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Citations for Stemcell Technologies :
151 - 200 of 661 citations for Mouse Anti Hepatitis B Virus E Antigen Antibody 1894 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Immunology 2021Quote: ... and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies, 19054) or naïve B cells (17254) ...
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bioRxiv - Immunology 2021Quote: ... The selected cloned cell lines secreting mAb H5.28 or H5.31 were grown initially in hybridoma growth medium (ClonaCell-HY medium E from STEMCELL Technologies). Prior to antibody expression and purification ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from single cell suspensions by using negative selection kit (Stem Cell Technologies) prior to flow cytometry and cell sorting.
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bioRxiv - Bioengineering 2021Quote: ... The B cells were isolated from fresh blood samples using negative selection kits from Stemcell Technologies (EasySep Direct Human B Cell Isolation Kit) ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... B cells were isolated from PBMCs using a negative selection magnetic separation kit (19054, StemCell Technologies) with at least 70% purity.
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bioRxiv - Cancer Biology 2020Quote: ... Immuno-magnetic T and B cell depletion was performed using the EasySep magnetic system (StemCell Technologies), prior to setting up in vitro cultures ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse organoid culture: we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) derived from the C57BL/6 mouse small intestine ...
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bioRxiv - Immunology 2020Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Immunology 2022Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Cell Biology 2024Quote: ... or 1:20 Alexa488-conjugated anti-TRA-1-60 antibody (7.5 μg/mL final concentration; STEMCELL Technologies). The cells were stained in 100 μL of diluted antibody per 106 cells overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... freshly isolated mammary epithelial cells or transduced cells were cultured in complete Epicult-B medium (STEMCELL Technologies) containing 5 % matrigel (Corning) ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CD8+ T cells were isolated from the mouse spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853A) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse T cells were purified from mouse splenocytes using a negative selection EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies). Mouse splenocytes were prepared from spleens collected from 8-18 weeks old male BL6 mice purchased from Orient bio ...
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bioRxiv - Neuroscience 2019Quote: ... media was supplemented with Y27632, Laminin (2.5 µg per 1ml of media, Thermo-Fisher) and 0.1µM Compound-E (Stem Cell Technologies, Grenoble, France) during the first 3 days after plating in Poly-L-Lysine (Sigma ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse neural stem cells (NSCs) were expanded in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse glioma cells (CPEvIII) were cultured in NeuroCult proliferation medium (mouse) (StemCell Technologies) supplemented with 20 ng/ml EGF ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Growth media was NeuroCult NSC Basal Medium (Mouse) containing NeuroCult Proliferation Supplement (Mouse) (StemCell Technologies), 0.2% heparin solution (Stem-Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Immunology 2019Quote: ... A total of 649 IgGpos/OVApos hybridoma cells were individually deposited into 96-well plates containing Medium E (StemCell Technologies, Vancouver, BC, Canada). Supernatants were screened by ELISA against OVA seven days after sorting ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Cancer Biology 2022Quote: ... was cultured in standard mouse NSC media containing NeuroCult basal medium (mouse & rat, STEMCELL Technologies, #05700) supplemented with NeuroCult proliferation kit (mouse & rat ...
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bioRxiv - Bioengineering 2023Quote: The Mouse IntestiCult medium (STEMCELL Technologies) was prepared and supplemented with 100 U/mL Pen/Strep (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Bioengineering 2020Quote: ... Fluorescence microscopy (Keyence Epi-Fluorescence microscope BZ-X710, Keyence, Japan) was performed using anti-dextran FITC antibody (Stemcell Technologies, Vancouver, Canada) co-incubated with T cells for 24 h in a chamber slide for live cell imaging ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was discarded and the cell pellet was re-suspended in an equivalent volume of HBSS and mixed with 25μL CTC enrichment antibody cocktail (RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, STEMCELL Technologies) at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Untreated peripheral blood B cells were isolated from the PBMCs of healthy donors by negative selection with the RosetteSep for isolation of B cells (StemCell Technologies). All samples were used when they contained ≥95% CD19+ cells ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Immunology 2022Quote: ... and M2A-GFP KI mice (gift of R. Adelstein, NHLBI/NIH) of either sex using negative selection B cell isolation (StemCell Technologies). Euthanasia was performed in accordance with protocols approved by the National Human Genome Research Institute Animal Use and Care Committee at the National Institutes of Health ...