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Citations for Stemcell Technologies :
2101 - 2150 of 2528 citations for Thyroxine T4 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... All tissues were subject to removal of red blood cells (RBCs) using immunomagnetic negative selection with the EasySep RBC Depletion kit (STEMCELL Technologies, 18170). 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Cell Biology 2022Quote: ... iPSC-CM was generated as previously described (ref) using GiWi method (17) or STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (STEMCELL Technologies, 05010), then further purified by lactate selection (18) ...
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bioRxiv - Cancer Biology 2022Quote: ... were harvested from 6-12 weeks old in-house bred BL/6 mice (WT or transgenic CD2 Cre/CD4 Cre mice) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies). HSPCs were transduced with retrovirus for expression of the desired oncogenes ...
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bioRxiv - Developmental Biology 2021Quote: hESCs were single-cell seeded onto 6-well Matrigel-coated plates at 2*106 cells/well for ectoderm and endoderm conditions and 5*105 cells/well for mesoderm condition and treated with the appropriate differentiation medium using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies #05230) according to manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Leukopak-derived peripheral blood mononuclear cells (PBMCs) purchased from LifeSouth Community Blood Centers were debulked with RosetteSep CD8+ enrichment kits (Stem Cell Technologies), and then separated into an enriched fraction of PBMC using a Ficoll gradient ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.
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bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Immunology 2022Quote: Untouched PMNs were isolated from bone marrow in donor mice of the indicated genotype (age 6–8 wk) using EasySep mouse neutrophil enrichment kit (Stemcell Technologies Inc.). In a subset of experiment PMNs from C57/J animals were incubated with 1 mM Dimethyloxalylglycine (DMOG ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Lineage-depleted bone marrow was then stained with an antibody cocktail comprised of a lineage cocktail (BioLegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). 2.5-3.0 × 106 lineage-depleted bone marrow was then washed in PBS and then resuspended in 135 μL Buffer T to which 15 μL of Dre mRNA (at 1 μg/μL ...
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bioRxiv - Cancer Biology 2022Quote: ... single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™, StemCell Technologies, Inc.). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: Neutrophils were isolated from peripheral venous blood using a Percol density gradient as described previously (17) and purified using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies) as per the manufacturer’s instructions (18) ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
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bioRxiv - Immunology 2021Quote: 1 × 105 memory CD4+ T cells freshly purified from PBMC by negative selection and magnetic separetion(EasySep™ Human memory CD4+ T cell enrichment kit, STEMCELL Technologies) were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads ...
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bioRxiv - Immunology 2021Quote: ... Treg were purified from CD4+ memory T cells by magnetic separation (EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, STEMCELL Technologies) and labelled with CellTrace™ Violet ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... from individual mice were pooled and pre-enriched for CD4 T cells using EasySep™ Mouse T Cell Isolation Kit (Stemcell Technologies). Live ...
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bioRxiv - Immunology 2020Quote: ... And pNK cells were isolated from whole blood by negative selection using the EasySep™ Direct Human NK cell Isolation Kit (StemCell Technologies). Following isolation ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then sorted using magnetic beads conjugated with an anti-CD15 antibody (clone MC-480) (Kit easy step, Stem cell Technology).
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bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected after an additional two days and pelleted for dead cell removal based on Annexin V using the Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899).
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bioRxiv - Microbiology 2022Quote: Memory CD4+ T cells were enriched by negative selection using the EasySep Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies #19157) following the recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Primary CD4+ T cells were isolated from spleens for NOD mice using Stemcell murine CD4+ T cell positive selection kit (Stemcell Technologies Inc).
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
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bioRxiv - Neuroscience 2023Quote: Human cerebral organoids were generated from WT and PCDH12-KO RUES1 hESCs and WT GM03651 and GM03652 iPSCs with the STEMDiff Cerebral Organoid Kit (StemCell Technologies, 08570) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...