Labshake search
Citations for Stemcell Technologies :
2001 - 2050 of 2193 citations for Human Rh Blood Group D Antigen RHD CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: H9 human ES cell lines obtained from the HMS Cell Biology Initiative for Genome Editing and Neurodegeneration were cultured using the mTeSR1TM1 kit (STEMCELL technology) and 1% penicillin-streptomycin (Sigma ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleens and lymph nodes using the EasySep mouse CD4+ T cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were negatively selected from single cell suspensions using the EasySep™ CD4+ T cell isolation kit (Stemcell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Spleens were processed into single cell suspensions and CD4+ T cells were isolated using the EasySep CD4+ T cell isolation kit (StemCell Technologies) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... we took advantage of the STEMdiff™ trilineage differentiation Kit and followed the manufacturer’s instructions with minor modifications (Cat Nº 05230, Stemcell technologies). hiPSCs were cultured onto Matrigel-coated glass coverslips and on day 0 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, France) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, Canada). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Neuroscience 2024Quote: ... 70,000-80,000 EPSCs were used for the development of individual organoids using the STEMdiff™ Cerebral Organoid Kit (Stemcell technologies, #08570) and the STEMdiff™ Cerebral Organoid Maturation Kit (Stemcell technologies ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from splenocytes by the EasySep Mouse CD4+ T-cell Isolation Kit (#19852, STEMCELL Technologies, Vancouver, Canada) were used to evaluate the capability to differentiate into different T cell subsets as previously described [18,19,20] ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cell isolation was performed with magnetic separation (EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit, StemCell Technologies).
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bioRxiv - Immunology 2024Quote: ... Spleens were macerated through a 70µm mesh filter and cells were separated using the EasySep Mouse B-cell separation kit (StemCell Technologies 19854A). Cells were then treated in B-cell culture media made using 438 mL RPMI 1640 (Gibco 11835-030) ...
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bioRxiv - Immunology 2024Quote: ... n=1 female) mice were prepared by depleting CD4+ cells from thymocytes using the EasySepTM Mouse CD4 Positive Selection Kit II (STEMCELL Technologies). Cells were then incubated with Fixable Viability Dye eFluor™ 780 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... and subjected to a CD8+ T cell negative selection with the EasySepTM Mouse T cell isolation kit (STEMCELL Technologies cat#: 19853), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
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bioRxiv - Bioengineering 2023Quote: ... splenic T lymphocytes were isolated from Cas9 transgenic mice using negative selection with the EasySep Mouse T Cell Isolation Kit (Stemcell Technologies) and then activated with Dynabeads Mouse T-Activator CD3/CD28 beads (Cat # 11456D ...
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bioRxiv - Biochemistry 2023Quote: CD8+ T lymphocytes were enriched from lung cell suspensions prepared according to the manufacturer’s instructions with the CD8+ T cell isolation kit (Stem Cell Technologies). Purified CD8+ T cells were incubated in ELISpot plates in the presence of medium or a pool of S1 peptides (1 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: Patterned differentiation of iPSCs toward neural progenitor cells (NPCs) was conducted using dual-SMAD inhibition (STEMdiff SMADi Neural Induction Kit, STEMCELL Technologies). To promote complete and uniform generation of NPCs ...
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The ALDH assay was performed in accordance with the manufacturer’s instructions for the ALDEFLUOR TM kit (Stem Cell Technologies, Cat # 01700). Cells were trypsinized ...
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bioRxiv - Neuroscience 2023Quote: ... and neural progenitor cells (NPCs) induction was performed according to Stem cell Technologies protocol with STEMdiffTM SMADi Neural Induction kit (STEMCELL Technologies). On Day0 ...
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bioRxiv - Immunology 2023Quote: ... CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies). Cells were resuspended in RPMI + 1% FBS + L-Glutamine + Pen/Strep at a concentration of 1x106 cells/ml and stimulated with 10 µM of pervanadate ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of naïve (CD44loCD62Lhi) lymph node CD8+ T cells (isolated using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit, Stemcell Technologies) were washed with ice-cold PBS supplemented with 2% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells (HPCs) following the manufacturer’s instructions using the commercially available STEMdiff Hematopoietic Kit (Stemcell Technologies, 05310). HPCs in suspension were transferred to plates containing an adherent layer of confluent primary human astrocytes (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ T cells were isolated from the spleens and lymph nodes of OTI mice using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). For direct co-culture experiments ...
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bioRxiv - Neuroscience 2023Quote: ... For microglial differentiation iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (05310, Stem cell Technologies). HPCs were either frozen using Bambanker HRM freezing media (BBH01 ...
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bioRxiv - Immunology 2023Quote: hESCs were differentiated into hematopoietic progenitors using either an embryoid body culture described previously174 or the STEMdiff Hematopoietic Kit (STEMCELL Technologies) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 70 µl of media were removed from each well and replaced by 100 µl embryoid body (EB) formation medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies). On day 2 ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Genetics 2023Quote: ... Common karyotypic abnormalities were measured using the qPCR-based hPSC Genetic Analysis Kit following manufacturer’s instructions (STEMCELL Technologies; cat. no. 07550). Cell lines with true karyotypic abnormalities were not used in this study.
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bioRxiv - Developmental Biology 2023Quote: ... they were collected and moved to a new 96-well plate containing 150 µl of induction media (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) per well ...
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bioRxiv - Developmental Biology 2023Quote: ... 70 µl of media were removed from each well and replaced with 150 µl expansion medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies). On day 10 ...
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bioRxiv - Genomics 2023Quote: Whole blood samples of 5-10 mL were collected in EDTA Vacutainer test tubes and processed for the isolation of erythroid progenitor cells (EPCs) using the Erythroid Progenitor Kit (StemCell Technologies). EPCs were reprogrammed using Sendai particles (CytoTune-iPS 2.0 Reprogramming kit ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The genetic integrity of the engineered hiPSC clones was evaluated by a qPCR-based hiPSC genetic analysis kit (Stem Cell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... The mixed glial cell cultures were separated into a magnetically bound microglia-enriched fraction and pour-off astrocyte-enriched fractions using the EasySep Mouse CD11b Positive Selection Kit (StemCell technologies) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... we immunomagnetically isolated natural Treg from wild type female mice or Foxp3-RFP male and female mice using a CD4+ and CD25+ Treg isolation kit (STEMCELL Technologies) as described below ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, #TIB-9) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, #03800). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...