Labshake search
Citations for Stemcell Technologies :
2101 - 2139 of 2139 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 gp41 1911 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were then resuspended at 1×106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Vancouver, BC, Canada) for 24 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... Spheroid formation media was made in a 3:1 ratio of cell culture media:MethoCult H4100 (StemCell Technologies, Cat#04100, Cambridge, MA, USA), and the total volume was calculated 100µL per well ...
-
bioRxiv - Cancer Biology 2024Quote: ... To obtain single cells the tissue was centrifuged at 450 x g for 3 min and the cell pellet was resuspended in 1 mL Dispase 5 U (Stemcell Technologies, 07913) with 100 μL of DNase I 1 mg/mL (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mouse bone marrow c-Kit+ cells from Rosa26-LSL-Cas9 knockin mice were transduced with the sgRNAs using the lentivirus and were selected for stable expression of the sgRNAs using puromycin (1 μg/ml) in MethoCult™ M3234 (STEMCELL Technologies) supplemented with 10 ng/mL mouse SCF ...
-
bioRxiv - Cell Biology 2024Quote: ... HSPCs were thawed on day 0 into CD34+ expansion media consisting of Stem Span SFEMII supplemented with 1x StemSpan™ CD34+ expansion supplement and 1 μM of U729 (StemCell Technologies). On day 7 ...
-
bioRxiv - Immunology 2024Quote: ... the cells were pelleted at 200 rcf for 5 min and resuspended in 1 ml of Neurosphere Proliferation Medium consisting of NeuroCult Basal Medium (StemCell Tech 05700) containing 5 mL NeuroCult Proliferation Supplement (StemCell Tech 05701) ...
-
Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... organoids were removed from Matrigel and passaged with a split ratio of 1:4 using the protocol published by Stem Cell Technologies for use with their mouse IntestiCult system ...
-
bioRxiv - Immunology 2019Quote: Isolated HSPCs from Apoe−/−Il27ra+/− and Apoe−/−Il27ra−/− mice infused with Ang II were sorted and plated at 1×103 cells in 1ml of MethoCult® GF M3434 (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... and Mononuclear cells were incubated with 1 ml of erythrocytes and the RossetteSep™ CD8+T cell enrichment cocktail to obtain Total CD8+T cells (15063; StemCell Technologies, Canada). The memory cells were eliminated using magnetic beads (8803 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: Testicular organoids were made by mixing germ and somatic cells at a 1:4 ratio and placing them into AggreWell800™ microwells (STEMCELL Technologies, 34811) overnight ...
-
bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
-
bioRxiv - Bioengineering 2021Quote: ... Matrigel was used coat culture dishes for feeder-free expansion of induced pluripotent stem cells and were routinely cultured and maintained in mTeSR™1 (STEMCELL Technologies, 85850) in a humidified incubator at 37 °C with 5% CO2 ...
-
bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared by mechanically disaggregating the domes and digesting the Matrigel for 1 hour using dispase at 37°C (Stem Cell Technologies, 7913). Cells were washed in PBS and resuspended in lysis buffer as previously described (Santiago-Gomez et al ...
-
bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
-
bioRxiv - Cancer Biology 2022Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
-
bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
-
bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
-
bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
-
bioRxiv - Bioengineering 2024Quote: ... The solution was diluted 1:1 with dilution medium and 30 mL was carefully layered on top of 15 mL of Lymphoprep (Cat # 07811, STEMCELL Technologies, Vancouver, Canada) solution ...
-
bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1.25 million SOX2-mTurquoise2 / NANOG-mVenus H9 cells were resuspended in 1 ml of STEMdiff™ Neural Induction Medium (NIM, Stem Cell Technologies) with STEMdiff™ SMADi Neural Induction Supplement (SMADi ...
-
bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
-
bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
-
bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
-
bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
-
bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
-
bioRxiv - Bioengineering 2024Quote: ... Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc, cat#10981) supplemented with 10 ng/mL recombinant IL-2 (StemCell Technologies Inc ...
-
bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)
-
bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
-
bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
-
bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).