technologies, Vancouver, Canada). Libraries were prepared using 10X Genomics technology (see supplemental information).
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Cell Biology 2022Quote: We isolated CD34+ cells from human cord blood and BM samples using an EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, Canada); sample purity of >95% was confirmed using flow cytometry ...