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Citations for Stemcell Technologies :
201 - 250 of 252 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... the beads were removed carefully and the agarose wells were coated with a non-adhesive coating solution (07010, Stemcell Technologies) for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
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bioRxiv - Bioengineering 2024Quote: ... each well of the 96 well plate was first coated with 45 μL 2.25% Type I Bovine Collagen Solution (StemCell Technologies) for 1 hour at 37 C ...
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bioRxiv - Bioengineering 2023Quote: ... and broken down to small fragments by a 200uL pipet tip rinsed with Anti-Adherence Rinsing Solution (07010, STEMCELL Technologies). The tissue fragment suspension was transferred into an Anti-Adherence Rinsing Solution coated 15mL conical tube on ice and allowed to sediment by gravity for at least 5 minutes ...
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bioRxiv - Genomics 2022Quote: Removal of red blood cells was done by treating cell pellets with cold ammonium chloride solution (Stem Cell Technologies, 07850) diluted with 1 part PBS-HF ...
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Immunology 2024Quote: ... spleens were mechanically dissociated by a syringe plunger between two pieces of mesh to create a single cell suspension followed by red blood cell lysis by incubation in 0.8% ammonium chloride solution (STEMCELL Technologies) and subsequent staining ...
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bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Neuroscience 2024Quote: ... The spheroid-forming wells were prepared prior to cell seeding by adding 0.5 mL of anti-adherence rinsing solution (07010, STEMCELL Technologies) to each well ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The dissociated glands were spun down at 450 x g for 5 min and resuspended in ammonium chloride solution (STEMCELL Technologies) diluted (4:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... Briefly, PB was collected in FACS buffer (PBS, 2 % FBS, 2 mM EDTA) supplemented with 0.0004 % heparin solution (STEMCELL Technologies Inc.). RBCs was removed by incubation at 37 °C for 25 minutes with 1 % Dextran solution in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... A 10 µM 2X working solution of CalbryteTM AM dye was prepared in sterile BrainPhysTM Imaging Optimized Medium (BPI, STEMCELL Technologies) containing 0.08% pluronic F-127 (#20050 ...
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bioRxiv - Bioengineering 2024Quote: ... non-TC treated 96-well U-bottom plates were passivated with 100 μL anti-adherence rinsing solution (AAR, StemCell Technologies 07010) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 cells were plated in 200 μL media in low-adhesion plates coated with Aggrewell Antiadherence solution (Stem Cell Tech #07010) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were passaged with Versene solution (Paneco, Russia) at a ratio of 1:15 – 1:20 every 4–5 days with ROCK-inhibitor (StemCell Technologies).
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Immunology 2022Quote: ... blood samples were diluted with an equal amount of phosphate buffer saline solution (PBS) and slowly layered on top of the same volume of Lymphoprep density gradient medium (Stemcell Technologies Inc.). After that ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were mechanically detached in VP spheroid medium using a serological pipette before transfer to a 6WP pre-coated with anti-adherence solution (STEMCELL Technologies, 07010). Detached cells from five wells of a 6WP were added divided over six wells of a 6WP ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was finely minced and placed in 15 ml conical tubes with a dissociation solution composed of Collagenase/Hyaluronidase (Stem Cell Technologies) enzymes ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Bioengineering 2024Quote: ... Neutrophils were retained in solution through negative immuno-magnetic separation through the EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Neutrophils were incubated in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Developmental Biology 2024Quote: Embryos were isolated and dissociated to a single cell suspension by incubating in 200ul of dissociation solution consisting of Accutase (Stemcell Technologies, 07922) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... treated monolayers were washed in PBS then incubated in 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) at 37°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... Non-treated petri dishes of 100 mm diameter were coated with 5 mL of anti-adherence rinsing solution (STEMCELL Technologies, Vancouver, CA) for 5 minutes at 37°C to prevent cell adhesion ...
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bioRxiv - Cell Biology 2024Quote: ... 500uL of the cell aggregate suspension were transferred to one well of a 6-well plate previously rinsed with Anti- Adherence Rinsing Solution (STEMCELL Technologies, 07010) containing 2.5mL of N2B27 media ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were passaged with Versene solution (Paneco, Russia) at a ratio of 1:15 – 1:20 every 4–5 days with ROCK-inhibitor (Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... and then seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution following instruction (Stem Cell Technologies, #07010). The culture medium was slightly changed without disturbing aggregates in the V-shape bottom every day and aggregates were collected on day 6 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in serum-free conditions in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753) supplemented with 10 ng/ml basic fibroblast growth factor and 20 ng/ml human epidermal growth factor.
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bioRxiv - Cell Biology 2023Quote: ... buffy coat-PBS-citrate solution was added in portions of 25 ml to tubes filled with 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany). Tubes were centrifuged (20 min at RT ...
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bioRxiv - Genetics 2023Quote: ... we added the following procedures throughout this protocol: (1) we pretreated all plasticware that came into contact with cell aggregates with an anti-adhesion solution (Stem Cell Technology #07010) per manufacturer instructions and (2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 7.5-9.0×104 cells were mixed with 0.5 mL culture medium and seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... the spheroids were harvested into Eppendorf tubes and then transferred to a black 96-well plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies 07010) (∼40 spheroids per well) ...