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Citations for Stemcell Technologies :
251 - 300 of 1371 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and 10 μMY27632 (Stemcell Technologies, 129830-38-2)) on day 0 ...
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bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
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bioRxiv - Neuroscience 2021Quote: ... 20ng/ml NT-3 (Stem Cell Technologies Cat # 78074), and Penicillin/Streptomycin (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 3 mL Methocult GF H4230 (Stem Cell Technologies) and plated in triplicate in 35 mm gridded plates and incubating for 8-10 d.
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bioRxiv - Immunology 2023Quote: ... and IL-3 (10 ng/mL; Stem Cell Technologies). After 5 days ...
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bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Briefly, PB was collected in FACS buffer (PBS, 2 % FBS, 2 mM EDTA) supplemented with 0.0004 % heparin solution (STEMCELL Technologies Inc.). RBCs was removed by incubation at 37 °C for 25 minutes with 1 % Dextran solution in PBS ...
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bioRxiv - Immunology 2022Quote: ... contained within either a 50 mL SepMate™ (STEMCELL Technologies) or a 50 mL conical tube ...
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bioRxiv - Microbiology 2023Quote: ... ascorbic acid (50 μg/ml, STEMCELL Technologies, Cat# ST-72132), 1 × GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled and ferumoxytol-labeled murine MSCs were tested for chondrogeneic and osteogeneic differentiation potential using MesenCult (StemCell Technologies) differentiation as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
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bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
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bioRxiv - Cancer Biology 2022Quote: ... Fibroblast Growth Factor-2 (FGF2, StemCell Technologies Cat#78134.1), Vascular Endothelial Growth Factor-A (VEGF-165 ...
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bioRxiv - Immunology 2023Quote: ... 2 million CD8 T cells (Stemcell Technologies, Cambridge, MA) from three donors were cultured for 48 hours in AIM V Medium (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... IMDM with 2% FBS (StemCell Technologies, Vancouver, BC, Canada). This HSC:MSC resuspension was added to 1 mL MethoCult H4034 Otimum (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult™ SM1 (Stemcell technologies, cat. no. 05711), 1% N-2 Supplement (Thermo Scientific ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/ml IL-3 (STEMCELL Technologies; Cat. No. 78042) was directly added to the medium to support growth in the absence of an oncogene ...
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bioRxiv - Microbiology 2022Quote: ... 20 ng/ml NT-3 (Stem Cell Technologies Cat # 78074), and Penicillin/Streptomycin cocktail ...
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bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 50 μg/ml L-ascorbic acid (L-AA, Stem Cell Technologies) and 6 μg/ml holo-transferrin (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... was dispensed to a 50 mL SepMate™ (#85450, Stemcell Technologies,) tube ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...
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bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...
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bioRxiv - Cell Biology 2020Quote: ... at 8.8µg/cm2 and passaged every 4 days using ReLeSR (Stem Cell Technologies). All hPSCs experiments were conducted with cells ranging from 40 and 70 passages ...
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bioRxiv - Developmental Biology 2021Quote: ... and cells were routinely passaged every 4 days using ReleSR (StemCell Technologies; 05872). CRISPRi-SOX2 cells were plated on vitronectin-coated (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... 4 million hiPSC were seeded into an AggreWell 800 well (Stem Cell Technologies) to generate embryoid bodies (EBs ...
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bioRxiv - Cancer Biology 2019Quote: ... and passaged every 4-5 days using Gentle Cell Dissociation Reagent (StemCell Technologies). For organoid seeding ...
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bioRxiv - Molecular Biology 2023Quote: ... Every 4-5 days cells were passaged using ReLeSR reagent (Stemcell tech, 05872).
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bioRxiv - Genomics 2023Quote: ... Undifferentiated iPSCs were detached by a 4-min incubation with Accutase (StemCell technologies) and seeded onto Geltrex (Life Technologies)-coated plates at a density of 200,000 cells/cm2 ...
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bioRxiv - Molecular Biology 2024Quote: ... inhibition of canonical Wnt signaling is performed using IWP-4 (#72552, STEMCELL Technologies) a Wnt signaling inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...