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Citations for Stemcell Technologies :
2451 - 2500 of 4689 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: Fresh or culture progeny from total BM were counted and 5000 cells/well of a 24-well plate were resuspended in Methocult (M3434, Stem Cell Technologies, Vancouver, BC, Canada). Colonies were counted at day 7 ...
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bioRxiv - Bioengineering 2019Quote: ... SVF was plated at a cell density of 7×106 in T75 tissue culture flasks in 15 ml of MesenCult MSC basal medium (Stem Cell Technologies, Cambridge, MA, USA) supplemented with MesenCult MSC Stimulatory Supplement ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
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bioRxiv - Neuroscience 2022Quote: ... At time of experimentation APCs were thawed and plated at 150,000 cell/cm2 in STEMdiff™ Astrocyte Maturation Media (Stem Cell Tech cat#100-0016) on Corning® Matrigel® coated tissue culture treated plate ...
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bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
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bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
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bioRxiv - Neuroscience 2023Quote: Astrocyte precursor cells were created from the neural progenitor cells described above using the STEMdiff Astrocyte Differentiation media kit from STEMCELL Technologies (Cat No. 100-0013) following their protocol for Matrigel-coated 6-well plates [30] ...
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bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco], 10% BIT 9500 [StemCell Technologies], and 1 mM glutamine [Gibco]) supplemented with 20ng/ml EGF (Chemicon) ...
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bioRxiv - Physiology 2020Quote: ... were then cultured in the complete L15 medium or the stem cell (SC) medium (10% human MammoCult Proliferation Supplements in MammoCult Basal Medium, Stem Cell Technologies, San Diego, CA, USA) for 7 days ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
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bioRxiv - Cancer Biology 2023Quote: ... T cells were then isolated using EasySep™ Human T Cell Isolation Kit and EasySep™ Magnet (Stemcell Technologies, Cat. No. 17951 and Cat. No. 18000) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... splenocytes in a single cell suspension were negatively selected for CD4+ or CD8+ T-lymphocytes using the EasySep Mouse CD4+ T-cell Isolation Kit or CD8+ T-cell Isolation Kit (StemCell Technologies #19852, #19853, Vancouver, BC, USA), respectively ...
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bioRxiv - Cancer Biology 2021Quote: EasySep human CD45 Depletion kit II (Stem Cell Technology, Cat#: 17898) was used to extract immune cells from freshly dissected patient tumors ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated using negative selection kits (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... or stem cell factor (20 ng/ml, STEMCELL technologies, Cat#: 78062.1), then stained with fluorescence labeled Annexin V and 7-AAD (BD apoptosis detection kit ...
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bioRxiv - Genetics 2021Quote: ... cells were cultured with 10μM ROCK inhibitor (Y-27632; STEMCELL Technologies).
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bioRxiv - Genomics 2021Quote: Expanded CB CD34+ cells were cultured in StemSpan SFEM (Stemcell Technologies) supplemented with 1X CD34+ Expansion Supplement (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10 μM Y-27632 (Stem Cell Technologies, Vancouver, Canada). The human iPSC line used in this study was the BC1 reprogrammed from peripheral blood mononuclear cells of a healthy male collected under a Johns Hopkins Institutional Review Board-approved clinical study (69).
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bioRxiv - Immunology 2020Quote: ... Freshly isolated HSPCs were resuspended in StemSpan medium (Stem Cell Technologies) supplemented with 50 ng/mL stem cell factor (SCF ...
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bioRxiv - Developmental Biology 2020Quote: ... The iPS cells were cultured in mTeSR™1 (StemCell Technologies), passaged with TrypLE Express ...
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bioRxiv - Developmental Biology 2021Quote: ... coated flat culture dish in E8 media (Stem Cell Technologies, Inc) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies) in TeSR-E8 medium (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Media was gradually switched to BrainPhys media (Stem cell technologies; # 05790) by replacing half of a well’s media twice a week ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μM TGF-β pathway inhibitor (SB431542, Stem cell technology, #72234) and ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated single cells were seeded on AggreWell 800 (#34815, StemCell Technologies). 1000 single cells were added per well in Essential 8 medium supplemented with 10μM ROCK inhibitor Y-27632 ...
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bioRxiv - Molecular Biology 2020Quote: Human cells were grown in NeuroCult medium (StemCell Technologies, Vancouver, BC) supplemented with human-EGF at 20 ng/mL and human-bFGF at 20 ng/mL supplemented with penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... recombinant human basic FGF (20ng/mL, all from Stem Cell Technologies) and 10 ng/ml laminin (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... After thawing cells were cultured in StemSpan ACF (StemCell Technologies, USA) supplemented with SCF (100 ng/ml ...
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bioRxiv - Genomics 2020Quote: ... pre-coated culture dishes and fed with mTeSR (Stem cell Technologies) supplemented with 100 I.U./ml penicillin and 100 μg/ml streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... hiPSC were maintained in mTESR plus (Stem Cell Technology, #100-0276) medium on hESC-qualified Matrigel (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... 250 µl of IntesticultTM Organoid Growth media (06005, Stem Cell Technologies) (for APCmin organoids ...
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bioRxiv - Cancer Biology 2020Quote: ... 250 µl of IntesticultTM Organoid Growth media (06005, Stem Cell Technologies) (for APCmin organoids ...
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bioRxiv - Bioengineering 2020Quote: ... Blood was then washed with RBC Lysis Buffer (Stem Cell Technologies), then centrifuged at 16 000 g for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumorspheres were dissociated and passaged using Accutase (Stem Cell Tech; # 07920). Neurosphere media was DMEM (Wisent ...
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bioRxiv - Pathology 2021Quote: ... Cells were maintained in Pneumacult ALI medium (STEMCELL Technologies, no. 05001) for 21-32 days after seeding ...
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bioRxiv - Cell Biology 2021Quote: ... in T25 flasks pre-coated with Matrigel (Stem Cell Technologies, #354277). Cells were passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 24 mM [U-13C]glucose ...
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bioRxiv - Bioengineering 2021Quote: ... to generate embryoid bodies (EBs) using E8 medium (Stem Cell Technologies) and fed daily with medium supplemented with 50 ng/mL BMP-4 ...
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bioRxiv - Bioengineering 2021Quote: ... using EasySep™ human T cell isolation kit (#17951, StemCell Technologies). 10,000 Raji cells were mixed with 100,000 T cells in media containing different concentrations of purified BiTE clones (Clone 5 and Clone 6) ...
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bioRxiv - Bioengineering 2021Quote: Pan T cells were isolated from donor PBMC’s (#70025, StemCell Technologies) using EasySep™ human T cell isolation kit (#17951 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were spun down and resuspended in BrainPhys (StemCell Technologies, 05790) supplemented with B27 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were passaged either using ReLeSR™ passaging reagent (STEMCELL Technologies) or with Accutase and cultured onto Matrigel-coated six-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... motor neuron progenitors (MEPs) were dissociated with Accutase (Stem Cell Technologies) and re-plated at 1:6 ratio on Matrigel-coated plate in neural medium supplemented with 3μM CHIR99021 (Tocris) ...
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bioRxiv - Cell Biology 2021Quote: ... coated plates in hESC or mTeSR1 medium (Stem Cell Technologies, 05850). This serial subcloning was performed until remnants of Sendai virus RNA were no longer detected and the morphology looked stable ...
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bioRxiv - Neuroscience 2020Quote: ... CGM was constituted of mouse NeuroCult basal medium (Stem Cell Technologies) plus mouse NeuroCult proliferation supplements (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2021Quote: Human peripheral blood cells (PBMCs) were purchased from STEMCELL Technologies (#70025). 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q ...