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Citations for Stemcell Technologies :
151 - 200 of 642 citations for Recombinant Mouse Cd34 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... human recombinant bFGF (hrbFGF) (Stem Cell Technologies, 78003), and heparin (Stem Cell Technologies ...
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bioRxiv - Genomics 2019Quote: ... CD34+ HSPCs were incubated in StemSpan SFEM II medium with StemSpan Myeloid Expansion Supplement (Stem cell technologies, Vancouver, Canada) for 10 days ...
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bioRxiv - Developmental Biology 2022Quote: ... sorted PV CD34+CD36- cells were first expanded in StemSpan SFEM media with StemSpan CC100 (both from StemCell Technologies) for 3 days ...
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bioRxiv - Synthetic Biology 2019Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc., Vancouver, BC, Canada). The resulting CD34+ HSPCs were cultured in StemSpan SFEM II (STEMCELL Technologies Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... 80 µL of StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog #09720) was added to each well ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 7 days and cultured at a density of 2e5-1e6 cells/ml ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼2,000 CD34+Lin-CD38-CD90loCD45RA-EPCR+ cells were cultured on tissue culture plates containing StemSpan II media (Stem Cell Technologies) supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 10/50/500 non irradiated CD34+ cells or 1,500/7,500/15,000 irradiated (3Gy) CD34+ cells per well were plated in 100 μl MyeloCult H5100 medium (STEM CELL technologies). Once a week half medium replacement was done ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog # 09720). Cells were plated on 24-well non-treated plates at a density of 100,000 cells/mL and incubated at 37°C for 2-3 days.
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bioRxiv - Cancer Biology 2024Quote: ... 500 of sorted lentivirally transduced CD34+ HSPCs were seeded into 1 mL of MethoCultTM H4434 Classic media (STEMCELL Technologies #04434) supplemented with StemSpan™ CC100 with antibiotics ...
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bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
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bioRxiv - Cell Biology 2023Quote: ... and 1000 U recombinant human LIF (StemCell Technologies #78055). Medium was replaced daily ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (2 ng/mL, STEMCELL Technologies, #78006.2), antibiotic-antimycotic (1X ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Patient sample mononuclear cells were cultured in RPMI with 10% FBS and 50% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 uM UM729 (StemCell Technologies).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies). All investigations were performed in accordance to the Declaration of Helsinki and informed consent was obtained according to local laws and regulations ...
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were obtained at >95% purity from pooled collections using a two-step Rosette-Sep/EasySep human CD34-positive selection kit (STEMCELL Technologies) according to the manufacturer’s protocols and/or FACS sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Cell Biology 2023Quote: ... For time first division Single cell LIN-CD34+ CD38- CD9+ or CD9- cells sorted in to the round bottomed 96 well plates and culture in SFEM (StemCell Technologies), supplemented with SCF (10ng/ml) ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant epidermal growth factor (StemCell Technologies), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human EGF (10 ug/mL; Stem Cell Technologies) in 10% DMSO before long-term cryopreservation (1X107 cells/mL ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/mL recombinant murine SCF (StemCell Technologies cat. # 78064), and 1 mg/mL polyvinyl alcohol (Sigma cat ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/ml Human Recombinant GDNF (#78058; Stem Cell Technology), 1 mM Dibutyryl-cAMP (#73882 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/ml Human Recombinant BDNF (#78005; Stem Cell Technology), 20 ng/ml Human Recombinant GDNF (#78058 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: Cord blood CD34+ hematopoietic stem cells were purchased and cultured in StemSpan II media with the MK supplemental cytokines (StemSpan II, Stemcell Technologies, Inc.), according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc ...
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bioRxiv - Cancer Biology 2019Quote: ... Low density CD3-CD19-CD11b-cells simultaneously depleted of neutrophils and red cells were isolated on Lymphoprep using RosetteSep™ and the >90% pure CD34+ cells isolated using EasySep (both STEMCELL Technologies). Cells were stored frozen at −176 °C in DMSO with 90% fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2019Quote: Umbilical cord blood Lineage negative (Lin-) and CD34+ cells were obtained using StemSep system according to the manufacturer’s protocol (Stem Cell Technologies, Canada). Lin-CD34+ cells were sorted to obtain HSC (CD34+38-/lowCD45RA-CD90+) ...
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bioRxiv - Cancer Biology 2020Quote: Between 250-500 CD34+ cells were directly sorted into 250 µL of enriched methylcellulose (Methocult H4435 Medium, Stem Cell Technologies, Vancouver, Canada), and then plated into p35 culture dishes containing a final volume of 1.1 mL of enriched methylcellulose ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Immunology 2022Quote: ... Conditionned NSG mice were humanized with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ progenitor cells were enriched using magnetic CD34 microbead kit (Miltenyl Biotec, San Jose, California) and expanded overnight in SFEMII (Stem Cell Technologies) supplemented with SCF (100 ng/mL) ...
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bioRxiv - Immunology 2022Quote: ... 1×103 Lin-CD34+ CD45int CD45RA-CD123-CD90+ cells were sorted based on zmOX40L(±) reporter expression and plated in MethoCult (H4435, Stem Cell Technologies) enriched with rhSCF ...
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bioRxiv - Developmental Biology 2023Quote: CD34+ cells were enriched from de novo progenitor cells collected from each respective 3D hydrogel tissue explant culture using the EasySep Human CD34 Selection (StemCell Technologies, #17856). HUCB CD34+ cells were isolated from whole blood using Lymphoprep (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...