Labshake search
Citations for Stemcell Technologies :
2201 - 2250 of 2296 citations for Oxytocin ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
-
bioRxiv - Evolutionary Biology 2021Quote: Diploid and tetraploid iPSC lines were differentiated with the STEMdiff™ Trilineage Differentiation Kit according to the manufacturer’s instructions (STEMCELL Technologies, cat #05230). Differentiation was assessed using reverse transcription quantitative PCR (RT-qPCR ...
-
bioRxiv - Cancer Biology 2021Quote: Spheroid cultures from patient-derived xenograft models were prepared by homogenizing the freshly isolated tumors from mice brains by pipetting in NeuroCult™ NS-A Proliferation Kit (STEMCELL Technologies, 05751). Homogenized tissue is then filtered to get single cell suspension ...
-
bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
-
bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
-
bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Mice were anesthetized with a solution consisting of 200 mg/kg ketamine and 20 mg/kg xylazine and perfused with PBS before neoplastic tissue was processed with the NeuroCult™ enzymatic dissociation Kit (StemCell Technologies, 05715) per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
-
bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
-
bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
-
bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
-
bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
-
bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
-
bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
-
bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
-
bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
-
bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
-
bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
-
bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... After 24 hours cells were washed with D-PBS before media was exchanged for astrocyte differentiation medium (STEMdifftm Astrocyte differentiation kit #100-0013, StemCell Technologies, Cambridge, UK) and a full media exchange was completed every day for 7 days ...
-
bioRxiv - Developmental Biology 2023Quote: PP cells generated using the STEMdiff™ Pancreatic Progenitor Kit and expanded under CINI conditions were dissociated into single cells using AccutaseTM (STEMCELL Technologies, 07920) at 37 °C and then resuspended in PEP medium (Table S4 ...
-
bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
-
bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
-
bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
-
bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... The lungs were collected for single cell suspension and ILC enrichment (by Mouse Pan-ILC Enrichment Kit; STEMCELL Technologies, Vancouver, British Columbia, Canada). The dose of Alt was 30 µg/mouse ...
-
bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
-
bioRxiv - Cell Biology 2022Quote: We isolated CD34+ cells from human cord blood and BM samples using an EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, Canada); sample purity of >95% was confirmed using flow cytometry ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
-
bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...
-
bioRxiv - Immunology 2019Quote: Naïve CD4 T cells were sorted as described above or using EasySep™ Naïve CD4 T cell Isolation Kit (STEMCELL Technologies, Inc., Vancouver, Canada). Equal numbers of cells in serum-free ...
-
bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
-
bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... We confirmed our iPSC cell banks have normal copy number at various mutation hotspots using the hPSC Genetic Analysis Kit (Cat # 07550, STEMCELL Technologies, Vancouver, BC, CAN).