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Citations for Stemcell Technologies :
201 - 250 of 1051 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were cultured in NeuroCult™ Proliferation Medium containing 20 ng/ml epidermal growth factor (EGF) (Stemcell Technologies). For differentiation assays ...
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bioRxiv - Developmental Biology 2019Quote: ... all medium was removed and 2mL of E6 + 100 ng/mL of bFGF (Stemcell Technologies Inc. Vancouver, Canada) were added ...
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bioRxiv - Cell Biology 2023Quote: ... the hESCs were treated with liver differentiation medium (LDM) along with 50 ng/mL Activin A (STEMCELL Technologies), 50 ng/mL WNT3A (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... Electroporated cells were transferred to cRPMI medium without antibiotics containing 10 ng/mL hrM-CSF (Stem Cell Technologies) and rested at 37°C for 1 h before stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... Density gradient centrifugation was performed by transferring the blood onto 20 mL Lymphoprep solution (StemCell Technologies) and centrifugation for 30 min at 400g at room temperature (RT ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... complete solution was removed and 3 ml 2i+Lif medium + 10uM ROCK inhibitor (Stemcell Technologies, 72304) was added ...
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies, Vancouver, BC, Canada).
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bioRxiv - Cell Biology 2022Quote: ... M1 polarized cells were obtained by stimulating MΦ cells with 20 ng/mL of IFNγ (Stemcell Technologies, Cat# 78020) and 100 ng/mL of LPS for 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Genomics 2019Quote: ... Epithelium was then re-suspended in a 0.3U/ml dispase solution (07923, Stem Cell Technologies; Cambridge, MA) and incubated at 37°C for 15 min ...
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bioRxiv - Genetics 2021Quote: ... cells were cultured in R10 media additionally supplemented with 16 ng/mL of hepatocyte growth factor (HGF, Stem Cell Technologies) to preserve viability and support robust growth (Datta et al. ...
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cell Biology 2023Quote: ... Differentiation was initiated the next day with liver differentiation medium (LDM) supplemented with 50 ng/ml Activin A (STEMCELL Technologies), 50ng/WNT3A (PreproTech ...
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bioRxiv - Bioengineering 2024Quote: ... medium from the upper chamber was removed and replaced with ATDC5 medium containing 0 or 0.1 ng/ml IL-1α (STEMCELL Technologies). Following two additional days of incubation ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... placed in a stereotaxic frame and injected with either 100,000 hNSCs per side (2 μl/injection) or vehicle (2 μl HBSS with 20 ng/ml epidermal growth factor [STEMCELL Technologies, #78003] and human fibroblast growth factor [STEMCELL ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Immunology 2024Quote: ... were thawed on the day of the experiment and plated in each well supplemented with 50 ng/mL IFNγ (STEMCELL Technologies) for overnight activation ...
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bioRxiv - Cell Biology 2021Quote: ... tissue digests were thawed and placed in PBS containing 0.1mg/ml DNAse I solution (Stem Cell technologies, Cat#07900) to digest DNA released from dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... neural induction was initiated by using standard neural maintenance (N2B27) Base Media with 100 ng/ml LDN193189 (Stemcell Technologies Australia, Melbourne, Australia) and 10 µm SB431542 (Stemcell Technologies Australia ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Genomics 2021Quote: ... were sorted using a BD FACSAria™ Fusion Cell Sorter into ice-cold Serum-Free Expansion Medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies), 20 ng/ml IL-3 ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C mesoderm / Endothelial “priming” media (8 uM CHIR99021 +25 ng/ml BMP4, 1× Glutamax, 1× N2, 1× B27, Neurobasal, DMEM/F12, Stem Cell Technologies). One well of seeded cells was left untreated as negative control in StemFlex (data not shown) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
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bioRxiv - Genomics 2023Quote: ... directly into ice-cold complete medium (Stemspan serum-free expansion medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies) and 20 ng/ml IL-3 ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
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bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Cell Biology 2023Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Neuroscience 2023Quote: ... NPC monolayers were dissociated to single-cell solutions using accutase solution (STEMCELL Technologies) and subsequently replated at a density of 2x105 cells/cm2 onto Geltrex-coated 6-well plates ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng/μl of BMP4 (STEMCELL Technologies) was added daily for 48 hours ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... myeloid cells were gently thawed and washed in warm myeloid cell media (ImmunoCult™-SF Macrophage Differentiation Medium - using base media with only, M-CSF 50 ng/mL | STEMCELL Technologies, Cat. 10961), and plated in a 24-well low-attachment plate (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Cell Biology 2019Quote: ... FGF (10 ng/ul) (BioLegend or StemCell Technologies) for at least 2 hours ...