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Citations for Stemcell Technologies :
2251 - 2300 of 4935 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the cells were spun at 350xg for 10min and the red blood cells were lysed with 5ml Ammonium chloride (StemCell Technologies) per 50ml tube for 5min ...
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bioRxiv - Cell Biology 2021Quote: GMPs were sorted as described earlier from WT (C57Bl6/J) and 50K cells were cultured in 0.5mls of StemSpanTMSFEMII (Stem cell technologies) supplemented with 1% L-Glutamine and Penicillin/Streptomycin in addition to mouse recombinant cytokines ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from HNAE cells apical side (airlift) and provided with Pneumacult ALI basal media from HNAE cells basolateral side (Stemcell technologies). HAE cells were fully differentiated and ready to use after 28 days post-airlift ...
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bioRxiv - Systems Biology 2022Quote: ... and total peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation according to the manufacturers protocol (Lymphoprep, Stem Cell Technologies). Samples were cryopreserved at a concentration of 107 cells per milliliter in a solution of 90% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in IMDM+20%FCS and plated into 1.4 mL of cytokine-rich methylcellulose (Methocult H4435, Stem Cell Technologies). Colonies were scored 14 days later under an inverted microscope ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were dissociated into a single cell suspension with TrypLE and replated on matrigel-coated plates at a density of 40,000 cells/cm2 in mTesR medium (Stem Cell Technologies). Cells were allowed to propagate with a daily change of medium for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2022Quote: NPCs were passaged and plated at 50,000 cells/cm2 in STEMdiff™ Forebrain Neuron Differentiation Media (Stem Cell Tech cat#08600) on Corning® Matrigel® coated tissue culture treated plates and transduced with a NEUROG2 lentivirus (GeneCopoeia cat#LPP-T7381-Lv105-A00-S ...
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bioRxiv - Bioengineering 2019Quote: ... Bulk CD3+ T cells or an isolated CD3+CD8+ T cell subset were extracted from whole blood using negative selection methods (RosetteSep, StemCell Technologies) and cultured in ImmunoCult-XF T cell Expansion Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2019Quote: hESCs were dissociated into single cells using Accutase and 4.5×106 cells were seeded into AggreWell800 plates (STEMCELL Technologies, Vancouver, Canada) to form neural aggregates in STEMdiff Neural Induction Medium (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... To begin the protocol cells were washed twice and incubated in phase I medium comprised of APELII medium (Stem Cell Technologies) supplemented with BMP-4 (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every two days and cells were passaged approximately every three days using ReLeSR (ReLeSR, Stem Cell Technologies #05873), an enzyme free passaging reagent.
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: Tumor tissue excised from the brain was mechanically dissociated using a 70 um cell strainer in phosphate-buffered saline (PBS) with 10% FBS and 0.1 mg/mL DNAse I (Stem Cell Technologies). Cells were then resuspended in 25% isotonic Percoll solution and centrifuged for 20 minutes at 18°C with acceleration and deceleration set to minimum ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Cell Biology 2022Quote: ... DTA or NNMTi iPS cells were resuspended to single cells and seeded into well of Aggrewell 400 plate (Stemcell Technologies #34415) in 1 mL of complete blastoid media ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were removed from single cell suspension using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 0.5-1 x 107 cells/ml in “phase 1 expansion medium” consisting of Stemspan SFEM (Stem Cell Technologies) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were then replated on matrigel-coated plates in mTesR plus media at a density of 40,000 cells/cm2 (Stem Cell Technologies). Cells were allowed to proliferate for two days with a daily media change ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Cancer Biology 2023Quote: ... Bone marrow aspirates were filtered through a cell strainer and mixed with a RosetteSep human MM cell enrichment cocktail (negative selection, STEMCELL Technologies). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... enriched MDA-MB-231 cell line cells or CTCs were stained for EpCAM (1:50, VU1D9, Stemcell Technologies, Vancouver, Canada, AF488) and CD45 (1:25 ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... All co-cultured cells were collected after trypsinization and then plated to duplicate dishes in MethoCult medium (Stem Cell Technologies, H4534) supplemented with 3 units/ml of human EPO (Peprotec ...
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bioRxiv - Neuroscience 2024Quote: ... In vitro differentiation was achieved by sequential culture of the cells in the following media: Step 1 (mTeSR (Stem Cell Tech) + 80 ng/ml BMP4 (PeProtech ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Microbiology 2023Quote: ... Criteria included hESC stemness of at least 80% of double positive cells for both stemness markers SSEA-1 (Stem Cell Technologies) and TRA-1-60 (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Cell Biology 2024Quote: ... The human primed iPSC lines as well as human primed ES cell line H9 (#WA09, obtained from WiCell Research Institute) were maintained without feeder cells either in mTeSR1 (STEMCELL Technologies) on growth factor reduced Matrigel (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... and SK-ES-1 cells were grown and dissociated as above and cells were resuspended in MesenCult MSC basal media (Stemcell Technologies) supplemented with MesenCult MSC Stimulatory Supplement (Human ...
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bioRxiv - Neuroscience 2024Quote: ... or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies) supplemented with 1x Pen/Strep ...
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bioRxiv - Neuroscience 2024Quote: ... cryo-vials of neural progenitor cells were thawed and cells were seeded at a density of 1.25 x 105 cells/cm2 on Matrigel-coated dishes in StemDiff Neural Progenitor medium (Stem Cell Technologies) on day 0 ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Genomics 2024Quote: ... bone marrow cells) fractions using immunomagnetic cells selection targeting CD138 surface expression (automated RoboSep and manual EasySep from StemCell Technologies Inc.). Following magnetic separation ...
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bioRxiv - Cancer Biology 2022Quote: ... isolated from tumours using the EasySep Mouse CD8α Positive Selection Kit (Stemcell Technologies), were harvested from tumour-bearing mice and pulsed with 1 μM peptide corresponding to neoantigens predicted from KPAR1.3 WES (Supplementary Table 1 ...
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bioRxiv - Immunology 2019Quote: ... and then purified with EasySep Human Neutrophil Kit (STEMCELL Technologies Inc. Vancouver, Canada), following manufacturers protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... washed with cold PBS and stained using the ALDEFLUOR Kit (STEMCELL Technologies, Canada) according to a previously described protocol [23] ...
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bioRxiv - Immunology 2021Quote: ... iPSC were differentiated to hematopoietic progenitors using the STEMdiff Hematopoietic Kit (StemCell Technologies) over 12 days ...