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Citations for Stemcell Technologies :
2051 - 2100 of 4374 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Molecular Biology 2023Quote: ... hPSC-derived NPCs were seeded at an initial concentration of 5×105 cells/mL in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and allowed to sit at 37 °C for 48 hrs ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Neuroscience 2023Quote: ... with a cell density of ∼12,000 cells per microwell in an EB formation medium containing neural induction medium (StemCell Technologies, Catalog No. 05835) supplemented with 100 nM of LDN-193189 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Cell were dissociated from large airways or distal lung lobe regions and expanded in basal cell media containing PneumaCult™-Ex Plus medium (Stem Cell Tech) containing 0.5 μM DMH-1 (Tocris #4126) ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were subcultured onto Matrigel at the first passage to avoid loss of the differentiated cells before tolerating the switch to MesenCult-ACF attachment substrate (Stem Cell Technologies, 05448). For passage two or above ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at a density of 1.1×105 cells/well onto a 12 mm Transwell (ref 38023, STEMCELL TECHNOLOGIES, Saint Égrève, France) and grown in KSFM until cell confluency ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
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bioRxiv - Cell Biology 2020Quote: ... CD34+ cells were enriched by EasySep™ from the light-density fraction of Lymphoprep™ or RosetteSep™-depleted CD11b+;CD3+;CD19+ cells (STEMCELL Technologies) and then used after cryopreservation in DMSO and fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2022Quote: Primary CD4+ and CD8+ T cells were isolated from blood of anonymous donors by negative selection using the Human CD4+ T cell isolation kit and Human CD8+ T cell isolation kit (STEMCELL Technologies #17952 and #17953). T cells were cryopreserved in RPMI1640 (UCSF cell culture core ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at 3 × 106 cells/well to allow the embryoid body (EB) formation in Neural Induction Media (NIM) + 10 μM Y-27632 (Stem Cell Technologies, Cat.No. 72308). They were allowed to attach for at least overnight ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...
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bioRxiv - Neuroscience 2022Quote: A single cell suspension of hiNCs and hiACs was prepared by AccutaseTM dissociation and washed once with DMEM/F12 (Stem Cell Tech cat#36254) to remove debris ...
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bioRxiv - Neuroscience 2022Quote: iPSC derived astrocytic cells (hiAC) were differentiated from NPCs by small molecule differentiation in STEMdiff™ Astrocyte Differentiation Media (Stem Cell Tech #cat100-0013) on Corning® Matrigel® coated tissue culture treated plates ...
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bioRxiv - Immunology 2019Quote: Naïve CD4 T cells were sorted as described above or using EasySep™ Naïve CD4 T cell Isolation Kit (STEMCELL Technologies, Inc., Vancouver, Canada). Equal numbers of cells in serum-free ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation PBS was aspirated and the cell pellet was tapped loose and resuspended in mTeSR™-1 spin-EB medium consisting of mTeSR™-1 (Stem Cell Technologies), supplemented with 1 mM Rock-inhibitor (Y27632 ...
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bioRxiv - Microbiology 2021Quote: ... the isolated intestinal tissue was cut into ∼3-to 5-mm pieces and incubated in Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada). After removal of the Gentle Cell Dissociation Reagent ...
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bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Naive and memory CD4+ T cells were isolated from PBMCs using EasySep® naive CD4+ T cell isolation kit and memory CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
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bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
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bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
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bioRxiv - Cancer Biology 2023Quote: HSCs from the BM of CD99 Gt/Gt and littermate WT mice were double sorted to >95% purity and seeded at a density of 150 cells/replicate into cytokine-supplemented methylcellulose medium (Methocult M3434; Stem Cell Technologies, Vancouver, Canada). Colonies number was scored after 10 days ...
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bioRxiv - Developmental Biology 2022Quote: ... were cultured in in TeSR-E8 on Vitronectin XF and were routinely passaged once a week using Gentle Cell Dissociation Reagent (all from Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2024Quote: ... bone marrow cells were collected from female mice (12-16 weeks old) and treated with red blood cell lysis buffer (Ammonium Chloride Solution; Cat# 07800, Stem Cell Technologies; Vancouver, CA). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were dislodged from the AggreWell™ plate by forcefully dispensing DMEM/F12 and applied to a 37 µm cell strainer (Stem Cell Technologies, 27250) to get rid of dead cells.
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bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The assay measures the number of colonies formed in the myeloid-defined media after two weeks in culture using the stem vision plate analyzer (Stem Cell Vision, Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Bioengineering 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were sourced from OriBiotech (Shanghai, China) and cultured in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada). Mouse PBMCs were purified from mouse whole blood by Mouse PBMC Isolation Kit (Solarbio ...
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bioRxiv - Neuroscience 2024Quote: ... was rapidly dissected in ice cold buffer and neural progenitor cells were harvested by centrifugation (according to (41)) and cultured in NeurocultTM proliferation media (Stem Cell Technologies, Cambridge UK) containing recombinant human EGF according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells are resuspended in N2B27 medium supplemented with ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 µm AggreWell plates (Stem Cell Technology, cat# 34425). Once cell structures appear solid ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...