Labshake search
Citations for Stemcell Technologies :
151 - 200 of 1496 citations for QuantiFluo Caspase 3 Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Colony forming assay was performed with growth factor-supplemented methylcellulose (Metho Cult GF 3434, STEMCELL Technologies). 2.5×104 total BM cells ...
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bioRxiv - Cell Biology 2019Quote: ... Clonogenic myeloid progenitors were assessed by standard methylcellulose CFC assays (MethoCult GF M3434; Stem Cell Technologies) using 1.5 × 104 BM mononuclear cells (BMMCs ...
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bioRxiv - Cancer Biology 2023Quote: CFU assays were performed in semi-solid culture using Methocult M3434 (StemCell Technologies, Vancouver, BC, Canada), containing IL-3 ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 3 × 106 single cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8 flex medium supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred at 3 x 106 cells to a well of an AgrreWell800 plate (Stem Cell Technologies) in STEMdiff Neural Induction Medium + SMADi (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... For the first 2 days cells were also exposed to 3 μM CHIR-99021 (Stem Cell Technologies). From day 4 for 5 days ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: ... approximately 3×104 total Ai14 BM cells were plated per well in MethoCult (M3434, Stem Cell Technologies) enriched with rmSCF ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and 100,000 cells were tested in the ALDefluor assay as per the manufacturer’s protocol (Stem Cell Technologies). Cells were incubated with ALDH reagent for 45 min and were analyzed using a BD Accuri ...
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bioRxiv - Developmental Biology 2023Quote: ... and density were analyzed based on representative pictures shown in the CFU assay protocol by STEMCELL Technologies. Subsequently ...
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bioRxiv - Immunology 2023Quote: MethoCult colony-forming unit (CFU) assay was performed with MethoCult H4435-enriched medium (STEMCELL Technologies, Vancouver, Canada) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... with a full medium change at day 2 and passage at day 3 using Accutase (STEMCELL Technologies, 07920).
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bioRxiv - Genomics 2021Quote: ... 25 000 cells were sorted into IntestiCult Organoid Growth Medium (0600-0, −2 and −3 combined, STEMCELL Technologies), centrifuged and directly processed for library generation with the 10x Genomics technology according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ALDH activity was determined for MDA-MB-231 and MCF10A cells using the Aldefluor™ Assay (STEMCELL Technologies) according to manufacturer instructions with incubation conducted for 30min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Colony assays were performed according to manufacturer’s instructions with MegaCultTM-C or MethocultTM H4435 Enriched media (StemCell Technologies) using fresh or cryopreserved HPCs ...
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bioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (Stemcell Technologies) was used to quantify the ALDH enzymatic activity ...
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bioRxiv - Neuroscience 2023Quote: ... StemDiff Hematopoietic Kit (Stemcell Technologies) was used to generated hematopoietic precursor cells (HPCs) ...
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bioRxiv - Immunology 2023Quote: ... Aldefluor kit (01700, Stemcell Technologies) was used for assessing RALDH activity ...
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bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
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bioRxiv - Immunology 2020Quote: ... FcγRII or FcγRIII block was performed by addition of receptor specific mAbs (Stem cell technologies, IV.3 and 3G8) at a 1:100 dilution at the beginning of the incubation period ...
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bioRxiv - Developmental Biology 2023Quote: ... For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir) (Catalog no. 72054, Stem Cell Technologies), 1 μM PD0305901 (Catalog no ...
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bioRxiv - Genetics 2024Quote: 2-3 days post-electroporation HSPCs were plated in SmartDish 6-well plates (cat.: 27370; STEMCELL Technologies, Vancouver, Canada) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat. ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Organoids and xenografts were processed to single cells and labelled using the Aldefluor Assay according to manufacturer’s (Stemcell Technologies) instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Colony forming unit assays were performed using methylcellulose containing media (MethoCult H4434 Enriched and MethoCult SF H4636, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... We prepared triplicate CFU assays by seeding 50,000 cells/1.5 mL of semi-solid Methocult H4435 Enriched media (StemCell Technologies) in 35 mm culture dishes (Greiner ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of ±8.75 × 104 cells/cm2 or 3 × 105 cells/ml in mTeSR medium (Stem Cell Technologies, 85850) supplemented with 1:100 RevitaCell Supplement (Gibco ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Genomics 2023Quote: ... CD14+ monocytes were purified using EasySep Human Monocytes Isolation Kit (negative selection kit; Stem cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ALDEFLUOR kit (StemCell technologies Cat #01700) was used for identification of cancer stem cells that express high levels of ALDH ...
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bioRxiv - Cancer Biology 2020Quote: The Aldefluor kit (Stem Cell Technologies) was used to characterize the ALDH activity of 4T1 cells as described in (Pearce et al ...
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bioRxiv - Molecular Biology 2021Quote: ... All kits are from STEMCELL Technologies, and differentiation was induced following the manufacturer’s protocol.